Antimicrobial Activity of Crude Extracts of Some Plant Leaves

Obeidat, Maher; Shatnawi, Mohamad; Al-alawi, Mohammad S.; Al-Zu`bi, Enas; Al-Dmoor, Hanee M.; Al-Qudah, Maisa M.; El-Qudah, Jafar M.; Otri, Ismael

doi:10.3923/jm.2012.59.67

Cited by 113 publications

(80 citation statements)

References 12 publications

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“…Cotton swab was then used to inoculate the test tube suspension onto the surface of Muller Hinton agar plate and the plate was allowed to dry. Sterilized Whatman paper disks (6 mm in diameter) were treated with desired concentration of previously prepared ethanolic solution of extract using a micropipette and dried in air under aseptic condition and placed at equidistance in a circle on the seeded plate. The concentration of the extract used was 2 mg/disc.…”

Section: Antimicrobial Screening By Disk Diffusion Techniquementioning

confidence: 99%

“…This situation forced scientists to search for new antimicrobial agents from various sources like medicinal plants which are good sources of novel antimicrobial agents [6] . Leucas aspera (L. aspera), belonging to the family of Labiatae, is a common aromatic herb known as Darkolos or Dandokolos in Bangladesh and found as weed in Asia-temperate, Africa and Asia-tropical countries.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant, antibacterial and cytotoxic effects of the phytochemicals of whole Leucas aspera extract

Rahman

Islam

2013

Asian Pacific Journal of Tropical Biomedicine

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Section: Antimicrobial Screening By Disk Diffusion Techniquementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antioxidant, antibacterial and cytotoxic effects of the phytochemicals of whole Leucas aspera extract

Rahman

Islam

2013

Asian Pacific Journal of Tropical Biomedicine

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“…The antifungal (anticandidiasis) activities of the methanol extract of T. madagascariense (0.098 -12.5 mg/ml) were higher than that of the methanol extract of Lupinus varius (32 mg/ml) against Candida albicans. The antifungal (anticandidiasis) activities of the extract of T. madagascariense producing inhibition zones ranging between 17 and 25 ± 1.0 mm was higher than that of the methanol extract of Ecbalium elaterium (16 ± 1.0 mm) and Mandragora autumnalis (18.7 ± 1.0 mm) at 100 mg/ml of each extract (Obeidat et al, 2012).…”

Section: Determination Of Mechanisms Of Antibiosis (Bactericidal or Bmentioning

confidence: 83%

GC-MS analysis and antimicrobial effects of methanol stem bark extract of Trilepisium madagascriense DC.

Olajuyigbe¹,

Faith²,

Adeoye-Isijola³

2017

ijSciences

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ABSTRACT:The therapeutic potentials of methanol stem bark extract of Trilepisium madagascriense was determined using Gas Chromatography-Mass Spectrometry to identify its bioactive compounds of pharmaceutical importance while the antimicrobial activities were assayed in vitro by agar well diffusion and macrobroth dilution techniques against different microbial isolates. The mass spectra of the identified compounds in the extract at different retention time showed the presence of ethyl iso-allocholate, (3β,5Z,7E)-9,10-Secocholesta-5,7,10(10)-triene-3,24,25-triol, 2,6-Dimethoxyamphetamine, 4-Hexenoic acid, 4-methyl-6-(fluorodimethylsilyl)-6-trimethysily-, 2-methoxy-4-(methoxymethyl)-Phenol, 2-methoxy-1,4-Benzenediol, 2,4-Dimethoxyphenol, Indole, Paromomycin, Hydroquinone and Tetrahydro-N-[(tetrahydro-2-furanyl)methyl-2-Furanmethanamine amongst other bioactive compounds of therapeutic potentials. This extract showed antimicrobial activities. At the lowest concentration of 25 mg/ml, 100 µl of the extract produced inhibition zones ranging between 14 and 18 ± 1.0 mm and inhibition zones ranging between 18 and 28 ± 1.0 mm in all the isolates at the highest concentration of 100 mg/ml. While the bacterial MICs ranged between 1.25 and 5 mg/ml and the MBCs ranged between 2.5 and 10 mg/ml, the fungal MICs ranged between 0.098 and 12.5 mg/ml while the MFCs ranged between 0.781 and <25 mg/ml. With exception of MIC index of Klebsiella pneumoniae ATCC 10031 which was equal to 4, the MIC index of other isolates ranged between 1 and 2. Klesbiella pneumoniae ATCC 10031 and Proteus vulgaris CSIR 0030 had the highest MICs of 1.25 mg/ml, followed by B. cereus ATCC 10702, Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 19582, Enterococcus faecalis ATCC 29212 and Bacillus subtilis KZN with MICs of 2.5 mg/ml while Escherichia coli ATCC 25922, Enterococcus cloacae ATCC 13047, Enterococcus faecalis KZN, Shigella sonnei ATCC 29930, Klebsiella pneumoniae KpFa, Staphylococcus aureus SaFa, Escherichia coli EcFa and Pseudomonas aeruginosa PmFa had the least MICs of 5.0 mg/ml. Enterococcus faecalis KZN, Bacillus subtilis KZN and Proteus vulgaris CSIR 0030 had the highest MBCs of 2.5 mg/ml. Although Candida albicans had MICs ranging between 0.098 mg/ml and Candida tropicalis had the least MICs of 12.5 mg/ml, the MFCs were 0.781 mg/ml and 25 mg/ml. This study shows that the pharmacological effects of Trilepisium madagascriense depends on bioactive compounds identified while this plant is a source for isolating novel drugs having significant therapeutic potentials.

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“…Natural herbal products offer new antimicrobial agents and a huge number of novel drug components have been isolated from plants (Nascimento et al, 2000;Obeidat et al, 2012). The wild collected and consumed plants by people have lesser side effects once compared to synthetic or semi-synthetic drug agents.…”

Section: Introductionmentioning

confidence: 99%

“…The wild collected and consumed plants by people have lesser side effects once compared to synthetic or semi-synthetic drug agents. A huge number of preliminary studies have been performed in order to determine the potent antimicrobial agents (Obeidat et al, 2012;Molla et al, 2010;Kang et al, 2011). physiological processes in cellular metabolism (Lozak et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Research on selected wild edible vegetables: Mineral content and antimicrobial potentials

Akgunlu¹,

Şekeroğlu²,

Çalışkan³

et al. 2016

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The present study was designed to examine the antimicrobial activity and to determine mineral contents of commonly consumed wild vegetables, which utilized mostly in southern parts of Turkey. Ten plantsbelonging to the different taxa (Arum dioscoridis Sm., Chenopodium album L., Malva sylvestris L., Mentha longifolia (L) Huds., Nasturtium officinale W.T. Aiton, Papaver rhoeas L., Polygonum aviculare L., Rumex acetosella L., Sinapis alba L. and Urtica dioica L.) were individually screened for their possible antibacterial and antifungal activities using both polar (methanolic) and nonpolar (n-hexane) crude extracts. Theextractswere tested against both standard American Type Culture Collection (ATCC) and Refik Saydam Turkish National Type Culture Collection (RSKK) and clinically isolated (CI) bacterial strains and two ATCC fungal strains.The efficacy of each plant extracts was tested by serial micro dilution method (MIC). Analyzed plant extracts demonstrated antimicrobial activity with the MIC values ranging 16 to 64 µg/ml against culture collection Gram negative bacteria and  256 µg/ml against isolated strains. The MIC values for selected extracts ranged from 16 to 256 µg/ml against culture collection Gram positive bacteria and  256 µg/ml against isolated strains. Among the plants tested, C. album (aqueousmetha nolic) extra cts demonstra ted the highest antimicrobial activities againsta ll the test microorganisms with the best MIC (16 µg/ml) value. Furthermore, major and trace element concentrations were also screened and evaluated for their potential risk for public health by comparing with established limits proposed by various scientific reports.

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Antimicrobial Activity of Crude Extracts of Some Plant Leaves

Cited by 113 publications

References 12 publications

Antioxidant, antibacterial and cytotoxic effects of the phytochemicals of whole Leucas aspera extract

Antioxidant, antibacterial and cytotoxic effects of the phytochemicals of whole Leucas aspera extract

GC-MS analysis and antimicrobial effects of methanol stem bark extract of Trilepisium madagascriense DC.

Research on selected wild edible vegetables: Mineral content and antimicrobial potentials

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