Antihyperlipidemic Effect of Solanum incanum on Alloxan Induced Diabetic Wistar Albino Rats

Abstract

The effect of orally administered aqueous fruit extract of Solanum incanum on serum lipid profile of Wistar Albino rats were determined. Twelve male and female Wistar Albino rats were randomly assigned into four groups of three rats each, following acclimatization to laboratory and handling conditions. Diabetes was induced with a single dose of alloxan (120 mg/kg) body weight and plasma glucose was taken 72 h after induction to confirm diabetes. The normal control was not induced. Animals in group a (normal control) and B (diabetic) were administered 0.5 ml of normal saline respectively. Group C was administered with 10 mg/kg weight of glibenclamide and group D was administered 500 mg/kg body weight of aqueous Solanum incanum extract. Extract administration lasted for fourteen days. Water and feeds were allowed ad libitum. After the two weeks treatment with the plant extract, blood samples were collected by cardiac puncture for lipid profile analysis by standard methods and enzyme kits. At the end of week two, the lipid profile of all groups were significantly different. The result on lipid profile showed that the extract treated group was significantly lower (P>0.05) in TC, TAG and VLDL as compared to diabetic control but significantly higher (P<0.05) in HDL and LDL as compared to diabetic control. The glibenclamide treated group was significantly lower (P>0.05) in TAG, I-ID L, and VLDL as compared to the diabetic control but significantly higher (P<0.05) in TC and LDL as compared to diabetic control. However, the extract was observed to have a high significant level of TC, TG, HDL, LDL and VLDL compared to the Normal control. Result on weight showed a significant difference at week and week 2. Antihypercholesterolemic and antihypertigliceridemic effect were observed in this result. Results suggest that Solanum incanum has hypolipidemic effect and therefore makes it beneficial in the dietary management of cardiovascular complications associated with diabetes and that its consumptions are safe for humans.

Reducing Sugar, Alkaloid and Tannin from Dryopteris dilatata Fractions Modulates Diabetogenic and Oxidative Stress Activity on Alloxan Induced Diabetic Rats

“…Equal amounts of the reagents (500 μl) and samples (5 μl) was pipetted at room temperature into labeled tubes (Blank, Standard and Sample), while distilled water was added to the blank in place of the sample. The reagents were mixed and the tubes were allowed to stand for five (5) minutes at 37 0 C. Thereafter, the absorbance of the samples and the standard were read using spectrophotometer at 500 nm against the blank reagent [13,14].…”

“…Missed and allowed to stand for ten (10) minutes at room temperature, after which they were centrifuged for ten (10) minutes at 4000 rpm, and the clear supernatant was separated within two (2) hours. Then, the absorbance of the samples and the standard were read using spectrophotometer at 500 nm [15,14].…”

“…They were mixed and the tubes were incubated for 5 min. at 37 0 C. The absorbance of the samples and standard were read at 500 nm using a spectrophotometer [15,14].…”