Antimicrobial Activity of Penicillin G and N-acetylcystein on Planktonic and Sessile Cells of <i>Streptococcus suis</i>

Espinosa, Ivette; Báez, M; Lobo, Evelyn; Martínez, Siomara; Gottschalk, Marcelo

doi:10.5604/17331331.1197282

Cited by 5 publications

(2 citation statements)

References 15 publications

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“…It is one of the most important pathogens, and it is responsible for pneumonia, arthritis, septicemia, and meningitis in pigs and humans ( Bojarska et al, 2016 ). So far, some researchers have reported that S. suis is able to form biofilms ( Wang et al, 2015 ; Bojarska et al, 2016 ; Espinosa et al, 2016 ). Biofilm structures are packaged along with proteins, nucleic acids, extracellular polysaccharides (EPS), and other materials.…”

Section: Introductionmentioning

confidence: 99%

Sub-MICs of Azithromycin Decrease Biofilm Formation of Streptococcus suis and Increase Capsular Polysaccharide Content of S. suis

et al. 2016

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Streptococcus suis (S. suis) caused serious disease symptoms in humans and pigs. S. suis is able to form thick biofilms and this increases the difficulty of treatment. After growth with 1/2 minimal inhibitory concentration (MIC) of azithromycin, 1/4 MIC of azithromycin, or 1/8 MIC of azithromycin, biofilm formation of S. suis dose-dependently decreased in the present study. Furthermore, scanning electron microscopy analysis revealed the obvious effect of azithromycin against biofilm formation of S. suis. Especially, at two different conditions (1/2 MIC of azithromycin non-treated cells and treated cells), we carried out comparative proteomic analyses of cells by using iTRAQ technology. Finally, the results revealed the existence of 19 proteins of varying amounts. Interestingly, several cell surface proteins (such as ATP-binding cassette superfamily ATP-binding cassette transporter (G7SD52), CpsR (K0FG35), Cps1/2H (G8DTL7), CPS16F (E9NQ13), putative uncharacterized protein (G7SER0), NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (G5L259), putative uncharacterized protein (G7S2D6), amino acid permease (B0M0G6), and NsuB (G5L351)) were found to be implicated in biofilm formation. More importantly, we also found that azithromycin affected expression of the genes cps1/2H, cpsR and cps16F. Especially, after growth with 1/2 MIC of azithromycin and 1/4 MIC of azithromycin, the capsular polysaccharide content of S. suis was significantly higher.

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Section: Introductionmentioning

confidence: 99%

Sub-MICs of Azithromycin Decrease Biofilm Formation of Streptococcus suis and Increase Capsular Polysaccharide Content of S. suis

et al. 2016

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“…DNA extraction of isolated pathogen was done by using direct colony PCR (Espinosa et al, 2013). The colony from 24 to 48 hrs old culture was slightly touched by a sterilized tooth pick and added in an Eppendorf tube containing TE buffer (1.21g Tris 10mM & 0.29g EDTA added in 1L distilled water).…”

Section: Pcr Optimization Of Pathogenic Isolatesmentioning

confidence: 99%

Characterization of Erwinia amylovora causing Twig Blight of Loquat Tree in Loquat Growing Areas of Punjab and KPK, Pakistan

Mumtaz,

Naz,

Irshad

et al. 2024

Int. J. Phytopathol.

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Twig blight of loquat (Eriobotrya japonica Lindl.), caused by the gram-negative enterobacterium Erwinia amylovora, is a major constraint to loquat production worldwide. Recent surveys in loquat-growing areas of Punjab and KPK, Pakistan—specifically in Chakwal (Kalar Kahar, Choa Saidan Shah), Rawalpindi (Taxila, Wah), Murree (Tret and Chattar), and Haripur (Haripur, Khanpur, and Sara i Saleh)—revealed that this disease is highly prevalent. Twig blight compromises the health and vigor of loquat trees, often leading to their death, which discourages farmers from cultivating loquat commercially. Consequently, the area under loquat cultivation and its production have both been declining. Despite the severity of the issue, no systematic studies had been conducted to address this problem until now. A survey was carried out over two consecutive years (2016 and 2017) in 10 loquat-growing locations in Punjab and KPK (three orchards per location). The incidence of twig blight was higher in 2017 compared to 2016. The highest incidence was recorded in Taxila, with 69.74% in 2016 and 78.39% in 2017, while the lowest was in Kalar Kahar (20.86%). The incidence in Kalar Kahar showed a slight increase of less than 1% from the previous year, likely due to effective control measures implemented to limit the spread of the disease. The pathogen E. amylovora was isolated from diseased samples. Out of 102 bacterial isolates, 92 strains were gram-negative, and 72 tested positive in various biochemical assays (Levan, lipase, KOVAC oxidase, glucose fermentation, catalase oxidase, growth at 39°C, production of fluorescent pigment, and pectolytic activity tests). Three highly virulent isolates were identified through pathogenicity tests and confirmed by biochemical and molecular indicators. Molecular tagging of two chromosomal genes (amsB [MN902191] and Pst-glms [MN915084]) and one plasmid gene (pAE29 [MN902192]) was performed, confirming the identity of the isolates as E. amylovora. This study represents the first report of E. amylovora infecting loquat in Pakistan.

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Streptococcus suis biofilm: regulation, drug-resistance mechanisms, and disinfection strategies

Wang

Sun

et al. 2018

Appl Microbiol Biotechnol

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Streptococcus suis (S. suis) is a major swine pathogen and an important zoonotic agent. Like most pathogens, the ability of S. suis to form biofilms plays a significant role in its virulence and drug resistance. A better understanding of the mechanisms involved in biofilm formation by S. suis as well as of the methods to efficiently remove and kill biofilm-embedded bacteria can be of high interest for the prevention and treatment of S. suis infections. The aim of this literature review is to update our current knowledge of S. suis biofilm formation, regulatory mechanisms, drug-resistance mechanisms, and disinfection strategies.

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Antimicrobial Activity of Penicillin G and N-acetylcystein on Planktonic and Sessile Cells of Streptococcus suis

Cited by 5 publications

References 15 publications

Sub-MICs of Azithromycin Decrease Biofilm Formation of Streptococcus suis and Increase Capsular Polysaccharide Content of S. suis

Sub-MICs of Azithromycin Decrease Biofilm Formation of Streptococcus suis and Increase Capsular Polysaccharide Content of S. suis

Characterization of Erwinia amylovora causing Twig Blight of Loquat Tree in Loquat Growing Areas of Punjab and KPK, Pakistan

Streptococcus suis biofilm: regulation, drug-resistance mechanisms, and disinfection strategies

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