Antiinflammatory principles of Aconitum roots.

Hara, Hiroshi; Konno, Chohachi; Takata, Hiroshi; Yamada, Yoko; Yamada, Chizuko; Ohizumi, Yasushi; Sugio, Kazuo; Fujimura, Hajimè

doi:10.1248/bpb1978.3.514

Cited by 75 publications

(46 citation statements)

References 6 publications

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“…The pharmacological effects of Aconitum alkaloids include positive inotropic effects, analgesic, anti-inflammatory, and antirheumatic activity as well as neurotransmission actions [2][3][4]. Aconitine and its structurally related analogs are known to injure both the central nervous system and the heart [5].…”

Section: Introductionmentioning

confidence: 99%

Sarcoplasmic reticulum Ca2+ release channel ryanodine receptor (RyR2) plays a crucial role in aconitine-induced arrhythmias

Fu¹,

Li²,

Fan³

et al. 2008

Biochemical Pharmacology

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The present study established a model of RyR 2 knockdown cardiomyocytes and elucidated the role of RyR 2 in aconitine-induced arrhythmia. Cardiomyocytes were obtained from hearts of neonatal Sprague-Dawlay rats. siRNAs were used to down-regulate RyR 2 expression. Reduction of RyR 2 expression was documented by RT-PCR, western blot, and immunofluorescence. Ca 2+ signals were investigated by measuring the relative intracellular Ca 2+ concentration, spontaneous Ca 2+ oscillations, caffeine-induced Ca 2+ release, and L-type Ca 2+ currents. In normal cardiomyocytes, steady and periodic spontaneous Ca 2+ oscillations were observed, and the baseline [Ca 2+ ] i remained at the low level. Exposure to 3 μM aconitine increased the frequency and decreased the amplitude of Ca 2+ oscillations; the baseline [Ca 2+ ] i and the level of caffeineinduced Ca 2+ release were increased but the L-type Ca 2+ currents were inhibited after application of 3 μM aconitine for 5 min. In RyR 2 knockdown cardiomyocytes, the steady and periodic spontaneous Ca 2+ oscillations almost disappeared, but were re-induced by aconitine without affecting the baseline [Ca 2+ ] i level; the level of caffeine-induced Ca 2+ release was increased but L-type Ca 2+ currents were inhibited. Alterations of RyR 2 are important consequences of aconitinestimulation and activation of RyR 2 appear to have a direct relationship with aconitine-induced arrhythmias. The present study demonstrates a potential method for preventing aconitine-induced arrhythmias by inhibiting Ca 2+ leakage through the sarcoplasmic reticulum RyR 2 channel.

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Section: Introductionmentioning

confidence: 99%

Sarcoplasmic reticulum Ca2+ release channel ryanodine receptor (RyR2) plays a crucial role in aconitine-induced arrhythmias

Fu¹,

Li²,

Fan³

et al. 2008

Biochemical Pharmacology

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“…), a traditional herbal medicine, has long been used to treat headache, paralysis, coldness, rheumatism, arthritis, bruises and fractures (Chang et al, 1993). Pharmacological studies relating to antiinflammation, analgesia and cardiotonic effect have been demonstrated in previous studies (Yakazu, 1958;Hikino et al, 1980;Liu et al, 1983;Chen et al, 1990;Oyama et al, 1994). It was also reported that Aconiti tuber showed a therapeutic effect on endotoxin shock in the cat (Zhou and Liu, 1988).…”

Section: Introductionmentioning

confidence: 98%

Aconiti tuber (Bushi) improves microcirculatory disturbances induced by endotoxin in rats

2000

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“…BMC, BAC and BHC are representatives of MDAs, which have anti-inflammatory, anti-allergic, anti-oxidant and hepatoprotective activities (Di Filippo et al, 2010;Hikino et al, 1980;Tayal et al, 1982). A better understanding of the process in vivo of multiherbal medicines would help clarify the synergy of multiple components, illustrate the compatibility mechanism of multiherbal medicine and link data from pharmacological assays to clinical effects.…”

Section: Introductionmentioning

confidence: 99%

Comparative pharmacokinetics of three monoester‐diterpenoid alkaloids after oral administration of Acontium carmichaeli extract and its compatibility with other herbal medicines in Sini Decoction to rats

Hai

Liu

Zhang

et al. 2014

Biomedical Chromatography

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Sini decoction (SND) is an important traditional Chinese multiherbal formula, which is widely used to treat cardiovascular disease. Acontium carmichaeli (AC) is a leading herb in SND, whose main components are monoester-diterpenoid alkaloids (MDAs). The aim of this study is to compare the pharmacokinetics of three MDAs in rat plasma after oral administration of AC extract and its compatibility with other herbal medicines in SND. A sensitive, accurate and specific LC-MS/MS method was developed to determine the contents of three MDAs in rat plasma. Male Sprague-Dawley rats were randomly assigned to four groups: AC, AC + ZO, AC + GU and SND groups. There were significant differences in the pharmacokinetic parameters (Cmax, Tmax, t1/2, AUC(0-24), MRT and CL). Compared with the AC group, Cmax, AUC(0-24) and CL of three MDAs increased and t1/2 decreased in AC + ZO, AC + GU and SND groups. Little changed in the AC + GU group in comparison with AC + ZO group, which indicated that other ingredients in ZO may promote the absorption rate and accelerate excretion rate of MDAs. The results could be helpful for revealing the compatibility mechanism of Chinese multiherbal medicine and providing clinical medication guidance on AC and its compatibility with other herbal medicines in SND.

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Antiinflammatory principles of Aconitum roots.

Cited by 75 publications

References 6 publications

Sarcoplasmic reticulum Ca2+ release channel ryanodine receptor (RyR2) plays a crucial role in aconitine-induced arrhythmias

Sarcoplasmic reticulum Ca2+ release channel ryanodine receptor (RyR2) plays a crucial role in aconitine-induced arrhythmias

Aconiti tuber (Bushi) improves microcirculatory disturbances induced by endotoxin in rats

Comparative pharmacokinetics of three monoester‐diterpenoid alkaloids after oral administration of Acontium carmichaeli extract and its compatibility with other herbal medicines in Sini Decoction to rats

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