Antimicrobial Activity of <i>Cannabis sativa</i> L.

Abstract

The oil of the seeds, petroleum ether and methanol extracts of the whole plant of Cannabis sativa belonging to the family Cannabinaceae were screened for their antimicrobial activity against two Gram positive organisms (<i>Bacillus subtilis</i>, <i>Staphylococcus aureus</i>), two Gram negative organisms (<i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>) and two fungi namely <i>Aspergillus niger</i> and <i>Candida albicans</i> using the cup plate agar diffusion method. The oil of the seeds of Cannabis sativa exerted pronounced antibacterial activity (21 - 28 mm) against <i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i>, moderate activity (15 mm) against <i>Escherichia coli</i> and high activity (16 mm) against <i>Pseudomonas aeruginosa</i> and inactive against the two fungi tested. The petroleum ether extract of the whole plant exhibited pronounced antibacterial activity (23 - 28 mm) against both <i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i> organisms, high activity (16 mm) against <i>Escherichia coli</i> and inactive against <i>Pseudomonas aeruginosa</i> and both fungi. The methanol extract of the whole plant showed also pronounced antibacterial activity (29 mm) against <i>Bacillus subtilis</i>, low activity (12 mm) against <i>Staphylococcus aureus</i> and high activity (16 - 18 mm) against both Gram negative organisms, inactive against Aspergillus niger and low activity (13 mm) against <i>Candida albicans</i>. The minimum inhibitory concentrations of <i>Cannabis sativa</i> methanol extracts of the seeds and the whole plant against the standard organisms were determined using the agar plate dilution method. The standard organisms were tested against reference antibacterial and antifungal drugs and the results were compared with the activity of the extracts

Isolation of Nine Microorganisms from Rotten <i>Dioscorea rotundata</i> (White Yam) and Antimicrobial Sensitivity Test with Five Plant Extracts

“…The organism Serratia marcescens was resistant to the extracts, showing only mild sensitivity with Daniella oliveri, Ceiba pentandra, Jatropha tanjorensis extracts at undiluted concentrations only. This finding is in agreement with earlier report by [29] [30].…”

Cannabinoids and Terpenes as an Antibacterial and Antibiofouling Promotor for PES Water Filtration Membranes

“…This considerable bacterial decline in the CT-PES hybrid membranes when compared to the pure PES membranes was attributable to the synergistic antibacterial activity of cannabinoids and terpenes ( Figure 6), which has not only been reported in the present research, but also reported previously [39,40]. In addition to this, the antibacterial properties of the CT PES membranes could also be attributable to a rise in hydrophilicity, which may lead to lower the attachment of Gram-positive and Gram-negative bacteria on the membrane surface [41].…”

Antimicrobial Potential of Three Common Weeds of Kurukshetra: An in vitro Study

“…The classical cannabinoids are structurally related to Tetrahydrocannabinol cannabinoids, a unique group of secondary metabolites found in the plant responsible for pharmacological effects. Currently, there are three general types of cannabinoids; phytocannabinoids occur uniquely in the cannabis plant; endogenous cannabinoids are produced in the bodies of humans and animals and synthetic cannabinoids which are similar compounds produced in the laboratory (Lambert and Fowler, 2005;Ali et al, 2012). Res.…”