Antihyperlipidemic and Biochemical Activities of Mcy Protein in Streptozotocin Induced Diabetic Rats

Marella, Saritha; Maddirela, Dilip Rajasekhar; Badri, Kameswara Rao; Kumar, Malaka Venkateshwarulu Jyothi; Chippada, Apparao

doi:10.1159/000373954

Cited by 4,313 publications

(13 citation statements)

References 28 publications

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“…Diabetes mellitus was induced in overnight-fasted rats by a single intraperitoneal injection of freshly prepared STZ (50 mg/kg, dissolved in 0.1 M cold citrate buffer, pH 4.5) as described by our group earlier [12, 13]. Diabetes was identified by polydipsia, polyuria and by measuring fasting blood glucose (FBG) levels.…”

Section: Methodsmentioning

confidence: 99%

“…The oral glucose tolerance test was performed in overnight fasted rats as described earlier [12]. Five groups of diabetic rats (n = 6 in each group) were used for this study.…”

Section: Methodsmentioning

confidence: 99%

“…Estimation of blood glucose was carried out using dextrostix with Accucheck glucometer (Glucose oxidase method) [12, 13]. HbA1c was estimated by the method of Eross et al [14].…”

Section: Methodsmentioning

confidence: 99%

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Anti-Diabetic Potential of the Leaves of Anisomeles malabarica in Streptozotocin Induced Diabetic Rats

Kotha

Badri

Nagalapuram

et al. 2017

Cell Physiol Biochem

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Background/Aims: Diabetes mellitus is a pandemic metabolic disorder that is affecting a majority of populations in recent years. There is a requirement for new drugs that are safer and cheaper due to the side effects associated with the available medications. Methods: We investigated the anti-diabetic activity of leaves of Anisomeles malabarica following bioactivity guided fractionation. The different solvent (hexane, ethyl acetate, methanol and water) extracts of A. malabarica leaves were used in acute treatment studies to evaluate and identify the active fraction. The ethyl acetate extract was subjected to further fractionation using silica gel column chromatography and the compounds were identified by LC-SRM/MS and GC-MS. Additional chronic treatment studies were carried out using this active fraction (AMAF) for 30 days in experimental diabetic rats. Fasting blood glucose (FBG), glycosylated hemoglobin (HbA1c), plasma insulin levels and glucose tolerance were measured along with insulin resistance/sensitivity indicators (HOMA-IR, HOMA-β and QUICKI) to assess the beneficial effects of A. malabarica in the management of diabetes mellitus. Results: Among the different solvent extracts tested, ethyl acetate extract showed maximum (66%) anti-hyperglycemic activity. The hexane and ethyl acetate (1: 1) fraction that has maximum anti-diabetic activity was identified as active fraction of A. malabarica (AMAF). The FBG, HbA1c, plasma insulin levels and insulin sensitivity/resistance indicators such as glucose tolerance, HOMA-IR, HOMA-β and QUICKI were significantly improved to near normal in diabetic rats treated with AMAF. Further, we identified key flavonoids and fatty acids as the anti-diabetic active principles from the AMAF of A. malabarica leaves. Conclusion: The results of our study suggest that Anisomeles malabarica has potential anti-diabetic activity in STZ induced diabetic rats.

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Section: Methodsmentioning

confidence: 99%

“…The oral glucose tolerance test was performed in overnight fasted rats as described earlier [12]. Five groups of diabetic rats (n = 6 in each group) were used for this study.…”

Section: Methodsmentioning

confidence: 99%

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Anti-Diabetic Potential of the Leaves of Anisomeles malabarica in Streptozotocin Induced Diabetic Rats

Kotha

Badri

Nagalapuram

et al. 2017

Cell Physiol Biochem

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“…STZ is a toxin that destroys the structure and function of the liver (Marella, Maddirela, Badri, Jyothi Kumar, & Chippada, ). Liver fat deposition reduces the inhibitory effect of insulin on glycogen production and weakens the ability of the insulin to activate glycogen synthase (Samuel et al, ).…”

Section: Resultsmentioning

confidence: 99%

Hypoglycemic effects and biochemical mechanisms of Pea oligopeptide on high‐fat diet and streptozotocin‐induced diabetic mice

et al. 2019

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The aim of this study was to evaluate the hypoglycemic effects of Pea oligopeptide on the glycemic and lipidemic status of mice with type 2 diabetes (T2D) induced by a high‐fat diet and streptozotocin (STZ). Using HPLC‐MS/MS spectra processing, 70 significant peptide (2–3 amino acids) sequences were identified, noting four peptides from Pea oligopeptide with a proline residue at the C‐terminus, which might have dipeptidase‐IV (DPP‐IV) inhibitory activity for the treatment of T2D. After a 4‐week administration of Pea oligopeptide and metformin, various blood biochemical indexes and organic histopathologies were detected to aid the discussion regarding potential mechanisms. The results showed a significant reduction in the levels of blood glucose, lipid profiles, and liver fat deposition in diabetic mice. Furthermore, Pea oligopeptide and metformin improved glucose tolerance, promoted glycogen synthesis, and protected the liver and kidney structures in diabetic mice. The results indicated that Pea oligopeptide played an essential role in the hypoglycemic effect in the T2D mice model. Practical applicationsThis paper examined the preliminary hypoglycemic activities of Pea oligopeptide in a high‐fat diet and STZ‐induced T2D mice. Furthermore, four kinds of dipeptides and tripeptides that might exhibit antidiabetic functions were detected using HPLC‐MS/MS. The results provided practical knowledge regarding the hypoglycemic effects of Pea oligopeptide and established the foundation of its structure–function relationships.

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“…This principle lowered the fasting plasma glucose level in normoglycemic and hyperglycemic rats [Singh and Chandra, 1977] Fruits, Seeds The protein named Mcy isolated from the fruits and seeds of M. cymbalaria lowered the fasting plasma glucose levels in streptozotocin-induced diabetic rats [Rajasekhar et al, 2010]. It also exhibited good antihyperlipidemic action [Marella et al, 2015] and inhibitory actions on aldose reductase and α-glucosidase activities [Marella et al, 2016]…”

Section: Seedsmentioning

confidence: 99%

Antidiabetic plant proteins/peptides as complementary and alternative medicine – analytical perspectives

Marella

2018

Reviews in Analytical Chemistry

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Many of the plant proteins are used as medicinal agents in the treatment of various diseases/disorders as they are produced by using molecular tools of biotechnology. Each protein is unique in its amino acid composition, sequence, subunit structures, size, shape, net charge, iso electric point, solubility, heat stability and hydrophobicity known to play a major role in the isolation and characterization procedures. The study of the protein of interest out of a large number is not possible unless it is obtained in its highly purified and intact form. Extraction, purification and characterization of proteins for different sample types are useful in determining structural, functional and other biological information in the field of pharmacy. Hence, the present review focuses on the sources, isolation, purification and characterization of natural proteins which are proven to be antidiabetic so as to commercialize these drugs (neutraceuticals) to compete with insulin, an ultimate in the treatment of diabetes mellitus.

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Antihyperlipidemic and Biochemical Activities of Mcy Protein in Streptozotocin Induced Diabetic Rats

Cited by 4,313 publications

References 28 publications

Anti-Diabetic Potential of the Leaves of Anisomeles malabarica in Streptozotocin Induced Diabetic Rats

Anti-Diabetic Potential of the Leaves of Anisomeles malabarica in Streptozotocin Induced Diabetic Rats

Hypoglycemic effects and biochemical mechanisms of Pea oligopeptide on high‐fat diet and streptozotocin‐induced diabetic mice

Antidiabetic plant proteins/peptides as complementary and alternative medicine – analytical perspectives

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