Antimicrobial activity of 5-alkyl-3H-furanones and their sulfur analogs

Morozova, N. A.; Sedavkina, V. A.; Egorova, A. Yu.; Sorokin, N. N.; Kulikova, L. K.

doi:10.1007/bf00764465

Cited by 3 publications

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“…These included certain compounds that have potential antibacterial activity. For example, aromatic aliphatic esters of furanone and derivatives, and tetrahydrofuran esters have been previously shown to be antibacterial [245,246].…”

Section: Maggot Extracts and Its Combination With Ciprofloxacinmentioning

confidence: 99%

Investigation of combinatory drug mix on cell behavior

Arora¹

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Real-time combinatory drug responses on biological cells have become important for drug screenings with growing threat of diseases. Though rapid progress has been made in the field of biotechnology and pharmacy, the need to understand the effects of drug combinations, such as antibiotics and herbal medicines, on cells have become essential.Drug combinations are used to accelerate and/or increase the efficacy of a treatment. In case of infectious diseases, however, the predicaments of drug resistance prevail in the contemporary scenario. Drug combinations, in these cases, are mainly used to overcome such resistance by the disease causing pathogens.The present project created a unique system that can simultaneously investigate cell behavior exposed to a combinatory mixture of drugs. The system was aimed to conduct novel studies of combinatory drug performance on various cell types in vitro. Protocols for handling different cell types that require different culture conditions were established.The system consisted of a MEMS-based microchannel device and a controlled incubator chamber to accommodate the device that provided an environment for cell sustenance.Together with the control and electronic circuitry, the drug and cell injection systems, the setup was coined as the Combinatory Drug Screening System or CDSS. The system allowed monitoring and tracking of cells inoculated in the culturing chambers of the micro-device, under influence of drug combinations. The combinatory drug response, physiological behavioral changes, such as cell count or optical density, and/or tracking of cell mutations, could be recorded from the system using spectroscopy or microscopy.As a proof-of-concept, different cell types were initially tested in the system. Mammalian adhesion-type cells and bacterial suspension-type cells were used to establish initial results that closely matched the growth curves of the conventional cell culturing techniques. Subsequently, the system was used to investigate the effects antibiotic, ciprofloxacin in combination with four antibacterial phytochemicals and maggot excretions/secretions (ES), ABSTRACT INVESTIGATION OF COMBINATORY DRUG MIX ON CELL BEHAVIOR vii on Staphylococcus aureus. S. aureus is one of the most common nosocomial pathogens, causing diseases ranging from superficial dermatological lesions to systemic and life threatening infections such as endocarditis. Due to its rapid adaptive behavior under antibiotic influence, its genome has been termed as the "super-genome" and itself as the "super-bug". A particular strain of S. aureus, methecillin-resistant S. aureus or MRSA is known to be resistant to almost all available antibiotics.The antibiotic-phytochemical combination investigated on S. aureus using the CDSS, was shown to delay the occurrence of resistance, which was otherwise encountered after 72 hours of antibiotic exposure. The phytochemicals, therefore, helped to potentiate the activity of ciprofloxacin, when their activity alone was negligible.The maggot extracts investigated for the f...

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Section: Maggot Extracts and Its Combination With Ciprofloxacinmentioning

confidence: 99%