Antiinflammatory effect of low-level laser therapy on Staphylococcus epidermidis endophthalmitis in rabbits

Ma, Wenjiang; Li, Xiaorong; Li, Yingxin; Xue, Zhi-xiao; Hui-juan, Yin; Ma, Hui

doi:10.1007/s10103-011-0991-1

Cited by 10 publications

(8 citation statements)

References 22 publications

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“…The intravitreal space of the left eye was injected with 100 µl of balanced salt solution (BSS) and served as control. The points where the intravitreal injections were made were approximately 2–3 mm from the corneal limbus as suggested in early publications [36] , [37] . All injections were performed by the same researcher to avoid individual operational variations.…”

Section: Methodsmentioning

confidence: 99%

Intraocular Pressure Changes: An Important Determinant of the Biocompatibility of Intravitreous Implants

et al. 2011

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BackgroundIn recent years, research efforts exploring the possibility of using biomaterial nanoparticles for intravitreous drug delivery has increased significantly. However, little is known about the effect of material properties on intravitreous tissue responses.Principal FindingsTo find the answer, nanoparticles made of hyaluronic acid (HA), poly (l-lactic acid) (PLLA), polystyrene (PS), and Poly N-isopropyl acrylamide (PNIPAM) were tested using intravitreous rabbit implantation model. Shortly after implantation, we found that most of the implants accumulated in the trabecular meshwork area followed by clearance from the vitreous. Interestingly, substantial reduction of intraocular pressure (IOP) was observed in eyes implanted with particles made of PS, PNIPAM and PLLA, but not HA nanoparticles and buffered salt solution control. On the other hand, based on histology, we found that the particle implantation had no influence on cornea, iris and even retina. Surprisingly, substantial CD11b+ inflammatory cells were found to accumulate in the trabecular meshwork area in some animals. In addition, there was a good relationship between recruited CD11b+ cells and IOP reduction.ConclusionsOverall, the results reveal the potential influence of nanoparticle material properties on IOP reduction and inflammatory responses in trabecular meshwork. Such interactions may be critical for the development of future ocular nanodevices with improved safety and perhaps efficacy.

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Section: Methodsmentioning

confidence: 99%

Intraocular Pressure Changes: An Important Determinant of the Biocompatibility of Intravitreous Implants

et al. 2011

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“…Coagulase-negative staphylococci are the most common cause, at a rate of 70%, in the etiology of postoperative bacterial endophthalmitis [2]. In our study, we induced experimental endophthalmitis by injecting 0.1 mL (10 3 CFU) S. epidermidis intravitreally into rabbit eyes [10].…”

Section: Discussionmentioning

confidence: 94%

“…The antibiotic disc sensitivity profile of the isolate showed susceptibility to methicillin and vancomycin. The isolate was incubated in 5% sheep blood agar for 24 h to obtain 2.5 mL×4-10 3 colony-forming units (CFU) and was adjusted according to the McFarland scale to prepare bacterial suspensions for inoculation [10]. The rabbits were anesthetized by an intramuscular injection of 50 mg/kg ketamine hydrochloride.…”

Section: Methodsmentioning

confidence: 99%

Intravitreal Infliximab Injection to Treat Experimental Endophthalmitis

et al. 2017

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Objective: The purpose of this study was to compare the use of an intravitreal injection of infliximab and of dexamethasone combined with vancomycin to treat experimental endophthalmitis induced by Staphylococcus epidermidis. Materials and Methods: The study was conducted between March 25 and April 13, 2012. Twenty-five six-monthold healthy rabbits were used, each weighing 2.5-3 kg. The rabbits were randomized into five groups with five animals per group. Endophthalmitis was induced by 0.1 mL (103 colony-forming units) S. epidermidis in all groups. In group 1, injection was not implemented after the occurrence of endophthalmitis. In groups 2, 3, and 4, the following intravitreal injections were given 24 h after the occurrence of endophthalmitis: group 2, 0.1 mg/0.1 mL vancomycin; group 3, 1 mg/0.1 mL vancomycin and 1 mg/0.1 mL dexamethasone; and group 4, 1 mg/0.1 mL vancomycin and 2 mg/0.1 mL infliximab. Group 5 was the control/uninfected group. The rabbits were clinically assessed each day for seven days. On day 9, a histopathologic evaluation was performed after enucleation. Results: After a clinical evaluation, no statistically significant difference was found between the vancomycin+infliximab and vancomycin+dexamethasone groups (p>0.05). The difference was significant when both groups were compared with the vancomycin group (p<0.001). After the histopathologic evaluation, no statistically significant difference was found among the three groups (p>0.05). Conclusion: An intravitreal injection of infliximab and of dexamethasone combined with vancomycin have similar clinical and histopathologic effects. To supplement the antibiotic treatment of endophthalmitis, infliximab in a safe dose range can be used as an alternative to dexamethasone to suppress inflammation and prevent ocular damage. Keywords: Infliximab, dexamethasone, vancomycin, staphylococcus epidermidis, endophthalmitis ÖZ Amaç: Bu çalışmada tavşanlarda Stafilokok epidermidis ile oluşturulan deneysel endoftalmilerde vankomisin ile kombine; intravitreal infliksimab ile intravitreal deksametazonun etkinliklerinin karşılaştırılması amaçlanmıştır. Gereç ve Yöntem: Çalışma 25 Mart 2012-13 Nisan 2012 tarihleri arasında uygulandı. Çalışmada; 6 aylık, 2,5-3 kg ağırlığında, 25 adet sağlıklı tavşan kullanıldı. Denekler herbiri 5 tavşandan oluşan 5 gruba ayrıldı. Grup 1'e; 0,1mL (103 Colony Forming Units) Stafilokok epidermidis ile endoftalmi oluşturulduktan sonra herhangi bir tedavi uygulanmadı. Grup 2, 3 ve 4'e endoftalmi oluşturulduktan 24 saat sonra sırasıyla; 1mg/0,1mL vankomisin, 1mg/0,1mL vankomisin ve 1mg/0,1mL deksametazon, 1mg/0,1mL vankomisin ve 2mg/0,1mL infliksimab intravitreal olarak enjekte edildi. Grup 5 ise kontrol/nonenfekte grup olarak çalışmaya alındı. Denekler 1, 2, 3, 4, 5, 6 ve 7. günlerde klinik olarak değerlendirildi. 9. gün enükleasyon uygulanarak histopatolojik inceleme yapıldı. Bulgular: Klinik değerlendirmede; vankomisin + infliksimab verilen grup ile vankomisin + deksametazon verilen grup kendi arasında karşılaştırıldığında ...

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“…The mechanism of the analgesic (somatic) effect of the laser can partly be related to the release of endogenous endorphins, which has been confirmed in vitro. (25) This effect of laser depends on its intensity and specific wavelength. On the other hand, laser has an anti-inflammatory effect and not only increases the activity of macrophages and neutrophils but also enhances the secretion of specific inflammatory mediators.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Low-Level Laser Versus Transcutaneous Electrical Nerve Stimulation for Treatment of Myofascial Pain Syndrome: A Clinical Trial

Khalifeh¹,

Rezaee

Azizi

et al. 2018

J Res Dentomaxillofac Sci

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Background and Aim: Myofascial pain syndrome (MPS) is the most common cause of facial muscle pain. This study sought to assess the efficacy of transcutaneous electrical nerve stimulation (TENS) and low-level laser (LLL) for treatment of MPS in comparison with a sham laser control group. Materials and Methods: In this randomized controlled clinical trial, 55 MPS patients between 18-60 years old were randomly assigned to TENS, LLL (810 nm) or sham laser group. At the onset of treatment, 500 mg of methocarbamol and 250 mg of naproxen t.i.d. were prescribed for 10 days as the standard treatment protocol. Tenderness and pain of major muscles of mastication, minor muscles, and temporomandibular joint (TMJ) were evaluated using a visual analog scale (VAS). Maximum mouth opening and deviation and deflection of the jaw were also evaluated before and one month after the intervention. Data were analyzed using Kruskal-Wallis test and non-parametric Dunn's test. Results: Pain severity (VAS score) in the masseter, lateral pterygoid, and trapezius muscles (at the side of greater involvement) significantly decreased in both TENS and LLL groups after the treatment. In the sternocleidomastoid (SCM) and anterior temporalis muscles, significant pain reduction only occurred in LLL group. Mouth opening significantly increased in TENS group and decreased in LLL group. TMJ pain significantly decreased only in TENS group. Conclusion: Both TENS and LLL were efficient for treatment of MPS; however, the efficacy of TENS was found to be slightly higher than that of LLL.

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Antiinflammatory effect of low-level laser therapy on Staphylococcus epidermidis endophthalmitis in rabbits

Cited by 10 publications

References 22 publications

Intraocular Pressure Changes: An Important Determinant of the Biocompatibility of Intravitreous Implants

Intraocular Pressure Changes: An Important Determinant of the Biocompatibility of Intravitreous Implants

Intravitreal Infliximab Injection to Treat Experimental Endophthalmitis

Efficacy of Low-Level Laser Versus Transcutaneous Electrical Nerve Stimulation for Treatment of Myofascial Pain Syndrome: A Clinical Trial

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