Antilithiasic and Hypolipidaemic Effects ofRaphanus sativusL.var. nigeron Mice Fed with a Lithogenic Diet

Abstract

In Mexico, Raphanus sativus L. var. niger (black radish) has uses for the treatment of gallstones and for decreasing lipids serum levels. We evaluate the effect of juice squeezed from black radish root in cholesterol gallstones and serum lipids of mice. The toxicity of juice was analyzed according to the OECD guidelines. We used female C57BL/6 mice fed with a lithogenic diet. We performed histopathological studies of gallbladder and liver, and measured concentrations of cholesterol, HDL cholesterol and triglycerides. The juice can be considered bioactive and non-toxic; the lithogenic diet significantly induced cholesterol gallstones; increased cholesterol and triglycerides levels, and decreased HDL levels; gallbladder wall thickness increased markedly, showing epithelial hyperplasia and increased liver weight. After treatment with juice for 6 days, cholesterol gallstones were eradicated significantly in the gallbladder of mice; cholesterol and triglycerides levels decreased too, and there was also an increase in levels of HDL (P < 0.05). Gallbladder tissue continued to show epithelial hyperplasia and granulocyte infiltration; liver tissue showed vacuolar degeneration. The juice of black radish root has properties for treatment of cholesterol gallstones and for decreasing serum lipids levels; therefore, we confirm in a preclinical study the utility that people give it in traditional medicine.

Effects of Glucosinolate-Enriched Red Radish (Raphanus sativus) on In Vitro Models of Intestinal Microbiota and Metabolic Syndrome-Related Functionalities

“…In vitro and in vivo studies carried out with a radish extract alone or mixed with other vegetables have observed an improvement of some obesity-related parameters, such as the accumulation of fat or the modulation of adipokines such as adiponectin. 106 , 110 Thus, the results obtained regarding the decrease in interleukin IL-6 are consistent with the literature.…”

“…The intracellular level of accumulated lipids was not modified with the treatment (Mbasal: 1.00 ± 0.11 vs Mtt: 1.02 ± 0.13); however, ADIPOQ showed a tendency toward reduced levels (Mbasal: 1.00 ± 0.15 vs Mtt: 0.77 ± 0.21), and IL-6 showed a significant reduction (Mbasal: 1.00 ± 0.28 vs Mtt: 0.031 ± 0.004) (Figure ). In vitro and in vivo studies carried out with a radish extract alone or mixed with other vegetables have observed an improvement of some obesity-related parameters, such as the accumulation of fat or the modulation of adipokines such as adiponectin. , Thus, the results obtained regarding the decrease in interleukin IL-6 are consistent with the literature.…”

“…Lipidation of apolipoprotein A-I (apoA-I) by ABCA1 is the rate-limiting step in RTC and generates plasma HDL. 105 This is consistent with in vivo studies in rats, 106 suggesting a lipid-lowering and an antiatherosclerosis effect from radish, as it may help to reduce plasma cholesterol levels while increasing HDL.…”

Progress and prospects in the management of kidney stones and developments in phyto-therapeutic modalities

“…Its pharmacological activities are antiurolithiasic, antimutagenic, antimicrobial, anticancer, antiproliferative hepatoprotective, and antioxidant. Castro-Torres et al 10 reported its efficacy in kidney stones.…”

Beneficial effects of the consumption of sun‐dried radishes ( Raphanus sativus cv. YR‐Hyuga‐Risou) on dyslipidemia in apolipoprotein E‐deficient mice

“…Some people drink radish juice in pursuit of certain health benefits. One study showed that drinking radish juice lowered blood cholesterol and triglyceride levels in mice compared with the control group, which only drank water (Castro-Torres et al, 2012). However, little information is available regarding the effects of radishes themselves.…”