Antimicrobial Activity of MTA Fillapex, Real Seal SE, Acroseal and Zinc oxide Eugenol Sealers against Enterococcus Faecalis and Candida Albicans

Rahman, Hena; Chandra, Ramesh; Chowdhary, Divya; Singh, Shailja; Tripathi, Supratim; Anwar, Syed Zaki

doi:10.9790/0853-1601046669

Cited by 5 publications

(7 citation statements)

References 22 publications

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“…Numerous studies have been performed to assess the antimicrobial efficacy using ADT. 1 , 12 – 16 It is one of the frequent and commonly used methods in vitro for assessing the antimicrobial efficacy. According to Ramachandra et al, ADT maintains the chemical properties of the tested extracts throughout the experimental period.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Antimicrobial Efficacy of Four Different Plant Extracts against Cariogenic Bacteria: An In Vitro Study

Rai

Tripathi

Saha

et al. 2020

International Journal of Clinical Pediatric Dentistry

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A bstract Background Dental caries is an oral disease which is infectious in nature associated with various pathogenic microorganisms and is prevalent across the world. Aim To evaluate and compare the anticariogenic properties of the different plant extracts against various cariogenic microorganisms. Materials and methods In phase I, the anticariogenic efficacy of four different herbal extracts, namely Ocimum sanctum ( Tulsi ), Terminalia chebula ( Harad ), Tinospora cordifolia ( Guduchi ), and Glycyrrhiza glabra (Licorice), was evaluated against two strains of bacteria viz. Streptococcus mutans and Lactobacillus acidophilus. In the second phase of the study, the overall decay depth was compared. Results In phase I, zone of inhibition was significantly higher in G. glabra (Licorice) followed the descending order by O. sanctum ( Tulsi ), T. c hebula ( Harad ), and T. cordifolia ( Guduchi ), respectively. In the second phase, it was found that the decay depth was significantly higher in T. cordifolia ( Guduchi ) followed the descending order by T. chebula ( Harad ), O. sanctum ( Tulsi ), and G. glabra (Licorice), respectively. Conclusion Glycyrrhiza glabra (Licorice) extract had potent antibacterial efficacy against S. mutans and L. acidophilus. Clinical significance Synthetic drugs have many adverse effects, so more attention has been paid to natural remedies because they are safe and effective. Today, the main interest is shifted toward the drugs which is derived from plants, which has led to the screening of many herbal plants for their potential antimicrobial activity and the same can be used clinically as an alternative of synthetic drugs used in dentistry. How to cite this article Rai A, Tripathi AM, Saha S, et al . Comparison of Antimicrobial Efficacy of Four Different Plant Extracts against Cariogenic Bacteria: An In Vitro Study. Int J Clin Pediatr Dent 2020;13(4):361–367.

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Section: Discussionmentioning

confidence: 99%

Comparison of Antimicrobial Efficacy of Four Different Plant Extracts against Cariogenic Bacteria: An In Vitro Study

Rai

Tripathi

Saha

et al. 2020

International Journal of Clinical Pediatric Dentistry

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“…MTA has less aluminum and more iron (Fe). A decrease in setting time can be achieved by adding iron (Fe) and calcium chloride (CaCl) [14,19,36]. Zirconium (Zr) is found only in the Tricalcium silicate-WPC added Bi 2 O 3 and functions as a radioopacifier [32 -36].…”

Section: Discussionmentioning

confidence: 99%

Antifungal Effectiveness between Tricalcium Silicate-White Portland Cements Added Bi2O3 and Mineral Trioxide Aggregate Against Candida albicans

Nurdin¹,

Sari²,

Adang³

et al. 2020

TODENTJ

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Background Candida albicans is the most dominant fungus found in root canal reinfection cases. This microorganism can withstand extreme pH, low oxygen levels, lack of nutrients, and penetrate the dentinal tubules so that it can resist the intracanal medicament. Root canal cement helps prevent microorganisms and causing root canal reinfection. MTA is one of the root canals cement which is widely used and has an excellent antifungal activity, but it is less beneficial economically. Tricalcium silicate-white Portland cement (WPC) has a similar main composition as MTA, except there is no Bi2O3 content that functions as a radiopacifier. Objectives: To fabricate a mixture of tricalcium silicate-WPC with Bi2O3 through a simple solution method and investigate antifungal activity's effectiveness between tricalcium silicate-WPC added Bi2O3 and MTA to C. albicans. Materials and Methods: The 80 g of tricalcium silicate-WPC was mixed with 20 g of Bi2O3 through the simple solution method using 99.9% isopropanol as a solution. This sample solution is stirred until homogeneous, then centrifuged. The precipitate was dried until a dry powder was obtained. The powder was analyzed using X-Ray fluorescence spectrometry to identify its chemical composition and concentration. A total of 30 samples were divided into two experimental groups of tricalcium silicate-WPC added Bi2O3 and ProRoot MTA. The vials, which contain cement and C. albicans, respectively, were incubated at 37°C for 24 hours and diluted to obtain a suspension 104, 106 (0.5 in McFarland’s nephelometer) and then inoculated with sterile cotton swabs onto Saboroud Dextrose Agar Plates. The plates were incubated at 37°C for 24 hours. The measurement of colony number of C. albicans was counted by colony counter (CFU/ml). Results: The Bi2O3 was revealed in tricalcium silicate-WPC based on XRF characterization, and the antifungal test showed that both materials were effective against C. albicans. There was no statistically significant difference in the number of C. albicans colonies between tricalcium-WPC added Bi2O3 and MTA (p>0.05). Conclusion: The mixture of Bi2O3 in tricalcium silicate-WPC was successfully fabricated through a simple solution method, and both samples were effective against the C. albicans.

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“…The methodology used in this study was adapted from Damasceno et al 17 (2008), Gomes et al 18 (2004), and Rahman et al 19 (2017). An agar diffusion test was used to determine inhibitory effects against three fungal species: Candida albicans (ATCC 10231), Candida glabrata (ATCC 90030), and Candida tropicalis (ATCC 750).…”

Section: Microbiological Analysismentioning

confidence: 99%

“…The plates were kept for 2 hours at room temperature to allow the diffusion of the sealers through the agar, and then they were transferred to a 37 °C aerobic incubation environment for 24 hours to allow microbial growth (Tanomaru-Filho et al 21 (2007), Gomes et al 18 (2004)). Growth inhibition zones were measured daily for 7 days using a manual caliper (Damasceno et al 17 (2008), Rahman et al 19 (2017)).…”

Section: Microbiological Analysismentioning

confidence: 99%

Comparative in vitro analysis of the antifungal activity of different calcium silicate-based endodontic sealers

Moreira,

Peña-Bengoa,

Niklander

et al. 2024

Braz. J. Oral Sci.

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Aim: This study aimed to perform an in vitro comparative analysis of the antifungal activity of different calcium silicate-based endodontic sealers against three fungal species. Methods: The antifungal properties of three calcium silicate-based sealers were tested: Bio-C Sealer, Cambiar a Sealer Plus BC, and MTA-Fillapex. Two commonly used sealers were used as controls: AH Plus and Endomethasone. An agar diffusion test was performed to analyze the antifungal activity of the sealers against Candida albicans, Candida glabrata, Candida tropicalis, and a mixed microbial culture medium. The results were analyzed using ANOVA (p <0.05). Results: Endomethasone exhibited the highest inhibition against all strains examined, maintaining a consistent level of inhibition throughout 7 days. MTA-Fillapex demonstrated the best performance among the calcium silicate-based sealers for the three fungal species (p < 0.05), maintaining stable values over the 7 days, surpassing that of Endomethasone. Nevertheless, MTA-Fillapex only exhibited antimicrobial effect against the mixed culture for the first 24 hours, and no antimicrobial activity was observed at 48 hours, being surpassed by all tested sealers (p < 0.05). Conclusion: Of all silicate-based sealers tested, only MTA-Fillapex exhibited promising antifungal activity. Nevertheless, care must be taken when extrapolating these results, as MTA-Fillapex exhibited poor antimicrobial activity when tested in mixed microbial cultures.

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Antimicrobial Activity of MTA Fillapex, Real Seal SE, Acroseal and Zinc oxide Eugenol Sealers against Enterococcus Faecalis and Candida Albicans

Abstract: Abstract:Aim:The aim of the present study was to evaluate the antimicrobial activity of different root canal sealers (MTA Fillapex, Real Seal SE, Acroseal and Zinc Oxide Eugenol)

Cited by 5 publications

References 22 publications

Comparison of Antimicrobial Efficacy of Four Different Plant Extracts against Cariogenic Bacteria: An In Vitro Study

Comparison of Antimicrobial Efficacy of Four Different Plant Extracts against Cariogenic Bacteria: An In Vitro Study

Antifungal Effectiveness between Tricalcium Silicate-White Portland Cements Added Bi2O3 and Mineral Trioxide Aggregate Against Candida albicans

Comparative in vitro analysis of the antifungal activity of different calcium silicate-based endodontic sealers

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