In this study, the antimicrobial activity of some medicinal plant seeds from the Apiaceae family which are anise, cumin, and fennel was investigated. Moreover, ethanol (70%) and methanol (80%) extracts of anise, cumin, and fennel seeds and essential oils of them were performed for this aim. Escherichia coli ATCC 25922 and Salmonella Enteritidis ATCC 13076, Bacillus subtilis ATCC 6633, Streptecoccus mutans ATCC 25175 and Listeria monocytogenes ATCC7644 were utilized as test microorganisms. Then, essential oils and ethanol extracts of cumin seeds showed the highest antimicrobial effects on almost all of the test microorganisms. While methanol extracts of fennel seeds had the highest antimicrobial effects against St. mutans and B. subtilis when compared to other methanol extracts. Additionally, methanol extracts of anise seeds indicated the highest effect on E. coli. However, all types of medicinal plant seeds had antimicrobial effects on test microorganisms in various severities (7.00-20.00 mm zone diameter) except L. monocytogenes. Thus, the results support existing scientific evidence for the evaluation of these plants as natural antimicrobial agents in several industries including food and drugs.