Antilung Antibodies in Asthma

Abstract

Twenty-four asthmatic patients were evaluated for the presence of circulating cytotoxic lung antibodies. These patients were further classified as either extrinsic or intrinsic asthmatics on the basis of skin testing, age of onset, and atopic history. Of the 12 patients considered to have extrinsic asthma, 10 had positive titers, one borderline, and one negative for cytotoxic lung antibodies. In the group of 12 patients classified as intrinsic asthmatics, eight had negative titers, two borderline, and two positive for cytotoxic lung antibodies. Adsorption studies indicated that these antibodies were organ-specific. Twenty normal non-smoking controls were also tested, all of whom were negative. Five patients with allergic rhinitis, positive intradermal skin tests, and no history of asthma were found to be negative for cytotoxic lung antibodies.

Antibodies to synovial‐derived cells in patients undergoing artificial prosthesis implants

“…The technique used for the detection of complement-dependent cytotoxic antibodies, as determined by membrane damage and measured by cellular uptake of trypan blue, has been previously described (5)(6)(7)(8).…”

“…A CO, incubation system not only facilitated manipulation of the plates, but also seemed to enhance the vigor of the target cells. Based on previous experience, we consider any titer above 1:2 as significant (5)(6)(7)(8).…”

“…The presence, however, of an antisynovial antibody has not been described in association with artificial prosthesis. Consequently, we decided to utilize a technique which we had used successfully in detecting putative antibodies to lung and kidney in relation to transplantation and disease states (5)(6)(7)(8). In this study, we modified the technique and employed target cells derived from human synovium to determine if synovial antibodies were associated with artificial prosthesis implantation.…”