Antimicrobial activities of dialysate-elicited and resident human peritoneal macrophages

Peterson, Phillip K.; Gaziano, Emanuel P.; Suh, Hwan J.; Devalon, Melissa L.; Peterson, LC; Keane, William F.

doi:10.1128/iai.49.1.212-218.1985

Cited by 53 publications

(10 citation statements)

References 48 publications

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“…In contrast, peritoneal maerophages from CAPD patients showed an increased antigcn-presenling eapaeity. This tinding suggests functional aetivation of the peritoneal macrophages by the CAPD treatment, as has also been reported in other studies [25,26]. Data from this study indicated that CAPD peritoneal dendritic cells are capable of inducing both a primary and secondary Tcell response at a similar level to control peritoneal DC, In addition, the antigen-presenting capacity ofthe peritoneal maerophages was increased.…”

Section: Discussionsupporting

confidence: 88%

Antigen-presenting capacity of macrophages and dendritic cells in the peritoneal cavity of patients treated with peritoneal dialysis

Betjes

Tuk

Struijk

et al. 1993

Clinical and Experimental Immunology

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SUMMARYIn this study the antigen-presenting capacity of human peritoneal eells and the infiuence of continuous ambulant peritoneal dialysis (C-APD) were studied. On average 6% ofthe peritoneal eells were dendritic eells (DC), with no difference between CAPD and control peritoneal cells. DC were enriched by selecting for non-adherent, Fe reeeptor-negative, low density cells. A typical spot-like CD68 positivity was seen in DC, in contrast to the pancytoplasmic staining pattern in maerophages. Peritoneal DC morphologically and functionally showed features of cells belonging to the DC lineage. Peritoneal DC were superior antigen-pre.senting eells for both allo-antigen, and Candida albicans antigen or purified protein derivative. CAPD peritoneal maerophages were two-to threefold better stimulator cells for allogeneic T cells compared with control maerophages. The level of integrins/adhesins or MHC class 1 or II, as measured semi-quantitatively on the FACS, could not account for this phenomenon. In addition, a double chamber system showed that dialysate-activated macrophages produced soluble factors that could enhance DC-induced allogeneic T cell proliferation. In conclusion, human peritoneal celts contain a relatively high pereentage of classical DC. CAPD treatment does not impair the antigen-presenting capacity of f)eritoneal cells, but instead upregulates the allo-antigen-presenting capacity of peritoneal macrophages-

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Section: Discussionsupporting

confidence: 88%

Antigen-presenting capacity of macrophages and dendritic cells in the peritoneal cavity of patients treated with peritoneal dialysis

Betjes

Tuk

Struijk

et al. 1993

Clinical and Experimental Immunology

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“…This conclusion was indicated by the meager luminol-enhanced CL responses and the inability of catalase, azide, or DMSO to inhibit killing. Although the H202-MPO-halide system is effective in killing B. dermatitidis (6,17), resident peritoneal macrophages are noted for their paucity of MPO (14), and this fact probably accounts for our findings. We speculate that activated macrophages could kill B. dermatitidis by an enhanced ability to discharge (degranulate) lysosomal contents (e.g., cationic proteins [16]) into the B. dermatitidis-macrophage interface.…”

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confidence: 58%

Fungicidal mechanisms of activated macrophages: evidence for nonoxidative mechanisms for killing of Blastomyces dermatitidis

Brummer

Stevens

1987

Infect Immun

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The mechanism(s) by which lymphokine-activated peritoneal macrophages kill Blastomyces dermatitidis was studied. Resident peritoneal macrophages from BALB/cByJ mice, when treated overnight with lymph node cells plus concanavalin A, supernatants from concanavalin A-stimulated spleen cells, or recombinant gamma interferon, were then able to kill a virulent B. dermatitidis isolate (ATCC 26199) (at levels of 25% ± 4%, 28% ± 8%, and 21% ± 5%, respectively). Killing was not significantly decreased or enhanced in the presence of superoxide dismutase (450 U/ml), catalase (20,000 U/mI), dimethyl sulfoxide (300 mM), or azide (1 mM). Viable B. dermatitidis elicited a brisk oxidative burst and superoxide anion production in activated macrophages as measured by lucigenin-enhanced chemiluminescence, e.g., 104 cpm. However, these responses were not significantly different from those of control macrophages. Luminol-enhanced chemiluminescence responses by activated or control macrophages were meager (-102 cpm). These results indicate that activated macrophages kill B. dermatitidis by a mechanism(s) independent of products of the oxidative burst.

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“…CAPD peritoneal cells have features of both immaturity and activation compared to control cells (24). This is particularly true for peritoneal macrophages, which have been reported to be relatively immature cells (6,25), while characteristics such as increased respiratory burst activity and killing of Candida albicans blastophores suggest that they are functionally activated (26,27). We have previously shown that transmigration of monocytes into the peritoneal cavity during the noninfected steady state implies a selective upregulation of functional receptors, preferentially related to adhesion and antigen presentation (19).…”

Section: Discussionmentioning

confidence: 99%

Capd Peritonitis Induces the Production of a Novel Peptide, Daintain/Allograft Inflammatory Factor-1

et al. 2003

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← Objectives To study the occurrence of a novel macrophage-derived peptide, daintain/allograft inflammatory factor-1 (AIF-1), in dialysate from continuous ambulatory peritoneal dialysis (CAPD) patients at commencement and after a follow-up period of therapy and during peritonitis. In addition, we studied peptide production in response to bacterial stimulation of monocytes and macrophages. ← Design Peritoneal fluid and supernatants from cells stimulated with different bacteria were analyzed for daintain/AIF-1. ← Patients and Setting Peritoneal fluid was obtained from 5 patients at commencement of CAPD therapy and during 8 weeks follow-up, and from 14 patients (10 males, 4 females) during CAPD peritonitis and during the noninfected steady state. All patients were admitted to the Karolinska Hospital. A human monocyte cell-line, THP-1 was differentiated to macrophages, and both monocytes and macrophages were stimulated with live and heat-inactivated Escherichia coli, Staphylococcus aureus, and S. epidermidis Cells were also stimulated with interleukin (IL)-1β and interferon gamma (IFNγ). Daintain/AIF-1 was analyzed with radioimmunoassay technique and IL-8 with enzyme immunoassay technique. ← Results An increased production of daintain/AIF-1 was observed in the first spent dialysate in the newly started CAPD patients, with a decrease during the follow-up period ( p < 0.05). During peritonitis, the first spent dialysate revealed significantly higher levels of daintain/AIF-1 (3.9 ng/mL) compared to the noninfected state (0.8 ng/mL), with production normalizing after 9 – 12 days. Bacterial stimulation with E. coli, S. aureus or S. epidermidi sinduced higher daintain/AIF-1 response in monocytes compared to macrophages ( p < 0.05). Live bacteria induced higher production of the peptide compared to heat-inactivated bacteria ( p < 0.05). Interleukin-1β and IFNγ were used to stimulate monocytes and macrophages; however, no daintain/AIF-1 production was found, although increased IL-8 levels were detected. ← Conclusion CAPD peritonitis induces a high and prominent daintain/AIF-1 response. Bacteria are able to induce a response of the peptide from monocytes and macrophages, and it is likely that the virulent parts of the bacteria are heat-labile structures. The early rise in daintain/AIF-1 might be used as a marker of CAPD peritonitis.

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Antimicrobial activities of dialysate-elicited and resident human peritoneal macrophages

Cited by 53 publications

References 48 publications

Antigen-presenting capacity of macrophages and dendritic cells in the peritoneal cavity of patients treated with peritoneal dialysis

Antigen-presenting capacity of macrophages and dendritic cells in the peritoneal cavity of patients treated with peritoneal dialysis

Fungicidal mechanisms of activated macrophages: evidence for nonoxidative mechanisms for killing of Blastomyces dermatitidis

Capd Peritonitis Induces the Production of a Novel Peptide, Daintain/Allograft Inflammatory Factor-1

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