Antimalarial Constituents fromNauclea orientalis (L.) L.

Abstract

Bioassay-guided fractionation of the antimalarial-active CHCl3 extract of the dried stem of Nauclea orientalis (L.) L. (Rubiaceae) has resulted in the isolation of two novel tetrahydro-beta-carboline monoterpene alkaloid glucosides, naucleaorine (= (16alpha,17beta)-3,14:15,20-tetradehydro-16-ethenyl-17-(beta-D-glucopyranosyloxy)-19alpha-methoxyoxayohimban-21-one; 1) and epimethoxynaucleaorine (2), as well as the known compounds, strictosidine lactam (= (15beta,16alpha,17beta)-19,20-didehydro-16-ethenyl-17-(beta-D-glucopyranosyloxy)oxayohimban-21-one; 3), 3,4,5-trimethoxyphenol (4), 3alpha-hydroxyurs-12-en-28-oic acid methyl ester (5), 3alpha,23-dihydroxyurs-12-en-28-oic acid (6), 3alpha,19alpha,23-trihydroxyurs-12-en-28-oic acid methyl ester (7), and oleanolic acid (8). Compounds 1, 2, 6, and 8 showed moderate in vitro activities against Plasmodium falciparum. Their structures and configurations were elucidated by spectroscopic methods including 1D- and 2D-NMR analyses.

Antimalarial activity and toxicity evaluation of a quantified Nauclea pobeguinii extract

“…However, in the present study strictosamide was found to be inactive in vitro (IC 50 > 64 M). Our results are in agreement with those obtained by Camacho et al (2004) and He et al (2005), who reported IC 50 values for strictosamide (or strictosidine lactam) of 547 g/ml (Plasmodium falciparum strain K1) and >20 M (strains D6 and W2). Indeed, it is rather unlikely that a glycosidic compound may enter the parasite and reach an intracellular target.…”

Pemanfaatan Tumbuhan Obat oleh Suku Kanum di Taman Nasional Wasur, Papua

“…Komponen metabolit sekunder utama yang terkandung dalam Caesalpinia bonduc antara lain alkaloid, flavanoid, saponin, tannin dan triterpenoid (Singh & Raghav, 2012) sedangkan senyawa aktifnya yang tergolong diterpen telah diketahui bersifat anti malaria Plasmodium falciparum (Linn et al, 2005). Sementara itu, bagian kulit batang Nauclea orientalis dimanfaatkan pula sebagai obat malaria oleh masyarakat Togo Afrika (Bahekar & Kale, 2013) dan secara farmakologi telah diketahui bersifat anti malaria dengan kandungan senyawa bioaktif yang bersifat anti parasit malaria Plasmodium falciforum antara lain naucleaorine dan ephymetoxynaucleaoine (He et al, 2005;Deharo & Ginsburg, 2011;Soejarto et al, 2012).…”

Revisiting the linkage between ethnomedical use and development of new medicines: A novel plant collection strategy towards the discovery of anticancer agents

“…Although no bio-prospecting and isolation work from the Lao PDR has thus far proceeded to the stage of patent filing thereby meriting additional studies (unlike for Vietnam; Zhang et al, 2014Zhang et al, , 2017, through the VietnamLaos ICBG, six medicinal plant taxa from around the country have been evaluated through the compound isolation stage and have been found to contain mostly novel phytochemicals bearing anti-tuberculosis , anti-malarial (He et al, 2005(He et al, , 2006Libman et al, 2008;Ma et al, 2008); and cancer cell cytotoxicity activities (Zhang et al, 2004). With respect to anticancer bio-prospecting from the ICBG field work conducted from 1998 to 2012 in the Lao PDR, at least 34 species yielded 50 extracts with significant activity (IC 50 < 20 μg/ml) in one or more cancer cell lines of a six cell line panel, which notably led to the isolated compounds asparacoside and 3''-methoxy-nyasol (IC 50 > 4 μg/ml, < 10 μg/ml) from Asparagus cochinchinensis (Lour.)…”