Antimicrobial Activities of Ethanol Extracts of <scp>R</scp>hus javanica and <scp>T</scp>erminalia chebula Against <scp>S</scp>higella Species

Yang, Seung-Kuk; Kim, Hyun-Jung; Oh, Se‐Wook

doi:10.1111/jfs.12102

Cited by 5 publications

(6 citation statements)

References 15 publications

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“…A study conducted inMRSA, revealed that extract of Rhus javanica inhibitedthe bacterial growth by significantlydecreasing the expression ofmecA, sea, agrA and sarA gene [10]. Another studyshowed that, extract showed inhibitory effect against shigellosis causing pathogens,Shigella flexneri and Shigella dysenteriae,like other bacterial pathogens [14].…”

Section: Discussionmentioning

confidence: 97%

In-vitro antibacterial activity of Rhus javanica against multidrug-resistant uro-pathogens

Saud¹,

Paudel²,

Sharma³

et al. 2019

Janaki Med. Coll. J. Med. Sci.

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Background and Objectives: Antibiotic resistance is emerging as a major public health concern. From the ancient time in history, different plants and herbs have been known to have medicinal value. Rhus javanica has been found to show antibacterial activity against pathogenic bacteria. Thus, present study is designed to investigate the prevalence of urinary tract infection causing bacterial pathogens, its antibiogram and effect of Rhus javanica in standard in-vitro condition against multidrug-resistant. Material and Methods: Cross-sectional study was conducted in urinary tract infection suspected patients visiting a clinic in Kalimati, Kathmandu, from July to December 2017. A total of 133 midstream urine samples were collected and cultured in MacConkey agar and Blood agar media and isolates were identified by standard biochemical tests. Antibiotic susceptibility testing was performed according to Clinical and Laboratory Standard Institute (CLSI) guideline. Leaves extract of Rhus javanica was obtained by maceration using 50.0% methanol and susceptibility testing was performed by using cork borer method in Mueller-Hinton agar. Results: Out of total 133 samples, 35.3%showed significant bacterial growth (i.e. 105 cfu/ml) on agar plate. The most predominant organism was Escherichia coli 17.3%, followed by Enterococcus faecalis 6.8%, Klebsiella pneumoniae 3.8%, Pseudomonas aeruginosa 3.0%, Proteus vulgaris 2.3%, and Staphylococcus aureus 2.3%. Out of 47 isolates, 53.2% were Multi Drug Resistant (MDR). Various concentrations of the crude extract used (25, 50, 75 and 100 mg/ml) showed susceptibility to all the bacterial pathogens isolated, with the diameter of zone of inhibition ranging from 12 to 28 mm. Conclusion: Methanolic extract of Rhus javanica showed antibacterial activity against multidrug-resistant isolates of bacterial uropathogens in standard in-vitro condition.

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Section: Discussionmentioning

confidence: 97%

In-vitro antibacterial activity of Rhus javanica against multidrug-resistant uro-pathogens

Saud¹,

Paudel²,

Sharma³

et al. 2019

Janaki Med. Coll. J. Med. Sci.

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show abstract

“…A study conducted in MRSA, revealed that extract of Rhus javanica inhibited the bacterial growth by significantly decreasing the expression of mecA, sea, agrA and sarA gene [10]. Another study showed that, extract showed inhibitory effect against shigellosis causing pathogens,Shigella flexneri and Shigella dysenteriae, like other bacterial pathogens [14].…”

Section: Discussionmentioning

confidence: 99%

In-vitro Antibacterial activity of Rhus javanica Against Multidrug-Resistant Uropathogens

2020

AJUN

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Background and objective: Antibiotic resistance is emerging as a major public health concern. From the ancient time in history, different plants and herbs have been known to have medicinal value. Rhus javanica has been found to show antibacterial activity against pathogenic bacteria. Thus, present study is designed to investigate the prevalence of urinary tract infection causing bacterial pathogens, its antibiogram and effect of Rhus javanica in standard in-vitro condition against multidrug-resistant. Materials and Methods: Cross-sectional study was conducted in urinary tract infection suspected patients visiting a clinic in Kalimati, Kathmandu, from August to October 2019. A total of 133 midstream urine samples were collected and cultured in MacConkey agar and Blood agar media and isolates were identified by standard biochemical tests. Antibiotic susceptibility testing was performed according to Clinical and Laboratory Standard Institute (CLSI) guideline. Leaves extract of Rhus javanica was obtained by maceration using 50.0% methanol andsusceptibility testing was performed by using cork borer method in Mueller-Hinton agar. Results: Out of total 133 samples, 35.3%showed significant bacterial growth (i.e. 105 cfu/ml) on agar plate. The most predominant organism was Escherichia coli 17.3%, followed by Enterococcus faecalis 6.8%, Klebsiella pneumoniae 3.8%, Pseudomonas aeruginosa 3.0%, Proteus vulgaris 2.3%, and Staphylococcus aureus 2.3%. Out of 47 isolates, 53.2% were Multi Drug Resistant (MDR). Various concentrations of the crude extract used (25, 50, 75 and 100 mg/ml) showed susceptibility to all the bacterial pathogens isolated, with the diameter of zone of inhibition ranging from 12 to 28 mm. Conclusion: Methanolic extract of Rhus javanica showed antibacterial activity against multidrug-resistant isolates of bacterial uropathogens in standard in-vitro condition.

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“…has been reported from different parts (root, stem, gallarhois) of R. chinensis. 9,[15][16] Recently, antibacterial activity of R. chinensis against methicillin-resistant Staphylococcus aureus, 6 Shigella species 17 and Streptococcus iniae 18 is reported. R. chinensis extract was identified as the most effective anti-inflammatory and anti-photoaging agent.…”

Section: Introductionmentioning

confidence: 99%

“…Antibacterial activity of the fruits has never been reported though the leaves had displayed antimicrobial activity. 6,[17][18] The phytochemicals present in the plant material were also investigated by chemical tests and GC-MS analysis to identify the biologically active phytochemicals.…”

Section: Introductionmentioning

confidence: 99%

“…You et al have reported antimicrobial activity of the ethanolic extract of R. chinensis leaf against methicillin-resistant S. aureus, and mentioned its bactericidal effect on the bacterial strain 6. Yang et al have also reported antimicrobial activity of the ethanolic extract of R. chinensis against S. dysenteriae;17 however, to the best of our knowledge, antimicrobial activity of the fruits of R. chinensis is not evaluated yet.MIC and MBC values obtained are tabulated inTable 4. The 70% methanolic extract displayed bactericidal effect on S. aureus, E. faecalis, E. coli and P. aeruginosa while it was bacteriostatic to B. subtilis, K. pneumoniae and S. dysenteriae.…”

mentioning

confidence: 99%

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Ethnomedicinal Knowledge Verification for the Antidiarrheal and Antioxidant Effects of Rhus chinensis Mill. Fruits with Identification of Thirty Constituents

Nemkul¹,

Bajracharya²,

Maeda³

et al. 2021

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Background: Ethnobotanical survey in the rural villages in Nepal revealed that the fruits of Rhus chinensis Mill. have been using for the treatment of diarrhea and dysentery. Objective: To evaluate antimicrobial and antioxidant effects, and identification of chemical constituents in the fruits of R. chinensis. Materials and Methods: Phytochemical screening was performed on the hexane and 70% methanolic extracts of the sample followed by gas chromatographymass spectrometry (GC-MS). Total phenolic content (TPC) was estimated using Folin-Ciocalteu method. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical and hydrogen peroxide scavenging assays were used to evaluate the antioxidant capacity. Antibacterial effect was estabilished by the Agar well diffusion assay. Results: A total of 30 compounds belonging to phenolics, anhydrides, aldehydes, fatty acids and hydrocarbons were identified in the extracts. The TPC value of 123.52±1.29 mg GAE/g dry extract was estimated. IC 50 value of 135.54±0.82 µg/mL was calculated in DPPH free radical scavenging assay. Scavenging of 42.69±0.1% DPPH free radical and 63.20±1.48% hydrogen peroxide at 100 µg/mL concentration of 70% methanolic extract were estimated. The maximum zone of inhibition (ZOI) observed was 23.00±0.57 mm against Escherichia coli at loading dose of 5 mg of the extract. Conclusion: All together 30 compounds were identified in the fruits. The extracts efficiently inhibited the growth of E. coli and Shigella dysenteriae verifying the rural knowledge. At the same time, the extracts displayed efficient antioxidant activity. The phytochemicals identified were responsible for these activities.

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Antimicrobial Activities of Ethanol Extracts of Rhus javanica and Terminalia chebula Against Shigella Species

Cited by 5 publications

References 15 publications

In-vitro antibacterial activity of Rhus javanica against multidrug-resistant uro-pathogens

In-vitro antibacterial activity of Rhus javanica against multidrug-resistant uro-pathogens

In-vitro Antibacterial activity of Rhus javanica Against Multidrug-Resistant Uropathogens

Ethnomedicinal Knowledge Verification for the Antidiarrheal and Antioxidant Effects of Rhus chinensis Mill. Fruits with Identification of Thirty Constituents

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