Antimicrobial Activity of Leaf of Sphaeranthus Indicus against Some Selected Human Pathogenic Bacteria

Venkatachalam, Dhanapal

doi:10.22270/jmpas.v7i2.754

Cited by 5 publications

(4 citation statements)

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“…13 Similar antibacterial activity was observed in other plants of the same family. 14 Higher inhibition zone was observed in B. subtilis at 5 mg/disc for hexane flower extract. 15 The inhibition zone was directly proportional to the concentration used.…”

Section: Discussionmentioning

confidence: 94%

Sphaeranthus indicus: A Promising Antimicrobial Agent

Saxena¹,

Upadhyay²,

Lodha

2021

FRA

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The aim of the present study was to determine the antimicrobial property of the solvent extracts of flowers and aerial parts of S. indicus. Methods: The flowers and the aerial parts of Sphaeranthus indicus were extracted with n-hexane, benzene, chloroform, ethyl acetate and acetone respectively. The extracts were analyzed for the antimicrobial effect by disc diffusion method at concentrations of 5, 2.5 and 1.25 mg/disc. The Minimum Inhibitory Concentration (MIC) was tested using broth micro dilution method at concentrations ranging from 5 to 0.039 mg/ml. Results: There was a significant antibacterial and antifungal activity in hexane extract of flower and aerial parts. The MIC was seen at 0.15 mg/ml against Staphylococcus aureus and the highest MIC (5 mg/ml) was noted for S. epidermidis. The n-hexane extracts of flower and aerial parts showed MIC as 0.15 and 1.25 mg/ml respectively against Candida albicans. Conclusion: Concluding it can be said that the S. indicus flower n-hexane extract showed promising antimicrobial agent.

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Section: Discussionmentioning

confidence: 94%

Sphaeranthus indicus: A Promising Antimicrobial Agent

Saxena¹,

Upadhyay²,

Lodha

2021

FRA

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“…7,8 Furthermore, the current price of most of the chemotherapeutic agents is on the rise hence steps should be taken to develop effective natural, non-toxic drug for treatment. 9 Hence the present study was performed to explore the antimicrobial property of S.indicus. The whole plant ethanolic extract of S. indicus failed to demonstrate any antibacterial property against E. coli, Pseudomonas aeruginosa, Proteus mirabilis and Staphylococcus aureus in 3 doses (100 mcg, 200 mcg and 300 mcg when compared with ciprofloxacin (30 mcg) as standard drug.…”

Section: Discussionmentioning

confidence: 99%

In vitro evaluation of antibacterial activity in ethanolic extract of whole plant Sphaeranthus indicus Linn.

Sundaresan¹,

Kesavan

2020

Int J Basic Clin Pharmacol

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Background: Sphaeranthus indicus Linn is a widely used medicinal plant in Indian traditional system of medicine against human pathogens. Alarming bacterial resistance is urging scientist to search for newer anti-microbial substances from the medicinal plants. The objective of the study was to evaluate the antibacterial activity of ethanolic extract of the whole plant Sphaeranthus indicus Linn (Asteraceae).Methods: The antibacterial activity of ethanolic extract of whole plant of Sphaeranthus indicus Linn was done against Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis and Staphylococcus aureus in Mueller Hinton Agar (MHA) and compared with ciprofloxacin as standard by disc diffusion method.Results: The study revealed that there was no zone of inhibition in doses of 100 mcg, 200 mcg and 300 mcg of ethanolic whole plant extract of Sphaeranthus indicus in MHA plates compared with ciprofloxacin 30 mcg.Conclusions: Ethanolic extract of Sphaeranthus indicus does not have antibacterial activity. Further studies are needed in different extracts and parts of the plant. Simultaneous studies can be done in different places to evaluate environmental factors and regional variations.

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“…Te antifungal activity of the various extracts was determined by comparing them with the antifungal agents fuconazole (25 μg disc − 1 ) (Oxoid, UK) and DMSO. Te results of antimicrobial activity were evaluated using a Himedia zone reader [34][35][36].…”

Section: Antifungal Activity Assaymentioning

confidence: 99%

Screening of Preliminary Phytochemicals, Molecular Identification, and Antimicrobial and Anti‐Inflammatory Activity of Justicia gendarussa

Ali

Mahmud

Mohiuddin

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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Justicia gendarussa is a well-known medicinal plant from ancient times that has been using to treat a variety of ailments. The main objectives of the current study were to assess the phytochemicals, molecular identification of the plant, and determination of MIC, as well as antimicrobial and anti-inflammatory activities of various types of plant extracts (aqueous, ethanolic, methanolic, diethyl-ether, dichloromethane, and ethyl-acetate extracts) of J. gendarussa (leaves) against 11 human pathogens, including bacteria and fungi. In the present study, J. gendarussa was identified as a medicinal plant based on ITS gene amplification by PCR. The screening of the presence of important phytochemicals that carry high significance from a therapeutic standpoint was also carried out. The antimicrobial activity was assessed by following the disc diffusion and broth dilution procedures. The highest antimicrobial activity was observed against S. aureus (16 mm), S. flexneri (15 mm), and C. albicans (15 mm) compared with gentamicin (10 μg/disc) and fluconazole (25 μg/disc). Besides this, methanolic extract showed the highest anti-inflammatory activity (76% membrane protection). The MIC values (8–2048 μg/ml) of aqueous extract were observed against both types of bacteria and fungi, with the highest MIC value recorded at (2048 μg/ml) for S. flexneri. This study is the primary investigation in Bangladesh where molecular identification of this medicinal plant was carried out and the anti-inflammatory activity of this plant was evaluated by the HRBC membrane stabilization method. In conclusion, our findings suggest that this plant may be useful in research and development for discovering a broad spectrum of modern herbal medicines.

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Antimicrobial Activity of Leaf of Sphaeranthus Indicus against Some Selected Human Pathogenic Bacteria

Cited by 5 publications

References 0 publications

Sphaeranthus indicus: A Promising Antimicrobial Agent

Sphaeranthus indicus: A Promising Antimicrobial Agent

In vitro evaluation of antibacterial activity in ethanolic extract of whole plant Sphaeranthus indicus Linn.

Screening of Preliminary Phytochemicals, Molecular Identification, and Antimicrobial and Anti‐Inflammatory Activity of Justicia gendarussa

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