Antimicrobial activity of sesquiterpene lactones isolated from traditional medicinal plant, Costus speciosus (Koen ex.Retz.) Sm

Duraipandiyan, Veeramuthu; Al-Harbi, Naif Abdullah; Ignacimuthu, Savarimuthu; Muthukumar, Chinnasamy

doi:10.1186/1472-6882-12-13

Cited by 86 publications

(59 citation statements)

References 31 publications

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“…S1D to F). Furthermore, oxygenous sesquiterpenoids that have been oxidized from oxygen-free sesquiterpenoids have stronger antimicrobial activity (46). These processes restrict the overgrowth of endophytes in planta (47) and are essential strategies used by host plants to modulate the growth of endophytes in vivo and to maintain a mutualistic relationship with endophytes.…”

Section: Discussionmentioning

confidence: 99%

Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea

Zhou

Yuan

et al. 2016

Appl Environ Microbiol

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bOxygenous terpenoids are active components of many medicinal plants. However, current studies that have focused on enzymatic oxidation reactions cannot comprehensively clarify the mechanisms of oxygenous terpenoid synthesis and diversity. This study shows that an endophytic bacterium can trigger the generation of reactive oxygen species (ROS) that directly increase oxygenous sesquiterpenoid content and diversity in Atractylodes lancea. A. lancea is a famous but endangered Chinese medicinal plant that contains abundant oxygenous sesquiterpenoids. Geo-authentic A. lancea produces a wider range and a greater abundance of oxygenous sesquiterpenoids than the cultivated herb. Our previous studies have shown the mechanisms behind endophytic promotion of the production of sesquiterpenoid hydrocarbon scaffolds; however, how endophytes promote the formation of oxygenous sesquiterpenoids and their diversity is unclear. After colonization by Pseudomonas fluorescens ALEB7B, oxidative burst and oxygenous sesquiterpenoid accumulation in A. lancea occur synchronously. Treatment with exogenous hydrogen peroxide (H 2 O 2 ) or singlet oxygen induces oxidative burst and promotes oxygenous sesquiterpenoid accumulation in planta. Conversely, pretreatment of plantlets with the ROS scavenger ascorbic acid significantly inhibits the oxidative burst and oxygenous sesquiterpenoid accumulation induced by P. fluorescens ALEB7B. Further in vitro oxidation experiments show that several oxygenous sesquiterpenoids can be obtained from direct oxidation caused by H 2 O 2 or singlet oxygen. In summary, this study demonstrates that endophytic bacterium-triggered ROS can directly oxidize oxygen-free sesquiterpenoids and increase the oxygenous sesquiterpenoid content and diversity in A. lancea, providing a novel explanation of the mechanisms of oxygenous terpenoid synthesis in planta and an essential complementarity to enzymatic oxidation reactions.A tractylodes lancea is a traditional Chinese medicinal plant and is the main ingredient of many famous Chinese medicines. Oxygenous sesquiterpenoids, such as hinesol, ␤-eudesmol, atractylone, and caryophyllene oxide, are the main active components in A. lancea and have medicinal efficacy against rheumatic diseases, digestive disorders, night blindness, and influenza (1). The quality of A. lancea strongly depends on the area in which the herb is produced, and A. lancea grown in the Maoshan area of the Jiangsu Province is the geo-authentic herb (2), which is characterized by higher oxygenous sesquiterpenoid content and diversity than herbs grown in other areas (3). In recent years, the geoauthentic A. lancea has become endangered due to habitat destruction and overexploitation. Although cultivation ensures the production of medicinal materials, the oxygenous sesquiterpenoid content and diversity in cultivated A. lancea is considerably decreased compared to that of the wild herb. Currently, knowledge on the biosynthesis and diversity of oxygenous sesquiterpenoids is insufficient. Some studies have explained...

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Section: Discussionmentioning

confidence: 99%

Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea

Zhou

Yuan

et al. 2016

Appl Environ Microbiol

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“…59, No.1A, pp: 38-43 04 existence of phenolic and alkaloid compounds. [2] Quercetin, Rutin, Luteolin, Kaempherol and Coumarin showed strong antibacterial activity in many studies [6]. …”

Section: Antibacterial Activity Testmentioning

confidence: 99%

“…Also herbal products are safer, less expensive and infrequently have side effects in comparison with synthetic drugs [1]. Among various herbals valued, C. speciosus is an erect herbaceous pharmacologically important one [2]. Costus speciosus is pharmaceutical and decorative plant cultivated in India crepe ginger belongs to family Costaceae (Zingiberaceae) [3].…”

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confidence: 99%

Evaluation of some bioactive effect of phenolic compounds in Costus speciosus rhizome extract

Thabit

2018

ijs

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This work was evaluated the antioxidant, antibacterial and cytotoxic activity of Costus speciosus rhizomes methanol extract. The FLC analysis showed the presence of five compounds in the methanol extract of C. speciosus rhizomes. These compounds were Quercetin (5.2mg/ml), Rutin (6.02mg/ml), Luteolin, (18.3mg/ml), Kaempherol, (11.34mg/ml) and Coumarin (1.41mg/ml). The maximum antioxidant activity of the extract was at concentration 1000 μg/ml with free radical scavenging activity approximately 67.5%. It was less than standard ascorbic acid 85.5% and Gallic acid 90% with significant difference (p≤0.05), with no significant difference in comparison with standard TBA 68.5%. The IC50 of extract was 3093μg/ml, while the IC50 of ascorbic acid, Gallic acid and TBA were (277.2, 364.5 and 601.3 μg/ml) respectively. The extract revealed influential growth inhibition for all bacteria used in this experiment. The extract was moderately effective at concentration 400 μg/ml of extract with inhibitory activity 50.7 % on MCF-7 cell line and IC50 139.1 μg/ml. Keywords ISSN: 0067-2904Thabit Iraqi Journal of Science, 2018, Vol. 59, No.1A, pp: 38-43 93 IntroductionFrom early years, there is a virtual increasing interest in formation of drugs derived from herbes which can be used as alternative therapy. Also herbal products are safer, less expensive and infrequently have side effects in comparison with synthetic drugs [1]. Among various herbals valued, C. speciosus is an erect herbaceous pharmacologically important one [2]. Costus speciosus is pharmaceutical and decorative plant cultivated in India crepe ginger belongs to family Costaceae (Zingiberaceae) [3]. Costus speciosus have traditional uses such as food and medicine [4]. Recently the juice of rhizome therapeutically implemented as cooling and relief from head-ache, powder of leaves implemented as antipyretic, the extract acquired by boiling stem part of this plant is used against dysentery and fever, and many cures against diarrhea [5] cough, cuts, burning sensation, scabies, arthritis [6], wounds, constipation, leprosy, for abortion, asthma, inflammations, anemia [7] intestinal pains, nose pain , rash, worm infection, to stop vomiting [8] spermatorrhoea [9] used as antivermin and skin diseases [10]. Earlier studies paid attention on antioxidant, cytotoxicity and antimicrobial effects of plant extracts. On the other hand the diagnosis of compounds also had extensive studies to estimate the effect of these compounds [11]. Hence the aimes of the present study were to determine in vitro antioxidant, antibacterial and cytotoxic activity of phenolic compounds in methanol extract of C. speciosus rhizomes. Materials and methods Plant materialThe rhizome of C. speciosus was obtained from herbalists market in Baghdad region and was identified by the Faculty of Agriculture, University of Baghdad. Plant extractionThe sundried rhizomes were powdered with grinder and then passed through sieve with 40 meshes. About 25g of dried rhizomes powder subjected to Soxhlet apparatus and...

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“…Saussurea costus is considered to be one of the antioxidant-rich medicinal plants. Many authors have reported that the roots of this plant possess various biological activities, including cortisol lowering effect [38], anti-infammatory [39,40], anticancer [41,42], antiviral [23], antimicrobial [43,44], antifungal [15], antioxidant [45,46], antidiabetic [47], antiulcer [20] and hepatoprotective effects [38]. Liv-52 is widely used in treatment of liver diseases of varying origins.…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective Effect of Costus Roots Extract against Carbon Tetrachloride (CCl4)-Induced Liver Injury in Guinea Pigs

Al-Duais¹,

Al-Awthan²

2017

JLS

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SC (Saussurea costus) (Asteraceae) is a traditionally known and potent plant which is well considered for its medicinal uses in different indigenous Indian systems of medicine. This study was undertaken to investigate the hepatoprotective effect of SC against liver injury induced by CCl 4 intoxication. Animals were divided into five groups. Group I, served as normal control. Group II received 2 mL CCl 4 /kg b.w. diluted with olive oil, at 1:1 ratio on day 11. Groups III and IV were pre-treated orally respectively with 100 and 200 mg/kg b.w. aqueous roots extract of SC for 10 days followed by subcutaneous injection of CCl 4 (2 mL/kg b.w.), once on day 11. Group V were orally given Liv-52 (100 mg/kg b.w.) once daily for 10 days followed by subcutaneous injection of CCl 4 (2 mL/kg b.w.), once on day 11. Our results show that, the activity of serum hepatic enzymes (ALT, AST, and ALP) were significantly elevated in guinea pigs treated with CCl 4 , while both the SC root extract and Liv-52 reduced significantly these enzymes activity. Also, the levels of glucose, urea and cholesterol were decreased when compared with intoxicated control. Histopathological examination of intoxicated animals showed dilation, hemorrhage, vacuolization, inflammation and necrosis indicating liver damage, while the animals received SC or Liv-52 that showed less pathological effects or normal liver when compared to animals treated with CCl 4 alone. Biochemical and histological results confirm the hepatoprotective effect of aqueous extract of SC.

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Antimicrobial activity of sesquiterpene lactones isolated from traditional medicinal plant, Costus speciosus (Koen ex.Retz.) Sm

Cited by 86 publications

References 31 publications

Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea

Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea

Evaluation of some bioactive effect of phenolic compounds in Costus speciosus rhizome extract

Hepatoprotective Effect of Costus Roots Extract against Carbon Tetrachloride (CCl4)-Induced Liver Injury in Guinea Pigs

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