Antimicrobial activity of stigmasterol from the stem bark of &lt;i&gt;Neocarya macrophylla&lt;/i&gt;

Yusuf, A. J.; Abdullahi, M. I.; Aleku, Godwin A.; Ibrahim, Ilyasu A.A.; Alebiosu, Celestina O.; Yahaya, Maryam; Adamu, Hajara W.; Sanusi, A.; Mailafiya, Maria M.; Abubakar, H.

doi:10.4102/jomped.v2i1.38

Cited by 37 publications

(31 citation statements)

References 18 publications

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“…The anti-tubercular activity of oleanonic acid (1) against Mycobacterium tuberculosis was previously reported [18]. β-stigmasterol and βsitosterol (3a/b) have demonstrated broad spectrum antibacterial activity against several pathogenic bacteria in previous studies [29][30][31][32]. This is the first report on the antibacterial activity of 4-hydroxy cinnamic acid docosyl ester (2) though some cinnamic acid derivatives have been shown to possess very potent antibacterial activity [33].…”

Section: Discussionmentioning

confidence: 85%

Bioactive constituents with antibacterial, resistance modulation, anti-biofilm formation and efflux pump inhibition properties from Aidia genipiflora stem bark

et al. 2021

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Background Antimicrobial resistance is a global health challenge. The involvement of bacterial biofilms and efflux pumps in the development of multidrug resistance (MDR) is well established. Medicinal plants have been proposed as alternatives for combating MDR focusing on their bioactive constituents with resistance modulatory activities. This study was aimed at investigating the stem bark of Aidia genipiflora for bioactive constituents with anti-biofilm, efflux pump inhibition and resistance modulatory activities. Method The crude methanol extract was purified by column chromatography and isolated compounds characterized by mass and nuclear magnetic resonance spectrometry. Antibacterial activity was determined by the High-throughput spot culture growth inhibition and the broth micro-dilution assay. The ethidium bromide accumulation assay was used to determine efflux pump inhibition property. Biofilm inhibition was determined in a microplate crystal violet retention assay. Results Purification of the ethyl acetate fraction led to the isolation of oleanonic acid (1), 4-hydroxy cinnamic acid docosyl ester (2), β-stigmasterol/β-sitosterol (mixture 3a/b) and D-mannitol (4). The minimum inhibitory concentrations (MICs) ranged from 250 to > 500 μg/mL for extracts and fractions and from 15 to 250 μg/mL for compounds. In the presence of sub-inhibitory concentrations of the compounds, the MIC of amoxicillin against E. coli (20 μg/mL) and P. aeruginosa (320 μg/mL) was reduced by 32 and 10 folds respectively. The whole extract demonstrated anti-biofilm formation and efflux pump inhibition in E. coli, S. aureus and P. aeruginosa. The sterol mixture (3a/b) at concentration of 100 μg/mL caused the highest inhibition (73%) of biofilm formation in S. aureus. Oleanonic acid (1) demonstrated remarkable efflux pump inhibition at MIC of 7.8 μg/mL in E. coli better than the standard drugs verapamil and chlorpromazine. Conclusion This study confirms the prospects of A. genipiflora as a source of new antibacterial agents and adjuvants that could interact with some resistance mechanisms in bacteria to enhance the activity of hitherto ineffective antibiotics. “A small portion of the study has been presented in a conference in the form of poster”.

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Section: Discussionmentioning

confidence: 85%

Bioactive constituents with antibacterial, resistance modulation, anti-biofilm formation and efflux pump inhibition properties from Aidia genipiflora stem bark

et al. 2021

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“…Likewise, for the direct extract fractions of EA, only 5 out of 8 compounds detected (with area % scores above 0.5%) were known to possess such activity. These are DGEA1 19 , DGEA2 20 , DGEA3 21 , DGEA4 22 and DGEA5 23 . However, for the AC extract fraction, 4 out of total 6 compounds detected, were reported to possess the antibacterial effect.…”

Section: Discussionmentioning

confidence: 99%

Chromatographic Analyses, Virtual Screening and Pharmacokinetics of Yellow Malaysian Rambutan (Nephelium lappaceum L.) Fruit Epicarp Extracts Reveal Potential Antibacterial Compounds

Asghar¹,

Tan²,

Zahoor³

et al. 2020

Preprint

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The emergence and spread of antimicrobial resistance have been of serious concern on human health and the management of bacterial infectious diseases. Effective treatment of these diseases requires the development of novel therapeutics, preferably free of side effects. In this regard, natural products are frequently conceived to be potential alternative sources for novel antibacterial compounds. Herein, we have evaluated the antibacterial activity of the epicarp extracts of the Malaysian cultivar of yellow rambutan fruit (Nephelium lappaceum L.) against six pathogens namely, Bacillus subtilis, methicillin-resistant Staphylococcus aureus (MRSA), Streptococcus pyogenes, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella enterica. Among a series of solvent extracts, fractions of ethyl acetate and acetone have revealed significant activity towards all tested strains. Chemical profiling of these fractions, via HPLC, LC-MS and GC-MS, has generated a library of potential bioactive compounds. Downstream virtual screening, pharmacological prediction, and receptor-ligand molecular dynamics simulation have eventually unveiled novel potential antibacterial compounds, which can be extracted for medicinal use. To this end, we report novel mechanistic aspects of these compounds in competitively inhibiting the ATP-binding domain of the DnaK chaperone of P. aeruginosa and MRSA. Our work takes a step forward to discover antimicrobials capable of perforating the barrier of resistance posed by both the gram positives and the negatives.

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“…The ease of availability of this plant in Nigeria and elsewhere prompted us to conduct antisnake venom activity and phytochemical analysis of the stem bark of N. macrophylla in which stigmasterol and bis-(5,7-diacetyl-catechin-4'-αrhamnopyranoside) were isolated (Yusuf et al, 2015a;Yusuf et al, 2015b). Indeed, we have recently demonstrated the antimicrobial activity of stigmasterol isolated from N. macrophylla, exhibiting broad spectrum antimicrobial activity thereby validating its use for the treatment of infectious diseases (Yusuf et al, 2018).…”

Section: Introductionmentioning

confidence: 89%

Antisnake venom activity and isolation of quercetin from the leaf of Neocarya macrophylla (Sabine) Prance ex F. White (Malpighiales: Chrysobalanaceae)

Yusuf¹,

Abdullahi²,

Musa³

et al. 2019

Braz. J. Biol. Sci.

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Snake envenomation is a major cause of death and disability in many developing countries. Neocarya macrophylla (Sabine) Prance ex F. White (Malpighiales: Chrysobalanaceae) have been reportedly used in traditional medicine to treat snake envenomation. Bioassay-guided isolation of antivenom principles was carried out on the leaf of N. macrophylla against Naja nigricollis venom. The methanol extract of N. macrophylla leaf and its ethylacetate and n-butanol fraction significantly (P < 0.05) protected mice against venom-induced lethality with 100% survival rate and there was remarkable inhibition of the poisonous effects of PLA2 enzyme by the extracts and the fractions. Encouraged by this result, the ethylacetate soluble fraction was subjected to purification using vacuum liquid chromatography and gel filtration which led to the isolation of quercetin as the bioactive principle. The identity of the compound was determined on the basis of chemical tests, and by comparison of its 1H-NMR data with literature, this is the first report of isolation of this compound from the leaf of the plant. However, the results of the study suggests that the leaf of N. macrophylla possess significant antisnake venom activity which provide the scientific basis for its use in traditional treatment of snakebites.

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Antimicrobial activity of stigmasterol from the stem bark of <i>Neocarya macrophylla</i>

Cited by 37 publications

References 18 publications

Bioactive constituents with antibacterial, resistance modulation, anti-biofilm formation and efflux pump inhibition properties from Aidia genipiflora stem bark

Bioactive constituents with antibacterial, resistance modulation, anti-biofilm formation and efflux pump inhibition properties from Aidia genipiflora stem bark

Chromatographic Analyses, Virtual Screening and Pharmacokinetics of Yellow Malaysian Rambutan (Nephelium lappaceum L.) Fruit Epicarp Extracts Reveal Potential Antibacterial Compounds

Antisnake venom activity and isolation of quercetin from the leaf of Neocarya macrophylla (Sabine) Prance ex F. White (Malpighiales: Chrysobalanaceae)

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