Antimicrobial Activity of Rhoeo discolor Phenolic Rich Extracts Determined by Flow Cytometry

Abstract

Abstract:Traditional medicine has led to the discovery of important active substances used in several health-related areas. Phytochemicals in Rhoeo discolor extracts have proven to have important antimicrobial activity. In the present study, our group determined the antimicrobial effects of extracts of Rhoeo discolor, a plant commonly used in Mexico for both medicinal and ornamental purposes. We evaluated the in vitro activity of phenolic rich extracts against specifically chosen microorganisms of human health importance by measuring their susceptibility via agar-disc diffusion assay and flow cytometry: Gram-positive Listeria innocua and Streptococcus mutans, Gram-negative Escherichia coli and Pseudomonas aeruginosa, and lastly a fungal pathogen Candida albicans. Ten different extracts were tested in eight different doses on all the microorganisms. Analytical data revealed a high content of phenolic compounds. Both agar-disc diffusion assay and flow cytometry results demonstrated that Pseudomonas aeruginosa was the least affected by extract exposure. However, low doses of these extracts (predominantly polar), in a range OPEN ACCESSMolecules 2015, 20 18686 from 1 to 4 μg/mL, did produce a statistically significant bacteriostatic and bactericidal effect on the rest of the microorganisms. These results suggest the addition of certain natural extracts from Rhoeo discolor could act as antibacterial and antimycotic drugs or additives for foods and cosmetics.

Neuroprotective effects of Tradescantia spathacea tea bioactives in Parkinson’s disease: In vivo proof-of-concept

“…According to the literature, extracts of T. spathacea leaves can be obtained by distinct methods, such as decoction 36 and by Soxhlet, 37 however, the overall extraction yields (2.8, 8.4% and 3.4%, respectively) were reported to be lower than those found in our study. Moreover, DTTS values of phenolic compounds and flavonoids were higher than others previously reported: TPC of 2.3 mg/g, 7 8.5 mg/g, 38 TFC of 0.7 mg/g, 7 and 10.8 mg/g. 4 …”

Activity of Zebrina Pendula Schnizl. Extract Against Staphylococcus Aureus, Candida Albicans, and Bacillus Subtilis

“…leaves is purple. This distinct coloration is attributed to the presence of anthocyanins, which are known for their potential to confer resistance to microbes (García-Varela et al, 2015). The appearance of the Zebrina pendula Schinzl plant is shown in Fig.…”

Bryo-Activities: A Review on How Bryophytes Are Contributing to the Arsenal of Natural Bioactive Compounds against Fungi

“…The flow cytofluorometric method is capable to discriminate three distinct subpopulations (dead, viable and injured cells), allowing one to evaluate the antifungal activity of the plant extract on the tested microorganism. Fungal spores suspension is mixed with an equal volume of plant extract and shook for the desired time, usually 25 °C for 24 h [ 224 ] or 10 min at room temperature [ 225 ]. Then, the cells are pelleted, resuspended in PI solution (25 µg/mL in phosphate-buffered saline) and incubated in the dark for 30 min at 32 °C.…”