Antiinflammatory and Antiallergic Activity of Bidens pilosa L. var. radiata SCHERFF

Abstract

The suspension and the boiling water extract of dried powder from the aerial parts of Bidens pilosa L. var. radiata SCHERFF (Tachiawayukisendangusa: MMBP) on the Japanese island of Miyako have antiinflammatory and antiallergic properties in experimental diseases. Oral administration of MMBP suspension in carboxy-methyl-cellulose sodium solution inhibited the production of IgE 10 days after immunization with DNP-ascaris in mice. The extract inhibited histamine release from rat peritoneal mast cells induced by compound 48/80 or antigen-antibody reaction. Oral administration of the suspension inhibited dye exudation in rat skin induced by passive cutaneous anaphylaxis. Oral administration of the suspension inhibited dye exudation in rat skin induced by chemical mediators (histamine, substance P, and serotonin). These findings suggest that MMBP may be clinically useful in the prevention of type I allergic disease.

Improvement of the Antiinflammatory and Antiallergic Activity of Bidens pilosa L. var. radiata SCHERFF Treated with Enzyme (Cellulosine)

“…eMMBP is soluble in water, but was dissolved in 0.25% carboxy-methyl-cellulose sodium (CMC-Na) solution for reference with the effect of MMBP suspension in 0.25% CMC-Na in the previous report. 6) The supernatant of HWex (500 mg/kg, 100 • C, 24 hr) was freeze-dried and dissolved in phosphate-buffered saline (PBS) for comparison with eMMBP and HWex. The animals were administered pharmacologic doses of eMMBP of 100, 250, and 500 mg/kg.…”

“…To determine the titer of anti-DNP-ascaris-IgE, BALB/c mice were injected with DNP-ascaris (5 µg) in alum according to the method described in our previous report. 6) Ten days after injection, the IgE titer against DNPascaris in the serum was determined using ELISA, again according to our previous report. 6) Mice in the cyclophosphamide group received cyclophosphamide (20 mg/kg) p.o.…”

“…6) Ten days after injection, the IgE titer against DNPascaris in the serum was determined using ELISA, again according to our previous report. 6) Mice in the cyclophosphamide group received cyclophosphamide (20 mg/kg) p.o. for 5 days prior to immunization.…”

“…Rats were passively sensitized on the back skin by an injection of anti-DNP-ascaris antiserum (0.1 ml/site, s.c.) according to the method described in our previous report. 6) After 48 hr, the animals were intravenously injected with a mixture of Evans blue dye (0.5%) and DNP-ascaris antigen (1 mg/ml). At 30 min after the antigen challenge, the leaked dye accumulated in the skin was determined colorimetrically at 620 nm.…”

“…We examined the changes in vascular per-meability induced by the inflammatory mediators histamine and substance P according to the previously described method. 6) In this inflammatory model, vascular permeability was estimated by dye leakage in the dorsal skin of rats. The animals were intravenously injected with 50 mg/kg of pontamine sky blue 5 min after injection (0.1 ml/site, s.c.) of the chemical mediators histamine (10 nmol/site) and substance P (1 nmol/site) and saline which spontaneously leaked dye into the back skin.…”