Antimicrobial activity of propolis extract fractions against Staphylococcus spp. isolated from goat mastitis

Abstract

The indiscriminate use of antibiotics in the treatment of caprine mastitis causes the appearance of resistant microorganisms, besides leaving residues in milk, putting at risk to human health. In this way, propolis is an alternative in the treatment of diseases because it has antimicrobial activity, mainly because of the presence of flavonoids in its composition. The aim of this study was to evaluate the antimicrobial potential of propolis to Staphylococcus spp. Isolated from cases of goat mastitis and qualify the crude ethanoic extract by high performance liquid chromatography (HPLC). In this study, the minimum bactericidal concentration values of propolis extracts in ethanol, ethyl acetate and hexane showed that the best concentrations capable of promoting the highest mortality of the isolates of Staphylococcus spp. from mastitis in goats, were 6250, 3125 and 1562.5μg/mL, respectively. By the microplate adherence test, it was found that 20.78% isolates were not able to form biofilm, 14.70% were classified as moderate and 64.70% were weak and none as a strong biofilm producer. Propolis in its different diluents was able to affect the formation of biofilm and showed a pronounced marked antimicrobial activity against Staphylococcus spp. strains and may be indicated for use in in vivo studies.

The Protective Effect of Propolis Nanoemulsion (NEP) Against UVB Irradiation Induced Photoaging in Human Dermal Fibroblast (HDF)

“…The cells was treated with NEP in series of concentration (0 µg/ml, 0.1 µg/ml , 0.5 µg/ml, 1 µg/ml, 10 µg/ml, 25 µg/ml, 50 µg/ml, 100 µg/ml) with different incubation time. HDF cells were seeded in 96 well plates, with the total cell number was 5.000 cells/well and 3Bio Journal of Biological Science, Technology and Management 2(2): [1][2][3][4][5][6][7][8][9][10] Barlian et al incubated with complete growth medium containing NEP for 24 hours, 8 hours, and 1 hour. After incubation, cells were washed with Phosphate Buffer Saline (PBS) and incubated in growth medium for 24 hours.…”

“…Propolis contains several complex natural compounds, which have potential antioxidant properties such as flavonoids and polyphenols [6]. Several studies have mentioned the role of propolis as an anti-microbe [7], anti-inflammation [8], antitumor [9,10], and antioxidant [11]. Until now, there is no study that tests propolis as an anti-photoaging property in the human skin.…”

Extrato alcoólico de própolis no controle de verminoses em ovinos

“…Todavia, o uso indiscriminado de antibióticos, subdosagens, diagnósticos incorretos e falta de rotatividade de princípios ativos, têm provocado resistência dos parasitas a esses químicos (Goncuoglu et al, 2010;Gharsa et al, 2012;Shabana & Al-nazi, 2020) e o risco de transferência de resíduos para os produtos animais (Morsy et al, 2013). Desse modo, há uma necessidade de estudos de novas substâncias com capacidade anti-helmíntica e de caráter natural, que possam reduzir a resistência dos parasitas, a poluição ambiental e os resíduos dos produtos (Heinzen et al, 2012;Santos et al, 2019).…”

Natural Rubber Films Incorporated with Red Propolis and Silver Nanoparticles Aimed for Occlusive Dressing Application

“…Literature has reported the antibacterial activity of red propolis due to its complex and varying chemical composition 9 which includes flavonoids and aromatic acids, as well as different types of terpenoids 10,11 . It is constituted of exudates from floral shoots and buds of various plants, presenting many benefits to human health, like antioxidant, anti-inflammatory and regenerating properties [9][10][11][12] . Red propolis (RP) was chosen to be studied in this work, as it is a product that can be only found in Brazil, so it represents a native resource that can help in public health.…”