Antimalarial activity of Sida acuta Burm. f. (Malvaceae) and Pterocarpus erinaceus Poir. (Fabaceae)

Karou, Damintoti Simplice; Dicko, Mamoudou H.; Sanon, Souleymane; Simporé, Jacques; Traoré, Alfred S.

doi:10.1016/j.jep.2003.09.010

Cited by 122 publications

(84 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The majority of the Togolese people living in the rural areas traditionally use plants for food and medicine. As in the other African countries, in recent years, the plants used traditionally for therapeutic purposes have attracted the attention of researchers (Karou et al, 2003(Karou et al, , 2005(Karou et al, , 2006Koudouvo et al, 2011;Tchacondo et al, 2011). Although ethnobotanical surveys are essential in providing new leads for pharmacological screenings (Natarajan et al, 2000;Polesna, et al, 2011), very few studies have been conducted in Togo.…”

Section: Introductionmentioning

confidence: 99%

Ethnobotanical study of medicinal plants used in the management of diabetes mellitus and hypertension in the Central Region of Togo

Karou¹,

Tchacondo²,

Tchibozo³

et al. 2011

Pharmaceutical Biology

Self Cite

130

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Ethnobotanical study of medicinal plants used in the management of diabetes mellitus and hypertension in the Central Region of Togo

Karou¹,

Tchacondo²,

Tchibozo³

et al. 2011

Pharmaceutical Biology

Self Cite

130

View full text Add to dashboard Cite

“…Classification of the fraction activity or plant extract was classified into 4 groups according their IC 50 for antiplasmodial activity test. [16][17][18] Very active anti plasmodial has IC 50 less than 5 µg/mL; active anti plasmodial has IC 50 less 5-50 µg/mL; less active anti plasmodial has IC 50 less than 51-100 µg/mL ; inactive antiplasmodial has IC 50 more than 100 µg/mL.…”

Section: Discussionmentioning

confidence: 99%

ANTI-PLASMODIUM FALCIPARUM IN VITRO ACTIVITY OF CALOPHYLLUM BICOLOR EXTRACT: Morphology and Ultra Structure

et al. 2015

View full text Add to dashboard Cite

Background: Anti-malarial resistance of Plasmodium, such as chloroquine becoming a health problem worldwide. This research aims to evaluate the in vitro anti-plasmodium activity of hexane fraction extract of Calophyllum bicolor (C. bicolor) against culture of Plasmodium falciparum (P. falciparum) 3D7 and determine its inhibitor concentration of 50% (IC 50 ); to observe the microscopic changes of P. falciparum after exposed to the hexane fraction of C. bicolor extract and to observe the ultra structure changes of P. falciparum 3D7 after being exposed to hexane fraction of C. bicolor extract. Method: This research applied quasi experimental with post-test only control group design. Anti malarial activity test of hexane fraction of C. bicolor extract towards Plasmodium falciparum 3D7 2% was undertaken over 48 hours with in vitro incubation technique. The level of parasitemia was observed using binocular optical microscope with 1000x magnification by counting the infected erythrocytes with Giemsa color technique. Then it was analyzed to determine the level of inhibitor concentration of 50% (IC 50 ) of the C. bicolor extract. The next step is to observe the changes of parasite's morphologic and ultra structure after the 24 and 48 hours incubation of the parasite P. falciparum 3D7 with hexane fraction of C. bicolor, IC 50 . Then, the morphologic change of P. falciparum 3D7 was observed with optical binocular microscopy and the ultra structure changes with Transmission Electron Microscopy (TEM). The changes of morphologic and ultra structure were analyzed qualitatively. Results: The research revealed that hexane fraction of the C. bicolor extract inhibited the growth of the parasite P. falciparum 3D7 with the value of -49.00±2.54, 12.53±3.13, 23.01±1.10, 27.68±4.23, 48.65±18.71, 70.82±4.67, 80.52±6.17% with the hexane extract concentration of 0, 0.001, 0.01, 0.1, 1, 10, 100, and 1000 µg/mL. This research also found that the inhibitor concentration of 50% (IC 50 ) of hexane fraction of C. bicolor extract was 3.94 µg/mL in 10 -7 which could change the morphologic and ultra structure of P. falciparum 3D7. There were several changes in morphology of the parasite over 24 hour incubation compared with the control group, i.e. the nucleus of young tropozoid became thicker, darker, and smaller; the skizon were condensate, thicker, darker, karyorrhexis and demolition of membrane. Hexane fraction of C. bicolor extract also changes the ultra structure over 24 hour incubation, i.e. vacuole membrane boundary and hemozoin were unclear. Over 48 hours of incubation the nucleus and cytoplasm bigger than those in the control group. Conclusion: Hexane fraction of the C. bicolor extract showed a strong anti plasmodium activity towards the in vitro culture of P. falciparum 3D7 at the level of (IC 50 ) 3.94 µg/mL. The Hexane fraction of C. bicolor extract could change the morphology and ultra structure of P. falciparum 3D7 in vitro.

show abstract

“…Indeed, THs have long experience in the management of the disease. Many Togolese medicinal plants have been screened for the in vitro antiplasmodial activity (Karou et al, 2003;Gbeassor et al, 1989). These screenings often show scientific rational behind plant usage in the management of malaria by Togolese TH.…”

Section: Discussionmentioning

confidence: 99%

Herbal Remedies And Their Adverse Effects In Tem Tribe Traditional Medicine In Togo

Tchacondo¹,

Karou²,

Batawila³

et al. 2010

Afr. J. Trad. Compl. Alt. Med.

View full text Add to dashboard Cite

In Africa, up to 80% of the population relies on herbal concoctions for their primarily health care. In Togo, western Africa, Tem tribe is a population with old knowledge of medicinal plants, however, still very little is known about their medical practices. The present study was conducted to access for the apprehension of adverse effects of traditional remedies by Tem traditional healers (TH). Enquiry was performed by interviews with healers from August to October 2007 in Tchaoudjo prefecture (Togo). The study allowed us to interview 54 TH including 41(75.93%) males and 13(24.07%) females, who cited 102 recipes assumed to have adverse effects. The recipes were used alone to cure several diseases including haemorrhoids (22.55%), female sexual disorders and infertility (21.57%), gastrointestinal disorders (18.63%), and malaria (6.86%). A total of 34 plants belonging to 21 families were cited to be components of the recipes. Euphorbiaceae and Mimosaceae families were the most represented, however, Nauclea latifolia, Khaya senegalensis, Pseudocedrela kotschyi and Xeroderris stuhlmannii were the main components of recipes linked to adverse effects. A total of 20 adverse effects were linked to the administration of theses drugs, and among them; diarrhoea, abdominal pains, polyuria, general weakness and vomiting were the most frequently encountered. These findings were in accordance with several reports of the literature concerning medicinal plants, although they were based on empirical observations. Laboratory screenings are needed to access for the effectiveness as well as the possible toxic effects of the recipes.

show abstract

Antimalarial activity of Sida acuta Burm. f. (Malvaceae) and Pterocarpus erinaceus Poir. (Fabaceae)

Cited by 122 publications

References 14 publications

Ethnobotanical study of medicinal plants used in the management of diabetes mellitus and hypertension in the Central Region of Togo

Ethnobotanical study of medicinal plants used in the management of diabetes mellitus and hypertension in the Central Region of Togo

ANTI-PLASMODIUM FALCIPARUM IN VITRO ACTIVITY OF CALOPHYLLUM BICOLOR EXTRACT: Morphology and Ultra Structure

Herbal Remedies And Their Adverse Effects In Tem Tribe Traditional Medicine In Togo

Contact Info

Product

Resources

About