Antimicrobial activity of copper surfaces against biofilm formation by Salmonella Enteritidis and its potential application in the poultry industry

Pontin, Karine Patrin; Borges, Karen Apellanis; Furian, Thales Quedi; Carvalho, Daiane; Wilsmann, Daiane Elisa; Cardoso, Henrique Ribeiro Piaggio; Alves, Annelise Kopp; Chitolina, Gabriela Zottis; Salle, Carlos Tadeu Pippi; Moraes, Hamilton Luiz de Souza; Nascimento, Vladimir Pinheiro do

doi:10.1016/j.fm.2020.103645

Cited by 30 publications

(19 citation statements)

References 39 publications

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“…S. aureus and S. enteritidis can form biofilms on solid surfaces [4,5]. A biofilm comprises strongly enclosed microorganisms that cling to a surface and/or to each other, generating complex actionable circumstances [6][7][8]. Biofilms are found on food contact structures, such as band carriers, vessels, and pipes [9].…”

Section: Introductionmentioning

confidence: 99%

Synergistic Antibiofilm Effects of Ultrasound and Phenyllactic Acid against Staphylococcus aureus and Salmonella enteritidis

Zhang

Wang

Sun

et al. 2021

Foods

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This study evaluated the effect of the combination of ultrasound and phenyllactic acid (PLA) on inactivating Staphylococcus aureus and Salmonella Enteritidis biofilm cells and determined the possible antibiofilm mechanism. S. aureus and S. Enteritidis biofilm cells were separately treated with ultrasound (US, 270 W), phenyllactic acid (PLA, 0.5% and 1%), and their combination (US + 0.5% PLA, and US + 1% PLA) for 5, 10, 20, 30, and 60 min. Biofilm inactivation, polysaccharide, and respiratory chain dehydrogenase assays were conducted. US and PLA had a synergistic effect on inactivating bacterial cells in S. aureus and S. Enteritidis biofilms. The combination of US and PLA significantly decreased the contents of soluble and insoluble polysaccharides and the activity of respiratory chain dehydrogenase in the biofilm cells compared to the single treatment. Confocal laser scanning microscopy, scanning electron microscopy, and intracellular adenosine-triphosphate (ATP) analyses indicated that the combination of US and PLA seriously destroyed the cell membrane integrity of the S. aureus and S. Enteritidis biofilms and caused the leakage of intracellular ATP. These findings demonstrated the synergistic antibiofilm effect of US combined with PLA and offered a research basis for its application in the food industry.

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Section: Introductionmentioning

confidence: 99%

Synergistic Antibiofilm Effects of Ultrasound and Phenyllactic Acid against Staphylococcus aureus and Salmonella enteritidis

Zhang

Wang

Sun

et al. 2021

Foods

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“…Although copper is important to maintain physiological functions, it also exhibits antimicrobial activity against bacterial infection as intracellular accumulation of copper may cause protein damage and cell injury in bacteria ( Dalecki et al., 2017 ; Pontin et al., 2021 ). But, excessive use of copper regulates copper tolerance of pathogenic bacteria against its toxicity, enhancing the virulence of these pathogens ( Singh et al., 1986 ; Medardus et al., 2014 ; Parsons et al., 2017 , 2020 ; Arai et al., 2019 ).…”

Section: Coppermentioning

confidence: 99%

Trace metals and animal health: Interplay of the gut microbiota with iron, manganese, zinc, and copper

2021

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Metals such as iron, manganese, copper, and zinc are recognized as essential trace elements. These trace metals play critical roles in development, growth, and metabolism, participating in various metabolic processes by acting as cofactors of enzymes or providing structural support to proteins. Deficiency or toxicity of these metals can impact human and animal health, giving rise to a number of metabolic and neurological disorders. Proper breakdown, absorption, and elimination of these trace metals is a tightly regulated process that requires crosstalk between the host and these micronutrients. The gut is a complex system that serves as the interface between these components, but other factors that contribute to this delicate interaction are not well understood. The gut is home to trillions of microorganisms and microbial genes (the gut microbiome) that can regulate the metabolism and transport of micronutrients and contribute to the bioavailability of trace metals through their assimilation from food sources or by competing with the host. Furthermore, deficiency or toxicity of these metals can modulate the gut microenvironment, including microbiota, nutrient availability, stress, and immunity. Thus, understanding the role of the gut microbiota in the metabolism of manganese, iron, copper, and zinc, as well as in heavy metal deficiencies and toxicities, and vice versa, may provide insight into developing improved or alternative therapeutic strategies to address emerging health concerns. This review describes the current understanding of how the gut microbiome and trace metals interact and affect host health, particularly in pigs.

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“…Cu is an important trace element (recommended daily allowance of 2.0-3.0 mg/day for humans) for several enzymes involved in antioxidant response, membrane and DNA integrity, and adenosine triphosphate production [25,26]. Cu accumulation in bacteria can cause protein damage and cell injury [26,27], and high levels of Cu exposure can cause toxicity and the development of copper resistance in a variety of pathogenic bacteria [28,29]. Concentration of Cu at upstream, midstream, and downstream of the Miri River, at 0.5 m depth and 1.5 m depth, and variation in concentration at low tide and high tide conditions are presented in Figure 3.…”

Section: Copper (Cu)mentioning

confidence: 99%

Level and distribution of heavy metals in Miri River, Malaysia

Maharjan

Wong

Rubiyatno

2021

Trop. Aqua. Soil Pollut.

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Heavy metal pollution in water resources has become a serious and hazardous environmental problem all over the world because of its non-biodegradability, emanating from multiple sources, easy accumulation, and biological toxicity. This research was carried out to study the level and distribution of heavy metals at different sampling locations (upstream, midstream, and downstream), at different depths (0.5 m and 1.5 m from surface water level), and during low tide and high tide conditions in the Miri River of Miri City in Malaysia. The river water samples were collected and analyzed for Ca, Mg, Cu, Fe, Mn, Ni, Pb, and Zn by flame atomic absorption spectrophotometer. The concentration of Ca was found to be the highest in the Miri River, followed by Mg and Fe, and with traces of Cu, Mn, Ni, Pb, and Zn. An increase in the concentration of heavy metals, such as Cu, Mg, and Ni, was observed while flowing from upstream to downstream of the Miri River. Concentrations of heavy metals, such as Ca, Mg, Cu, and Zn, were clearly lower at 1.5 m depth than at 0.5 m depth. High tides in the river decrease the concentration of heavy metals, such as Ca, Cu, Mn, and Ni, compared to low tides. From this research, it gets clear that using the Miri River water for domestic and recreational purposes, washing, and fishing is detrimental to human health and the environment.

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Antimicrobial activity of copper surfaces against biofilm formation by Salmonella Enteritidis and its potential application in the poultry industry

Cited by 30 publications

References 39 publications

Synergistic Antibiofilm Effects of Ultrasound and Phenyllactic Acid against Staphylococcus aureus and Salmonella enteritidis

Synergistic Antibiofilm Effects of Ultrasound and Phenyllactic Acid against Staphylococcus aureus and Salmonella enteritidis

Trace metals and animal health: Interplay of the gut microbiota with iron, manganese, zinc, and copper

Level and distribution of heavy metals in Miri River, Malaysia

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