Antimicrobial Activity of N-Hexane Extract of Nigella Sativa against Some Pathogenic Bacteria

Abraham, Ayanwale O

doi:10.34297/ajbsr.2019.06.001077

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…The study of Khalid et al [ 36 ] demonstrated that the aqueous extract has a weak antibacterial activity toward S. aureus , E. coli , P. aeruginosa , and E. faecalis , while in the present study it was demonstrated that all tested strains manifested a high sensitivity to the NS aqueous fraction. Concerning the n-hexane fraction, the MIC values ranged from 5 to 20 mg/mL, which was close to those obtained in the Abraham et al study [ 37 ].…”

Section: Resultssupporting

confidence: 88%

Loaded n-Hydroxyapatite/SSG 3D Scaffolds as a Drug Delivery System of Nigella sativa Fractions for the Management of Local Antibacterial Infections

et al. 2022

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As a result of their close similarities to the inorganic mineral components of human bone, hydroxyapatite nanoparticles (n-HAp) are widely used in biomedical applications and for the elaboration of biocompatible scaffold drug delivery systems for bone tissue engineering. In this context, a new efficient and economic procedure was used for the consolidation of n-HAp in the presence of various Nigella sativa (NS) fractions at a near-room temperature. The research conducted in the present study focuses on the physicochemical properties of loaded n-HAp 3D scaffolds by NS fractions and the in vitro antibacterial activity against Gram-negative (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 27853), and Gram-positive (Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 700603) bacteria. In order to better understand the effect of the inserted fractions on the HAp molecular structure, the elaborated samples were subject to Fourier transform infrared (FTIR) and X-ray diffraction (XRD) spectroscopic analyses. In addition, the morphological investigation by scanning electron microscope (SEM) of the loaded n-HAp 3D scaffolds demonstrated the presence of a porous structure, which is generally required in stimulating bone regeneration. Furthermore, the fabricated 3D composites exhibited significant antibacterial activity against all tested bacteria. Indeed, MIC values ranging from 5 mg/mL to 20 mg/mL were found for the HAp-Ethanol fraction (HAp-Et) and HAp-Hexane fraction (HAp-Hex), while the HAp-Aqueous fraction (HAp-Aq) and HAp-Methanol fraction (HAp-Me) showed values between 20 mg/mL and 30 mg/mL on the different strains. These results suggest that the HAp-NS scaffolds were effective as a drug delivery system and have very promising applications in bone tissue engineering.

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Section: Resultssupporting

confidence: 88%

Loaded n-Hydroxyapatite/SSG 3D Scaffolds as a Drug Delivery System of Nigella sativa Fractions for the Management of Local Antibacterial Infections

et al. 2022

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“…Khalid et al (2011) investigated three extraction method namely cold water, hot water and methanol for N. sativa seeds and amongst them cold water extracts were successful against S. aureus. In another study using n-hexane for the extraction of black cumin, potentially inhibitory effect against S. aureus was reported by researchers (Abraham et al, 2019). The existence of these potential activities has led researchers to find their active ingredients.…”

Section: Nigella Sativa Lmentioning

confidence: 99%

A review on antimicrobial activities of some culinary herbs and spices against Staphylococcus aureus

Berber

TOKSÖZ

Türkmenoğlu

et al. 2022

Frontiers in Life Sciences and Related Technologies

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Food safety is of great importance all over the world as it concerns consumer health. All employees in the food chain must comply with the hygiene rules. One of the important issues that threaten food safety is contamination with microorganisms. Numerous people are affected by contaminated and/or poorly preserved food and outbreaks have occurred. The World Health Organization (WHO) draws attention to human health and economic losses in this respect. From ancient times, herbs and spices are utilized in Turkey and various parts of the world and improved the flavor of food and their sensory properties. It is also possible to prevent the development of Staphylococcus aureus, which causes food poisoning, thanks to the antibacterial properties of culinary herbs or spices. Natural antimicrobial substances from spices and herbs may be alternative strategies in inhibition/elimination of growth of and in food preservation of S. aureus extending the shelf life without synthetic preservatives. This review aims to explain foodborne diseases and their global burden, staphylococcal food poisoning, natural antimicrobials, some edible herbs in Turkey: their culinary uses and antibacterial efficacy against S. aureus.

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“…Black seeds are rich in crude fiber, minerals like Ca2+, Fe 3+, and K+, fatty acids such as oleic, linoleic, and palmitic acids, terpenoids, and alkaloids such as nigellidine, nigellimine, and nigellicine (Hassan et al, 2017). Nigella sativa seeds contains the important active compounds called thymoquinone, dithymoquinone, thymohydroquinone, thymol, carvacrol, nigellimine-N-oxide, saponins and alpha-hederin (Abraham, 2019;Aljabre et al, 2015), which have a significant function in the treatment of diabetes .…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition and In vitro Antibacterial and Cytotoxic Effect of Nigella sativa L. Seed Extract

Callixte

Arwati

Irene

et al. 2021

Kemas

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Nigella sativa L. is a plant with high medicinal profile in the treatment of diseases. This study aimed to assess the chemical composition and in vitro antibacterial and cytotoxic effect of Nigella sativa L. seed extract. Seeds were purchased from supermarket and blended into powder using electric blender. The powder was macerated with 96% methanol. The extracts were then qualitatively screened to test the presence of secondary metabolites and agar well diffusion method was employed to study the antibacterial activity of extracts. In this evaluation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to examine the cytotoxic effect of extract on peripheral blood mononuclear cells (PBMCs). MTT assay was done on different extract concentrations for 3 days and the results were recorded daily. The findings revealed the presence of tannins, terpenoids, steroids, saponins, alkaloids, phenolic compounds and flavonoids. The antibacterial activity was observed with the inhibition zones ranging from 11.3 ±1.1 to 16.3 ±1.5mm while the minimum inhibitory concentration (MIC) values of extract ranged between 0.5 and 1.25 μg/mL. The black seed extracts contain bioactive substances with germicidal activity and in vitro administration of black seed extract up to the dose of 100 μg/mL resulted no cytotoxic effect on PBMCs.

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Antimicrobial Activity of N-Hexane Extract of Nigella Sativa against Some Pathogenic Bacteria

Abstract: This work is licensed under Creative Commons Attribution 4.0 License AJBSR.MS.ID.001077.

Cited by 4 publications

References 19 publications

Loaded n-Hydroxyapatite/SSG 3D Scaffolds as a Drug Delivery System of Nigella sativa Fractions for the Management of Local Antibacterial Infections

Loaded n-Hydroxyapatite/SSG 3D Scaffolds as a Drug Delivery System of Nigella sativa Fractions for the Management of Local Antibacterial Infections

A review on antimicrobial activities of some culinary herbs and spices against Staphylococcus aureus

Chemical Composition and In vitro Antibacterial and Cytotoxic Effect of Nigella sativa L. Seed Extract

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