Antimicrobial Activities of Lectins Extracted from Some Cultivars of Phaseolus vulgaris Seeds

El-S, Einas Hamed; El-A, Magda Mahmoud Ibrahim; S, Mervat Mounir

doi:10.4172/1948-5948.1000352

Cited by 7 publications

(7 citation statements)

References 41 publications

(34 reference statements)

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“…Lectins are proteins or glycoproteins that have at least one binding site without catalytic function or immunological characteristics [105]. Hamed [106] reported separation of lectins from five cultivars of Phaseolus and evaluated their antimicrobial activity against Gram-negative bacteria Pseudomonas aeruginosa and Klebsiella pneumoniae, and Gram-positive bacteria Staphylococcus aureus, the mode of action of lectins was potential agglutination of bacterial cells which was confirmed by scanning electron microscope (SEM) [107] [108] [109]. Lectins in Phaseolus vulgaris were found to agglutinate and aggregate bacterial cells together [106].…”

Section: Mode Of Action Of Plant Secondary Metabolitesmentioning

confidence: 99%

“…Hamed [106] reported separation of lectins from five cultivars of Phaseolus and evaluated their antimicrobial activity against Gram-negative bacteria Pseudomonas aeruginosa and Klebsiella pneumoniae, and Gram-positive bacteria Staphylococcus aureus, the mode of action of lectins was potential agglutination of bacterial cells which was confirmed by scanning electron microscope (SEM) [107] [108] [109]. Lectins in Phaseolus vulgaris were found to agglutinate and aggregate bacterial cells together [106]. Berger [110] reported that glycerol ethers and related compounds present in dimethyl ether extract may be responsible for the antimicrobial activity due to respiratory collapse.…”

Section: Mode Of Action Of Plant Secondary Metabolitesmentioning

confidence: 99%

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Phytochemicals and Their Antimicrobial Activity: An Update on Their Mode of Action

Pammi¹,

Giri²

2021

IJCEMR

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Introduction: Plants have been considered as potential alternative for antimicrobial antibiotics owing to vast reserve of secondary metabolites. Last few decades have experienced an upsurge in demand and delivery of herbal products for health benefits as plant derived bioactive compounds have capability to interact with a wide variety of targets. The main focus of this review was to congregate vital information related to imperative bioactive components of medicinal plants having therapeutic potential with particular reference to antimicrobial activity against pathogenic bacteria. Our study intended to comprehend the mode of action of these phytopharmaceuticals for their scientific validation and drug development. Methods: A narrative review of papers reporting antimicrobial activity of medicinal plants against pathogenic bacteria, identification of the prospective bioactive compounds with antimicrobial potential and understanding their mechanism of action has been envisaged. Results: This review includes anthology of recent published information on phytoconstituents of various medicinal plants belonging to different families around the globe, tested and validated against various pathogenic bacteria. This study unveils the treasure of antimicrobial potential of plant species, which is also an appraisal of information related to mechanism of action of these phytochemicals required for their antimicrobial activity. Conclusions: There is resurgence in use of herbal medicines and identification of pharmacologically active compounds with their scientific validation. It will play a pivotal role in perpetuating and promoting wider usages of drugs based on plant extracts. A missing link for establishment of phytocompounds as an alternative drug requires understanding their mode of action and exploitation.

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Section: Mode Of Action Of Plant Secondary Metabolitesmentioning

confidence: 99%

Section: Mode Of Action Of Plant Secondary Metabolitesmentioning

confidence: 99%

Phytochemicals and Their Antimicrobial Activity: An Update on Their Mode of Action

Pammi¹,

Giri²

2021

IJCEMR

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“…One promising avenue for finding novel antibacterial remedies lies in the exploration of natural products that are commonly consumed. Antimicrobial peptides (AMPs) have emerged as a focal point of study, and applications in both agriculture and medicine have been touted [ 10 ]. AMPs are a widespread class of molecules found in diverse organisms, ranging from microbes to animals, and are key components of natural defense systems against pathogens and pests.…”

Section: Introductionmentioning

confidence: 99%

“…Rich in amino acids, red kidney beans offer a cost-effective source of dietary protein, typically comprising 20% to 30% protein content based on dry weight. Previous investigations on peptides isolated from related species have demonstrated various biological activities [ 10 , 13 ]. Antimicrobial protein hydrolysate was isolated from P. vulgaris, which is active against some Gram-positive and Gram-negative bacteria [ 14 ], setting a foundation for exploring the antimicrobial potential of crude peptides from red kidney beans.…”

Section: Introductionmentioning

confidence: 99%

Peptide Extract from Red Kidney Beans, Phaseolus vulgaris (Fabaceae), Shows Promising Antimicrobial, Antibiofilm, and Quorum Sensing Inhibitory Effects

Tetteh,

Wereko Brobbey,

Osei

et al. 2024

Biochemistry Research International

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The rapid spread of multidrug-resistant bacteria has led to an increased risk of infectious diseases. Pseudomonas aeruginosa, in particular, poses a significant obstacle due to its propensity to rapidly acquire resistance to conventional antibiotics. This has resulted in an urgent need for the development of new classes of antibiotics that do not induce resistance. Antimicrobial peptides (AMPs) have been studied as potential small-molecule antibiotics due to their unique mode of action. In this study, peptides were extracted from the seeds of Phaseolus vulgaris (Fabaceae), and the antimicrobial activities of the extract were evaluated using microbroth dilution against five different microorganisms. The extract showed antimicrobial activity against all tested organisms with minimum inhibitory concentrations (MIC) of 2.5 mg/mL, except for Candida albicans and Pseudomonas aeruginosa, which had MICs of 1.25 mg/mL. The extract was also bacteriostatic for all tested organisms. The crude peptide extract from Phaseolus vulgaris was further studied for its antibiofilm activity against Pseudomonas aeruginosa, a common nosocomial pathogen associated with biofilm formation. The extract showed good antibiofilm activity at 1/2 MIC. The extract also inhibited the expression of pyocyanin and pyoverdine (virulence factors of P. aeruginosa whose expression is mediated by quorum sensing) by 82% and 66%, respectively. These results suggest that the peptide mix from Phaseolus vulgaris may inhibit biofilm formation and virulence factor expression by interfering with cell-to-cell communication in Pseudomonas aeruginosa. The ability of the extract to inhibit the growth and biofilm formation of all tested organisms indicates its potential as an antimicrobial agent that could be further studied for drug discovery.

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“…PHA generally consists of two polypeptide subunits known as PHA-L and PHA-E, which exhibits preferential binding characteristics to red blood cells and white blood cells, respectively, attributed to the presence of specific glycans chains attached to the polypeptides’ surfaces. The PHA-L subunit has a relative molecular weight of about 30 kDa, while PHA-E subunit weights approximately 34 kDa (Leavitt et al, 1977; Einas Hamed et al, 2017). The natural PHAs are tetramers randomly composed of five isomeric forms, including E4, E3L1, E2L2, E1L3, and L4 (He et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

A precise IDMS-based method for absolute quantification of phytohemagglutinin, a major antinutritional component in common bean

Li,

Chu,

Ning

et al. 2023

Preprint

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Phytohemagglutinin (PHA), a natural tetramer comprising PHA-E and PHA-L subunits that preferentially bind to red and white blood cells, respectively, constitutes a significant antinutritional and allergenic factor in common bean seeds. The accurate measurement of PHA content is a prerequisite for ensuring food safety inspections and facilitating genetic improvements in common bean cultivars with reduced PHA levels. Currently, mainstream methods for PHA quantification involve hemagglutination assays and immunodetection, but these methods often require fresh animal blood and lack specificity and accuracy. In this study, we present a novel LC-MS/MS-based method for PHA quantification, leveraging the advantages of isotope dilution mass spectrometry (IDMS). Two signature peptides each for PHA-E and PHA-L, along with a common signature peptide, were identified and employed for quantification, allowing differentiation between PHA-E and PHA-L subunits. The incorporation of amino acid analysis-isotope dilution mass spectrometry (AAA-IDMS) enabled precise determination of the synthetic signature peptides’ purity during measurement, enhancing metrological accuracy. In addition, the TCA-acetone protocol was established as the optimized method for total protein extraction from dry bean seeds. Quantitative analysis of PHA-E and PHA-L subunits in six common bean varieties using the developed method demonstrated excellent linearity (r > 0.999), sensitivity (limit of detection and quantitation as low as 2.32 ng/mg and 7.73 ng/mg, respectively), recovery (94.18-104.47%), and repeatability (relative standard deviation < 3.45%). This method has the potential to serve as a standard for measuring PHA contents in common beans and other agricultural products containing PHA.

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Antimicrobial Activities of Lectins Extracted from Some Cultivars of Phaseolus vulgaris Seeds

Cited by 7 publications

References 41 publications

Phytochemicals and Their Antimicrobial Activity: An Update on Their Mode of Action

Phytochemicals and Their Antimicrobial Activity: An Update on Their Mode of Action

Peptide Extract from Red Kidney Beans, Phaseolus vulgaris (Fabaceae), Shows Promising Antimicrobial, Antibiofilm, and Quorum Sensing Inhibitory Effects

A precise IDMS-based method for absolute quantification of phytohemagglutinin, a major antinutritional component in common bean

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