Antimicrobial Activity of Antibiotic-treated Amniotic Membrane

Mencucci, Rita; Menchini, Ugo; Dei, Rosanna

doi:10.1097/01.ico.0000214207.06952.23

Cited by 34 publications

(27 citation statements)

References 31 publications

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“…Clinically, we were assured by the findings that AMT itself does not impede penetration of topical antibiotics,2 and that AM soaked with antibiotics actually prolong the bactericidal effect,28 thus allowing one to use less but avoiding antimicrobial's potential cytotoxic effect. Although we noted that insertion of ProKera as early as 96 hours after intensive fortified antibiotic treatment in patient 1 after the pathogen was identified could deliver AM's desirable actions with success, our small series of 3 patients was still too few to determine whether this is an optimal timing for all infectious keratitis.…”

Section: Discussionmentioning

confidence: 99%

Sutureless Amniotic Membrane Transplantation for Severe Bacterial Keratitis

Sheha

Liang

et al. 2009

Cornea

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Purpose-To evaluate the efficacy of early sutureless amniotic membrane transplantation in the management of severe bacterial keratitis to reduce pain, inflammation, and haze, and to promote healing.Method-A noncomparative case series including 3 eyes of 3 consecutive patients with severe bacterial keratitis exhibiting persistent epithelial defect/ulcer, more than 5 mm in diameter, located within 3mm from the visual axis with infiltration occupying more than 50% of the corneal thickness. They were retrospectively reviewed following early (ie, within 96 hours) sutureless amniotic membrane transplantation via ProKera together with selective topical antibiotics and preservativefree steroid. Pain relief, inflammation, haze, and corneal epithelial healing were monitored.Results-ProKera was inserted once in 1 eye and twice in the other 2 eyes. Pain was significantly relieved and inflammation was markedly reduced in all cases. The corneal epithelial defect and stromal ulceration rapidly healed while visual acuity improved in 2 of the 3 eyes.Conclusion-Temporary sutureless amniotic membrane transplantation via ProKera allows easy insertion and replacement of the membrane in the office, as well as early intervention to promote epithelialization, reduce pain, haze and inflammation in cases with severe bacterial keratitis. This result justifies large series controlled studies in the future.Keywords amniotic membrane; bacterial keratitis; sutureless; wound healing Microbial keratitis (caused by bacteria, fungi, viruses, or parasites) is a serious ocular infection resulting in persistent epithelial defect/ulcer at the acute phase but corneal scarring and a permanent loss of vision at the chronic phase. The principal therapeutic goals for infectious keratitis are to eliminate the pathogens and to prevent irreversible corneal structural damage.

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Section: Discussionmentioning

confidence: 99%

Sutureless Amniotic Membrane Transplantation for Severe Bacterial Keratitis

Sheha

Liang

et al. 2009

Cornea

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“…The possibility to use AM to deliver drugs has been first suggested by Kim et al 14 and Heilinghaus et al 26 and then by Mencucci et al 19 and Gicquel et al 13. As expected, AM can absorb ACV or TFU; antiviral uptake occurs quite rapidly.…”

Section: Discussionmentioning

confidence: 88%

Inhibition of viral replication in vitro by antiviral-treated amniotic membrane. Possible use of amniotic membrane as drug-delivering tool

Mencucci

Paladini

Menchini

et al. 2010

British Journal of Ophthalmology

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“…In intractable infections, either gluing or suturing AM to the affected area has been shown to induce a favourable response to ongoing treatment. The exact mechanism of action of AM is unknown, but it is believed that the AM acts as a reservoir of the instilled medication and concentrates drug delivery to the site of infection [38,39] ( fig. 16 d-f).…”

Section: Surgical Options For Infective Keratitismentioning

confidence: 99%

The Ocular Surface: Functional Anatomy, Medical and Surgical Management

Dua¹,

Said²

2015

Cornea

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The ocular surface (OS) is a functional unit comprising the eyelids and blink reflexes, tear film and tear glands, the conjunctival mucus membrane and substantia propria, and the corneal epithelium and Bowman's zone. Its health is crucial for vision. The OS can be affected by a variety of degenerative, inflammatory and neoplastic diseases, as well as by trauma. Significant amongst these are dry eye disease, microbial infections and immune-mediated inflammatory disorders ranging from allergic conjunctivitis to destructive conditions such as ocular cicatricial pemphigoid and chemical burns. Several of these can lead to persistent epithelial defects with risk of perforation. Management principles include a thorough history and clinical evaluation of signs and symptoms to establish the diagnosis and determine the extent of disease. Treatment is tailored to the specific condition and ranges from lubricant drops to antimicrobial agents and anti-inflammatory drugs such as steroids and steroid-sparing agents. A variety of surgical procedures are often required both as adjunctive or primary interventions. These can be simple outpatient procedures such as punctal plugs and tarsorrhaphy or complex procedures such as amniotic membrane grafts, keratoplasty and limbal stem cell transplantation. In end-stage diseases where the OS is severely compromised and dry, keratoprosthesis remains the only hope to restore some sight. The Ocular SurfaceThe term 'ocular surface' (OS) was coined by Richard Thoft [1] to include the entire mucus membrane lining from the lid margins across the posterior surfaces of the eyelids, the superior, inferior, medial and lateral fornices, the eye ball, the limbus and the corneal surface together with the tear film. As an anatomical unit, the OS includes the entire conjunctival, the limbal and corneal epithelium. However, as a functional unit, the OS includes the eyelids and their movements together with the glands that secrete the various components of the tear film. As far as the depth of the OS is concerned, different authors have been fairly liberal in limiting it to the epithelium or extending it to the entire thickness of the conjunctiva and all layers of the cornea. Functional AnatomyThe key player at the OS is the transparent cornea, which interfaces with light rays as they traverse to the retina. The other elements of the OS serve to maintain a healthy environment in which the cornea can retain its transparency and facilitate vision. Despite its very organized structure, Güell JL (ed): Cornea.

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Antimicrobial Activity of Antibiotic-treated Amniotic Membrane

Abstract: Our preliminary in vitro data show that amniotic membrane can absorb the antibiotic netilmicin and in the future may be used to deliver antibiotics, as reported for collagen shields and other medical prosthetic devices.

Cited by 34 publications

References 31 publications

Sutureless Amniotic Membrane Transplantation for Severe Bacterial Keratitis

Sutureless Amniotic Membrane Transplantation for Severe Bacterial Keratitis

Inhibition of viral replication in vitro by antiviral-treated amniotic membrane. Possible use of amniotic membrane as drug-delivering tool

The Ocular Surface: Functional Anatomy, Medical and Surgical Management

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