Antimicrobial Activity of Elephant Garlic Oil against<i>Vibrio cholerae</i><i>in Vitro</i>and in a Food Model

Rattanachaikunsopon, Pongsak; Phumkhachorn, Parichat

doi:10.1271/bbb.90128

Cited by 32 publications

(23 citation statements)

References 19 publications

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“…GarO inhibits planktonic growth of P. aeruginosa and S. aureus Previous studies have reported the inhibitory effect of garlic and garlic extract against planktonic cells of Grampositive and Gram-negative bacteria (Al-Waili et al, 2007;Rattanachaikunsopon & Phumkhachorn, 2009;Sarkar & Chakraborty, 2008;Tsao et al, 2003). Our goal was to develop a GarO that would inhibit the colonization of wounds and the development biofilms by different bacterial pathogens.…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies have demonstrated the effectiveness of garlic (Allium sativum) or garlic extract (especially allicin) in inhibiting the growth of different bacterial pathogens (Al-Waili et al, 2007;Rattanachaikunsopon & Phumkhachorn, 2009;Sarkar & Chakraborty, 2008;Tsao et al, 2003). Garlic extract has also been shown to increase the susceptibility of P. aeruginosa to tobramycin and to phagocytosis Rasmussen et al, 2005), and, in the murine model of lung infection, it has been shown to significantly reduce mortality rate and promote rapid clearing of P. aeruginosa from the lung .…”

Section: Introductionmentioning

confidence: 99%

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Garlic ointment inhibits biofilm formation by bacterial pathogens from burn wounds

Nidadavolu

Amor

Tran

et al. 2012

Journal of Medical Microbiology

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Garlic ointment inhibits biofilm formation by bacterial pathogens from burn wounds

Nidadavolu

Amor

Tran

et al. 2012

Journal of Medical Microbiology

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“…Garlic has been used as a medicine since ancient times and has long been known to have antibacterial, antiviral, antifungal, antialgal, antiproteolytic, anticancer, antithrombotic, antiatherosclerotic, antioxidant, and immune modulation properties (4,5,12,16,45,58,59,60,66,69,70,77). Recently, garlic extract was shown to have effective broad-spectrum antimicrobial activity (11,15,21,44,62,74), including activity against oral bacteria (8). The main antimicrobial constituent of garlic has been identified as the oxygenated sulfur compound allicin.…”

mentioning

confidence: 99%

“…These compounds possess potent anticancer, antioxidant, and anti-inflammatory immune modulation activities and antimicrobial properties (2,30,71,72,79,83). The inhibitory effect of DASs against Bacillus cereus, Salmonella sp., and Vibrio cholerae (7,62,63), Staphylococcus aureus and Candida albicans (42,51), Helicobacter pylori (52,58), and Klebsiella pneu-moniae and Pseudomonas aeruginosa (17,37,53,73) were studied, and a recent report has shown that DASs were synergistically effective when combined with antibiotics against antibiotic-resistant strains of Escherichia coli, Enterobacter cloacae, E. faecalis, and Citrobacter freundii (80). On the basis of the results of previous studies, we hypothesize that DAS is effective against A. actinomycetemcomitans and that it retains its activity even after heat treatment.…”

mentioning

confidence: 99%

In Vitro Efficacy of Diallyl Sulfides against the Periodontopathogen Aggregatibacter actinomycetemcomitans

Velliyagounder

Ganeshnarayan

Velusamy

et al. 2012

Antimicrob Agents Chemother

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The in vitro antibacterial effects of diallyl sulfide (DAS) against the Gram-negative periodontopathogen Aggregatibacter actinomycetemcomitans, the key etiologic agent of the severe form of localized aggressive periodontitis and other nonoral infections, were studied. A. actinomycetemcomitans was treated with garlic extract, allicin, or DAS, and the anti-A. actinomycetemcomitans effects of the treatment were evaluated. Garlic extract, allicin, and DAS significantly inhibited the growth of A. actinomycetemcomitans (greater than 3 log; P < 0.01) compared to control cells. Heat inactivation of the garlic extracts significantly reduced the protein concentration; however, the antimicrobial effect was retained. Purified proteins from garlic extract did not exhibit antimicrobial activity. Allicin lost all its antimicrobial effect when it was subjected to heat treatment, whereas DAS demonstrated an antimicrobial effect similar to that of the garlic extract, suggesting that the antimicrobial activity of garlic extract is mainly due to DAS. An A. actinomycetemcomitans biofilm-killing assay performed with DAS showed a significant reduction in biofilm cell numbers, as evidenced by both confocal microscopy and culture. Scanning electron microscopy (SEM) analysis of DAS-treated A. actinomycetemcomitans biofilms showed alterations of colony architecture indicating severe stress. Flow cytometry analysis of OBA9 cells did not demonstrate apoptosis or cell cycle arrest at therapeutic concentrations of DAS (0.01 and 0.1 g/ml). DAStreated A. actinomycetemcomitans cells demonstrated complete inhibition of glutathione (GSH) S-transferase (GST) activity. However, OBA9 cells, when exposed to DAS at similar concentrations, showed no significant differences in GST activity, suggesting that DAS-induced GST inhibition might be involved in A. actinomycetemcomitans cell death. These findings demonstrate that DAS exhibits significant antibacterial activity against A. actinomycetemcomitans and that this property might be utilized for exploring its therapeutic potential in treatment of A. actinomycetemcomitans-associated oral and nonoral infections.

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“…Antibacterial properties of garlic extract or allicin have been reported against a number of pathogenic bacteria viz. E. coli 17 , V. cholerae 18,19 , Pseudomonas aeruginoa 20 , Salmonella typhimurium 18 , Streptococcus pneumoniae 21 , Staphylococcus aureus 22 and Methicillin resistant S. aureus (MRSA) 23,24 . Present study was aimed to check the efficacy of garlic extract against multidrug resistant V. cholerae having epidemic potential isolated from different outbreaks in India.…”

Section: Introductionmentioning

confidence: 99%

Bactericidal Efficacy of Allium sativum (garlic) Against Multidrug Resistant Vibrio cholerae O1 Epidemic Strains

Kumar¹,

Yadav²,

Jain³

et al. 2016

Def. Sc. Jl.

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In recent years, emerging trend of antibiotic resistance in Vibrio cholerae associated with cholera epidemics is a matter of serious concern for the management of the disease. Indiscriminate use of antibiotics generally results in selection of antibiotic resistant strains. Introduction of newer antibiotics is a challenging task for the researchers as bacteria soon attain resistance. Therefore, identifying natural compounds of medicinal importance for control of cholera would be the best alternative. Garlic (Allium sativum) was recognised for many centuries in early Chinese, Egyptian and Indian civilisations as an herbal or traditional medicine. In present study, garlic was selected for screening of antimicrobial efficacy against V. cholerae. A total of 55 V. cholerae strains isolated from various outbreaks/epidemics were subjected to antimicrobial testing as per CLSI, USA 2010 guidelines. Antimicrobial screening of garlic extract was performed against all the multidrug resistant strains of V. cholerae. The garlic extracts showed antibacterial activity against all the V. cholerae strains tested, irrespective of their origin, multidrug resistance and virulence. Antibacterial efficacy of garlic on V. cholerae was also evident from in vivo study on sealed adult mice model. Thus, the Garlic extract harnesses the potential to control infection of multidrug resistant V. cholerae, especially in outbreak like situations in remote and under developed areas where drug supply itself is a challenge.

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Antimicrobial Activity of Elephant Garlic Oil againstVibrio choleraein Vitroand in a Food Model

Cited by 32 publications

References 19 publications

Garlic ointment inhibits biofilm formation by bacterial pathogens from burn wounds

Garlic ointment inhibits biofilm formation by bacterial pathogens from burn wounds

In Vitro Efficacy of Diallyl Sulfides against the Periodontopathogen Aggregatibacter actinomycetemcomitans

Bactericidal Efficacy of Allium sativum (garlic) Against Multidrug Resistant Vibrio cholerae O1 Epidemic Strains

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