Antimicrobial Activity of Several Calcium Hydroxide Preparations in Root Canal Dentin

Abstract

The purpose of this study was to evaluate the antimicrobial activity of several calcium hydroxide (Ca(OH)2) preparations in root canal dentin infected with Enterococcus faecalis. Roots of extracted bovine incisors were prepared to standardized cylindrical test specimens of 5 mm in height; the smear layer was removed, and the specimens were incubated for 24 h at 37 degrees C in bacteriological culture medium that contained 7.0 x 10(4) colony forming units per milliliter of E. faecalis. The specimens were mounted in individual 4-mm diameter culture wells, and the test material was applied to fill the canal lumen. There were five treatment groups: group 1, a thick mixture of Ca(OH)2 USP (1.0 g/ml H2O); group 2, a thin mixture of Ca(OH)2 USP (0.1 g/ml H2O); group 3, Pulpdent TempCanal paste; group 4, sterile H2O (positive control); and group 5, 25 dentin specimens in sterile, uninoculated brain-heart infusion broth that were included as negative controls. Quantitative microbiological analysis of dentin at various depths was completed after 24 h. All groups showed a significant (p < 0.001) decrease in numbers of E. faecalis in all depths of dentin compared with the control. Groups 2 and 3 demonstrated significantly greater antimicrobial activity (73%-86% reduction) at all depths of dentin tested compared with group 1 (13%-26%) (p < 0.05). These results suggest that Ca(OH)2 can decrease the numbers of E. faecalis at all depths of dentinal tubules within 24 h and that thin preparations of Ca(OH)2 may be more effective in the elimination of E. faecalis from dentinal tubules than thick preparations.

Direct and indirect antibacterial effects of various concentrations of triple antibiotic pastes loaded in a methylcellulose system

“…methylcellulose system resulted in significantly better antibacterial effects against various endodontic pathogens in comparison to higher concentrations of Ca(OH) 2 pastes prepared without methylcellulose (30,31). It is also worth noting that methylcellulose is frequently used as culture media for stem cell growth and differentiation due to its noncytotoxic nature (32).…”

Measurement of pH and calcium ions release from different calcium hydroxide pastes at different intervals of time: Atomic spectrophotometric analysis

“…7 Though most of the studies have been conducted to study the release of OH ions and their effect on periapical pH and repair, a very few discuss about the Ca ion release and its role in periapical healing. 8,9 Therefore, this study was conducted to determine the effect of various vehicles and delivery techniques on the pH of the paste and release of Ca ions from the apical one-third of the root canals using an atomic spectrophotometer.…”

Alternati̇f İrri̇gasyon Solüsyonu Olarak Gümüş Nanoparçaciklarin E. Faecali̇s Üzeri̇ne Anti̇bakteri̇yel Etki̇si̇

“…19,23 İrrigasyon solüsyonlarının antibakteriyel etkinliklerinin belirlenmesinde koloni oluşturan birim sayma yöntemi geleneksel olarak ve yaygın bir biçimde kullanılan mikrobiyolojik bir yöntemdir. 16,21,23 Bu yöntemin kullanıldığı çalışmalarda kullanılan besiyeriyle irrigasyon solüsyonlarının arasındaki kimyasal etkileşimin bilinmemesi bu yöntemin kısıtlılığıdır. Bu çalışmada, bu etkileşimin azaltılması amacıyla inkübasyonu takiben, kök kanalları mevcut besiyerini uzaklaştırmak için 5 mL serum fizyolojik ile yıkandı.…”