Antimicrobial Activity of Prodigiosin from Serratia sp. PDGS120915 Against Intestinal Pathogenic Bacteria

Ji, Keunho; Kim, Young Tae

doi:10.4014/mbl.1901.01006

Cited by 7 publications

(8 citation statements)

References 10 publications

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“…On the other hand, prodigiosin antimicrobial activity on Gram-negative bacteria appears to be strain-dependent. Gulani et al [9] reported that E. coli is resistant to prodigiosin as is E. coli KCTC 1116 [57]. However, we noted that our E. coli laboratory strain, E. coli OP50, is susceptible to prodigiosin, which is consistent with E. coli MG1655 susceptibility towards prodigiosin [26].…”

Section: Prodigiosin Selectively Antagonizes Gram-positive Bacteriasupporting

confidence: 85%

“…Our results demonstrated that the microbial growth and virulence factors of clinical pathogens are generally more resistant to prodigiosin compared to laboratory strains. Our results also mirror the resistance of clinical strains such as E. coli, P. aeruginosa [9], Salmonella Typhimurium KCTC 1926 [54], E. coli KCTC1116, Salmonella Typhimurium KCCM 40253 [57] and P. aeruginosa UFPEDA 39 [58] towards prodigiosin. However, MRSA and E. faecalis are more susceptible to prodigiosin compared to Salmonella Typhimurium and P. aeruginosa.…”

Section: S Marcescens Secretes Prodigiosin To Survive a Competitive Multispecies Environmentsupporting

confidence: 78%

“…Nonetheless, Salmonella Typhimurium resistance to prodigiosin appears to be more consistent. Salmonella Typhimurium SL1344 (this study), Salmonella Typhimurium KCTC 1926 [54] and Salmonella Typhimurium KCCM 40253 [57] are all resistant to prodigiosin's antibacterial activity. P. aeruginosa resistance towards prodigiosin could be attributed to the known production of rhamnolipids, a biosurfactant [60].…”

Section: Prodigiosin Selectively Antagonizes Gram-positive Bacteriamentioning

confidence: 71%

“…aeruginosa MCTC 1688 [ 59 ] and P . aeruginosa KCCM 11266 [ 57 ] are susceptible to prodigiosin. Nonetheless, Salmonella Typhimurium resistance to prodigiosin appears to be more consistent.…”

Section: Discussionmentioning

confidence: 99%

“…We also noted that P. aeruginosa PA14 and Salmonella Typhimurium SL1344 are resistant towards prodigiosin at both tested concentrations. While P. aeruginosa [9] and P. aeruginosa UFPEDA 39 [58] are also resistant to prodigiosin, P. aeruginosa MCTC 1688 [59] and P. aeruginosa KCCM 11266 [57] are susceptible to prodigiosin. Nonetheless, Salmonella Typhimurium resistance to prodigiosin appears to be more consistent.…”

Section: Prodigiosin Selectively Antagonizes Gram-positive Bacteriamentioning

confidence: 99%

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Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition

et al. 2021

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Prodigiosin, a red linear tripyrrole pigment, has long been recognised for its antimicrobial property. However, the physiological contribution of prodigiosin to the survival of its producing hosts still remains undefined. Hence, the aim of this study was to investigate the biological role of prodigiosin from Serratia marcescens, particularly in microbial competition through its antimicrobial activity, towards the growth and secreted virulence factors of four clinical pathogenic bacteria (methicillin-resistant Staphylococcus aureus (MRSA), Enterococcus faecalis, Salmonella enterica serovar Typhimurium and Pseudomonas aeruginosa) as well as Staphylococcus aureus and Escherichia coli. Prodigiosin was first extracted from S. marcescens and its purity confirmed by absorption spectrum, high performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrophotometry (LC-MS/MS). The extracted prodigiosin was antagonistic towards all the tested bacteria. A disc-diffusion assay showed that prodigiosin is more selective towards Gram-positive bacteria and inhibited the growth of MRSA, S. aureus and E. faecalis and Gram-negative E. coli. A minimum inhibitory concentration of 10 μg/μL of prodigiosin was required to inhibit the growth of S. aureus, E. coli and E. faecalis whereas > 10 μg/μL was required to inhibit MRSA growth. We further assessed the effect of prodigiosin towards bacterial virulence factors such as haemolysin and production of protease as well as on biofilm formation. Prodigiosin did not inhibit haemolysis activity of clinically associated bacteria but was able to reduce protease activity for MRSA, E. coli and E. faecalis as well as decrease E. faecalis, Salmonella Typhimurium and E. coli biofilm formation. Results of this study show that in addition to its role in inhibiting bacterial growth, prodigiosin also inhibits the bacterial virulence factor protease production and biofilm formation, two strategies employed by bacteria in response to microbial competition. As clinical pathogens were more resistant to prodigiosin, we propose that prodigiosin is physiologically important for S. marcescens to compete against other bacteria in its natural soil and surface water environments.

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Section: Prodigiosin Selectively Antagonizes Gram-positive Bacteriasupporting

confidence: 85%

Section: S Marcescens Secretes Prodigiosin To Survive a Competitive Multispecies Environmentsupporting

confidence: 78%

Section: Prodigiosin Selectively Antagonizes Gram-positive Bacteriamentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Prodigiosin Selectively Antagonizes Gram-positive Bacteriamentioning

confidence: 99%

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Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition

et al. 2021

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Valorization of Agri-Food Industry Waste for the Production of Microbial Pigments: An Eco-Friendly Approach

Kaur¹,

Singh²,

Ghoshal³

et al. 2022

Advances in Agricultural and Industrial Microbiology

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Enhanced production of prodigiosin by Serratia marcescens UCP 1549 using agrosubstrates in solid-state fermentation

et al. 2021

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Antimicrobial Activity of Prodigiosin from Serratia sp. PDGS120915 Against Intestinal Pathogenic Bacteria

Cited by 7 publications

References 10 publications

Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition

Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition

Valorization of Agri-Food Industry Waste for the Production of Microbial Pigments: An Eco-Friendly Approach

Enhanced production of prodigiosin by Serratia marcescens UCP 1549 using agrosubstrates in solid-state fermentation

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