The purpose of this study was to compare to evaluate the post operative pain with different calcium hydroxide formulations when used as intracanal medicament in root canal which was evaluated by VAS scale. The 150 subjects were randomly assigned to four groups and one control group depending upon the intracanal medicament: Group 1: Calcium hydroxide paste (Ivoclar) was used as intracanal medicament, Group 2: Calcium hydroxide paste with iodoform (Metapex) was used as intracanal medicament, Group 3: Calcium hydroxide points (Hygenic) was used as intracanal medicament, Group 4: Calcium hydroxide paste using chitosan (0.2%) as vehicle was used as intracanal medicament, Group 5: Control group containing dry sterile cotton. The incidence and intensity of preoperative pain and experienced post operative pain (on 6, 12, 24 hour and 48 hour) after first visit of treatment were assessed according to patient’s recordings in visual analogue scale. The medicament causing least operative pain are ranked according to superiority as Calcium hydroxide paste > Calcium hydroxide points > Calcium hydroxide with chitosan > calcium hydroxide with iodoformAt 6 postoperative hour, least post operative pain was recorded by the calcium hydroxide points and high post operative pain in calcium hydroxide with iodine (Metapex). At 12th, 24th, 48 postoperative hour least post operative pain was recorded by calcium hydroxide paste (Apexcal).