Antileishmanial Drug Miltefosine‐dsDNA Interaction <i>in situ</i> Evaluation with a DNA‐Electrochemical Biosensor

Machini, Wesley B. S.; Oliveira-Brett, Ana Maria

doi:10.1002/elan.201700581

Cited by 13 publications

(7 citation statements)

References 40 publications

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“…Miltefosine belongs to the class of alkylphosphocholine drugs, and presents activity against various parasite species, pathogenic bacteria and fungi, and cancer cells, and is currently used to treat visceral, cutaneous, and mucosal forms of the parasitic disease leishmaniasis. The miltefosine–dsDNA interaction was investigated with a GCE, in incubated solutions and at dsDNA electrochemical biosensors [ 80 ], by following the changes in the G r and A r oxidation peaks, and the appearance of the free G oxidation peak, as shown in Figure 5 . The DPV results showed that the miltefosine–dsDNA interaction occurs either independently of the dsDNA sequence, leading to the condensation/aggregation of DNA strands and producing a rigid miltefosine–dsDNA complex, or preferentially at the G bases, causing the release of free G. However, miltefosine did not cause oxidative DNA damage.…”

Section: Dna Electrochemical Biosensors For the Detection Of Pharmmentioning

confidence: 99%

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DNA Electrochemical Biosensors for In Situ Probing of Pharmaceutical Drug Oxidative DNA Damage

Chiorcea-Paquim

Oliveira-Brett

2021

Sensors

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Deoxyribonucleic acid (DNA) electrochemical biosensors are devices that incorporate immobilized DNA as a molecular recognition element on the electrode surface, and enable probing in situ the oxidative DNA damage. A wide range of DNA electrochemical biosensor analytical and biotechnological applications in pharmacology are foreseen, due to their ability to determine in situ and in real-time the DNA interaction mechanisms with pharmaceutical drugs, as well as with their degradation products, redox reaction products, and metabolites, and due to their capacity to achieve quantitative electroanalytical evaluation of the drugs, with high sensitivity, short time of analysis, and low cost. This review presents the design and applications of label-free DNA electrochemical biosensors that use DNA direct electrochemical oxidation to detect oxidative DNA damage. The DNA electrochemical biosensor development, from the viewpoint of electrochemical and atomic force microscopy (AFM) characterization, and the bottom-up immobilization of DNA nanostructures at the electrode surface, are described. Applications of DNA electrochemical biosensors that enable the label-free detection of DNA interactions with pharmaceutical compounds, such as acridine derivatives, alkaloids, alkylating agents, alkylphosphocholines, antibiotics, antimetabolites, kinase inhibitors, immunomodulatory agents, metal complexes, nucleoside analogs, and phenolic compounds, which can be used in drug analysis and drug discovery, and may lead to future screening systems, are reviewed.

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Section: Dna Electrochemical Biosensors For the Detection Of Pharmmentioning

confidence: 99%

“… Baseline-corrected DPV at pH 4.5, obtained with a dsDNA–electrochemical biosensor, (red line) before and (black line) after incubation with 0.1 μM miltefosine, during different time periods. Adapted from [ 80 ] with permission. …”

Section: Figures and Schemesmentioning

confidence: 99%

DNA Electrochemical Biosensors for In Situ Probing of Pharmaceutical Drug Oxidative DNA Damage

Chiorcea-Paquim

Oliveira-Brett

2021

Sensors

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“…The investigations of the interactions are depending on the differences in the electrochemical behaviour of DNA such as the decreases/increases of the peak currents and the dislocations of the potentials associated with guanine and adenine. DNA modified probes offer great sensitivity for the detection of even small changes in the structure of DNA and have been successfully used to describe the interaction of the anticancer drugs with DNA .…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Determination of the Effect of Caffeic Acid onto the Interaction between Idarubicin and DNA by Single‐use Disposable Electrodes

Öndeş

Muti

2020

Electroanalysis

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Now it is well known, antioxidant may affect the interaction of anticancer drugs and DNA. This study aims to investigate the interaction between Idarubicin and DNA and the effect of caffeic acid on this interaction. Disposable, inexpensive, easy handle electrodes were used in this study to investigate the interaction of idarubicin and DNA electrochemically. Idarubicin (IDR) is an anthracyline antitumor antibiotic, which used against one or more types of leukemia. Electrochemical behaviour of IDR was investigated by using cyclic voltammetry. The interaction between anticancer drug, IDR and calf thymus double‐stranded DNA (ctdsDNA) was investigated by using differential pulse voltammetry (DPV) technique in the absence and presence of caffeic acid.

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“…The METÀ dsDNA interaction mechanisms research is also important due to the possibility to better understand, as well as detecting, perturbations of the DNA doublehelical structure and oxidative DNA damage. The DNAelectrochemical biosensor [29][30][31][32][33][34][35][36], consists of an electrochemical transducer with DNA immobilized on its surface capable of detecting specific drugÀ DNA binding process [37,38], through electrochemical transduction.…”

Section: Introductionmentioning

confidence: 99%

Antidiabetic Drug Metformin Oxidation andin situInteraction with dsDNA Using a dsDNA‐electrochemical Biosensor

2019

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The antidiabetic drug metformin (MET) is one of a group of emerging pharmaceutical drug contaminants in the wastewater treatment plants. The electrochemical behaviour of MET−Cu(II) complex by differential pulse and square wave voltammetry, in a wide pH range, at a glassy carbon electrode modified with a carbon black dihexadecylphosphate film (CB−DHP/GCE), was investigated. The MET−Cu(II) complex oxidation showed one pH‐dependent process, which leads to the formation of an oxidation product, being oxidized at a lower potential. The electroanalytical MET−Cu(II) complex detection limit, LOD=0.63 μM, and quantification limit, LOQ=2.09 μM, were obtained, and the MET−Cu(II) complex determination in wastewater samples collected from a senior residence effluent, using the CB−DHP/GCE, was achieved. Considering MET toxicity, the electrochemical evaluation of MET−dsDNA interaction, in incubated solutions and using dsDNA‐electrochemical biosensors, following the changes in the oxidation peaks of guanosine and adenosine residues electrochemical currents, was also investigated. The MET−dsDNA interaction mechanism, for shorter times, occurs by the binding of MET molecules in the minor grooves of the dsDNA, and for long times, the stabilization of the MET−dsDNA complex, causing a local distortion and/or unwinding of dsDNA morphology, is described. However, MET did not promote DNA oxidative damage.

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Antileishmanial Drug Miltefosine‐dsDNA Interaction in situ Evaluation with a DNA‐Electrochemical Biosensor

Cited by 13 publications

References 40 publications

DNA Electrochemical Biosensors for In Situ Probing of Pharmaceutical Drug Oxidative DNA Damage

DNA Electrochemical Biosensors for In Situ Probing of Pharmaceutical Drug Oxidative DNA Damage

Electrochemical Determination of the Effect of Caffeic Acid onto the Interaction between Idarubicin and DNA by Single‐use Disposable Electrodes

Antidiabetic Drug Metformin Oxidation andin situInteraction with dsDNA Using a dsDNA‐electrochemical Biosensor

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