Antihyperuricemic effect of tuna protein hydrolysate and derived products after in vitro digestion or Maillard reaction on oteracil potassium‐induced hyperuricemia rats

Abstract

To investigate the effect of Maillard reaction (MR) and gastrointestinal digestion (GID) on the antihyperuricemic activity of tuna protein hydrolysate (TPH), hyperuricemia rats were treated with TPH, its MR products (TPH-M) and gastrointestinal digesta (TPH-D) in this work. Although the XO inhibitory activity of TPH-M was higher than TPH, TPH-M exhibited even lower serum-uric-acid-lowering activity than TPH. Additionally, there was no significant (P < 0.05) difference observed in the inhibition of XO activity in vitro and antihyperuricemic activity of TPH and TPH-D, indicated that the antihyperuricemic activity of TPH would not decrease after GID. Additionally, TPH, TPH-M and TPH-D could effectively inhibit XO activity and downregulate XO mRNA expression in vivo, suggesting the mechanisms of antihyperuricemic effect was related to XO inhibitory activity and XO mRNA expression. As a whole, TPH could be a useful functional ingredient against hyperuricemia.Antihyperuricemic effect of tuna hydrolysate and derived products Y. Liu et al. 264

Anti‐Hyperuricemic, Nephroprotective, and Gut Microbiota Regulative Effects of Separated Hydrolysate of α‐Lactalbumin on Potassium Oxonate‐ and Hypoxanthine‐Induced Hyperuricemic Mice

“…The tuna flesh hydrolysate produced using alcalase could significantly lower the liver XO activity in PO‐induced hyperuricemic rats. [ 28 ] Ethanol extract of Mesona procumbens in the concentration of 50% reduced the UA level in hyperuricemic mice through modulating XO activity in vivo. [ 29 ]…”

“…The tuna flesh hydrolysate produced using alcalase could significantly lower the liver XO activity in PO-induced hyperuricemic rats. [28] Ethanol extract of Mesona procumbens in the concentration of 50% reduced the UA level in hyperuricemic mice through modulating XO activity in vivo. [29] Owing to its in vivo XO inhibition, gavage of F5 could obviously decrease the serum UA level of hyperuricemic mice like allopurinol, a drug acting as a competitive inhibitor of XO, and the uric acid-lowering effect of F5 was more significant than that of LH-3k.…”

Protective effects of tuna meat oligopeptides (TMOP) supplementation on hyperuricemia and associated renal inflammation mediated by gut microbiota

“…17,18 On the contrary, manipulations of the gut microbiota using probiotics and prebiotics alleviate hyperuricemia and renal inflammation, while the administration of SCFAs improve renal function. [19][20][21] However, previous research on the antihyperuricemia effects of protein hydrolysate was focus on the regulation of uric acid metabolism, especially the inhibition of xanthine oxidase (XOD) activity and transcription, 22 few studies have clarified the mechanism in sight of gut microbiota modulation and clearly determined whether these beneficial effects are mediated via gut microbiota.…”

Research Progress on New Functions of Animal and Plant Proteins

“…Previous studies have found that dietary peptides mainly play a role in reducing UA by inhibiting XOD activity, regulating the expression level of UA transporters, and regulating intestinal microbiota [33]. Animal and plant protein hydrolysates also play a significant role in reducing UA, such as walnut protein hydrolysate [34], tuna protein hydrolysate [35], and whey protein hydrolysate [36]. In addition, the combination of animal and plant proteins may have a superior UA-lowering effect.…”