Antihypertensive Medications Use among Chronic Hemodialysis Patients Visiting the Outpatient Department of Nephrology of a Tertiary Care Centre: A Descriptive Cross-sectional Study

Tuladhar, Lujaw Ratna; Manandhar, Diwakar; Ansari, Sana; Khadka, Nabin; Regmi, Deepak

doi:10.31729/jnma.8095

Cited by 2 publications

(1 citation statement)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Increased cytokine expression in the circulation is characteristic of most patients undergoing ECMO treatment, as respiratory failure is often accompanied by inflammatory conditions, such as pneumonia or sepsis during acute respiratory distress syndrome (ARDS) [13, 14]. ECMO is often used as a treatment for chronic obstructive pulmonary disease (COPD), which is characterized by inflammation [15, 16]. Additionally, it has been reported that ECMO treatment can induce systemic inflammatory response syndrome (SIRS), which leads to increased mortality [17, 18].…”

Section: Introductionmentioning

confidence: 99%

Towards a Biohybrid Lung? Impacted Endothelial Layer Integrity in Inflammatory Conditions

Cheremkhina,

Babendreyer,

Neullens

et al. 2023

Preprint

View full text Add to dashboard Cite

Next to limited hemocompatibility, inflammation and sepsis are frequent complications during extracorporeal membrane oxygenation. Endothelialization of gas exchange membranes has been proposed to overcome these limitations and its general feasibility was demonstrated. However, these investigations focused on application of biohybrid devices under standard culture conditions neglecting patients’ inflammatory status in future application. In this study, we investigate endothelial layer behavior on gas exchange membranes under inflammatory conditions in a microfluidic model system using immunocytochemistry, scanning electron microscopy, flow cytometry and qPCR. While co-culture with peripheral blood mononuclear cells (PBMCs) does not change endothelial layer integrity, confluence of endothelial cells is substantially reduced during inflammation via LPS-activated PBMCs. Cell adhesion molecules were increasingly expressed under inflammatory conditions, consistent with an increased leukocyte adhesion. An upregulation of several genes linked to inflammation is observed: ICAM-1, VCAM-1, E-Selectin, IL6, IL8, IL10, and MCP-1. Our findings suggest that endothelial cells may struggle to maintain layer integrity within a biohybrid device when exposed to inflammatory conditions. This raises the question of whether endothelialization is an effective advancement of current technologies considering inflammation in patients. Yet the presented setup qualifies as sepsis in vitro model replicating the physiological vascular leak phenomenon to aid future investigations in biohybrid lung research.

show abstract