Antihypertensive effects of lactoferrin hydrolyzates: Inhibition of angiotensin- and endothelin-converting enzymes

Fernández-Musoles, Ricardo; Salom, Juan B.; Martínez-Maqueda, Daniel; López-Díez, José Javier; Recio, Isidra; Manzanares, Paloma

doi:10.1016/j.foodchem.2012.12.049

Cited by 51 publications

(48 citation statements)

References 31 publications

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“…117 Despite ECE has been shown as a molecular target for pepsin LFH, 92 we did not find significant changes in ECE relative mRNA level or in expression of other genes associated with the ET system. 119 Time-course effect of pepsin LFH in HUVEC on mRNA levels of genes associated with RAS and ET system are necessary to discard any regulation at the mRNA level at shorter exposure times.…”

Section: Effects On the Endothelin Systemcontrasting

confidence: 63%

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Unraveling the mechanisms of action of lactoferrin-derived antihypertensive peptides: ACE inhibition and beyond

et al. 2015

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SummaryHypertension is one of the most important causes of cardiovascular and renal morbidity and mortality, and it represents a serious health problem in Western countries. Over the last few decades scientific interest on food-derived antihypertensive peptides has grown as an alternative to drugs in the control of systemic blood pressure. Most of these peptides target the angiotensin Iconverting enzyme (ACE) but emerging evidence points to other antihypertensive mechanisms beyond ACE inhibition. The milk protein lactoferrin (LF) is a good source of orally active antihypertensive peptides the characterization of which, including ex vivo functional assays and in vivo approaches, shows that they might act on several molecular targets. This review summarizes the mechanisms of action underlying the blood pressure-lowering effects of LF-derived peptides, focusing on their interaction with different components of the renin-angiotensin (RAS) and endothelin (ET) systems. The ability of LF-derived peptides to modify the expression of genes encoding proteins involved in the nitric oxide (NO) pathway and prostaglandin synthesis is also described.3

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Section: Effects On the Endothelin Systemcontrasting

confidence: 63%

“…However, in contrast to in vitro assays of ECE inhibitory effect, pepsin LFH induced higher inhibition of vasoconstriction than that produced by the proteinase K LFH. 92 Table 3 summarizes the inhibitory effects of LF-derived peptides and hydrolysates on ECE activity and ECE-dependent vasoconstriction.…”

Section: Effects On the Endothelin Systemmentioning

confidence: 99%

Unraveling the mechanisms of action of lactoferrin-derived antihypertensive peptides: ACE inhibition and beyond

et al. 2015

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“…The vital system that controls blood pressure is the renin-angiotensin system (RAS). In this system, the main regulators are renin and an angiotensin-Iconverting enzyme (ACE) (Fernández-Musoles et al 2013;He et al 2013). Many researchers have studied the enzymatic hydrolysates of various marine by-products for their antihypertensive activity, such as Hoki skin (Mendis et al 2005), squid skin (Alemán et al 2011b) and Pacific cod skin (Himaya et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and antihypertensive activity of gelatin hydrolysate from Nile tilapia skin

et al. 2014

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Fish skin, a by-product from fish processing industries, still contains a significant amount of protein-rich material. Gelatin was extracted from Nile tilapia skin with the yield 20.77±0.80 % wet weight. Gelatin was then separately hydrolyzed by proteases, including bromelain, papain, trypsin, flavourzyme, alcalase and neutrase. Low molecular weight gelatin hydrolysate (<10 kDa) has a great potential as an antioxidant agent. Flavourzyme hydrolysate has potent activity on ABTS radical scavenging (1,413.61±88.74 μg trolox/ mg protein) and also inhibits the oxidation of linoleic acid at a high level (59.74±16.57 % inhibition). The greatest reducing power is in alcalase hydrolysate (4.951±1.577 mM trolox/mg protein). While, bromelain hydrolysate has the highest ferrous ion chelating activity (86.895±0.061 %). Evaluation of the angiotensin-I-converting enzyme's inhibitory activity indicates that all hydrolysates have great potency as an antihypertensive agent. All studied tilapia skin gelatin hydrolysates contain potent antioxidant and anti-hypertensive effects.

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“…15 The latest study on lactoferr in hydrolyzates (LFHs) generated by trypsin and proteinase K yield different antihypertensive peptides which acts on rennin-angiotensin system (RAS) and the endothelin (ET) system. 16 Black-bone silky fowl (Gallus gallus domesticus Brisson) muscle has been treated by multistage separation. The black-bone silky fowl muscle peptides (BSFP) was hydrolyzed with Alcalase and papain has been tested for ACE in hibitiory activities.…”

Section: Production Of Antihypertensive Peptides From Food Sourcesmentioning

confidence: 99%

Antihypertensive Peptides Derived From Food Sources

Balgir¹

2016

MOJFPT

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Abbreviations: ACE, angiotensin-converting enzyme; RAS, renin-angiotensin system; ENOS, endothelial nitric oxide synthase; NO, nitric oxide; LAB, lactic acid bacteria; SHRs, spontaneously hypertensive rats; ECE, endothelin-1 and endothelin converting enzyme; ET, endothelin; MRW, met-Arg-Trp; FPI, flaxseed protein isolate; RPI, rapeseed protein isolate; BSFP, black-bone silky fowl muscle peptides; DBP, diastolic blood pressure; IER, inhibitory efficiency ratio; AHPM, antihypertensive peptide multimer IntroductionHigh blood pressure or hypertension is a serious medical condition, caused due to a high flow of blood through the blood vessels with a force greater than normal. It strains the heart muscles and damages the blood vessels leading to stroke and finally death, if untreated. 1Untreated hypertension is usually associated with a progressive rise in blood pressure.2 It is the most common cause of morbidity and chronic metabolic disorders in the world. It is estimated to affect billions of individuals worldwide affecting 25% of most adult populations. 3,4 Hypertension as been called a silent killer as it has been implicated as the underlying cause for development of haemorrhagic stroke, cognitive decline, myocardial infarction, heart failure, chronic kidney disease and premature death. 5Hypertension may be classified as essential or secondary. Essential hypertension is the term for high blood pressure with no known etiological cause. Essential hypertension is caused by many factors:ii. lifestyle and environmental influences, andiii. disturbances in vascular structure and neuro-humoral control mechanisms. 5It accounts for about 95% of cases. Secondary hypertension is the term for high blood pressure associated with a known aetiology such as kidney disease, tumours or birth control pills. At present, it is widely accepted that approximately 30-50% of cases of hypertension can arise from genetic susceptibility. Monogenic forms of hypertension provide a unique opportunity to study the effects of single gene and identifying pathways and mechanisms leading to blood pressure elevation. 6 A number of pathways have been involved in the causation of hypertension amongst humans. Prominent among them are the pathways for fluid and electrolyte balance; the reninangiotensin system (RAS), the kinin-kallikrein system, the neutral endopeptidase system and the endothelin-converting enzyme system. Thus in clinical practice, vasodilators, diuretics, calcium channel blockers, angiotensin II receptor blockers and ACE inhibitors have been normally used as drugs.7 Most drugs target one or the other pathways to control hypertension. The response to drugs is varied and thus in non-responders may lead to metabolic disorders like diabetes, vascular blockage etc.Due to a high prevalence of hypertension among human population, a multipronged approach including changes in life-style, dietary approaches and pharmacological treatments is the need of the hour. Peptides of food origin have been reported to play an important prophylactic role in t...

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Antihypertensive effects of lactoferrin hydrolyzates: Inhibition of angiotensin- and endothelin-converting enzymes

Cited by 51 publications

References 31 publications

Unraveling the mechanisms of action of lactoferrin-derived antihypertensive peptides: ACE inhibition and beyond

Unraveling the mechanisms of action of lactoferrin-derived antihypertensive peptides: ACE inhibition and beyond

Antioxidant and antihypertensive activity of gelatin hydrolysate from Nile tilapia skin

Antihypertensive Peptides Derived From Food Sources

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