Antimicrobial Activity of the Leaves of Endemic Stachys pseudopinardii in Turkey

“…The bacterial species used in the present study were carefully selected because of their clinical importance, especially with the current increasing trends of multidrug resistance among emerging and re-emerging bacterial pathogens to the many available different antibiotics with their frequent uses. Recent scientific research has shown that many plants used in traditional medicine are potentially toxic, allergic, mutagenic, and/or carcinogenic (Ahmad et al, 1998;Dulger and Aki, 2009). Therefore, in vitro cytotoxic evaluation studies are important to obtain effective safe new gents that have certain desirable properties such as: little or no toxic effects on normal cells, high efficacy on multiple sites, capability of oral consumption, known mechanism of action, low cost, and acceptance in the community (Aziz et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…All the tubes were then incubated at 37°C for 24 hrs and then examined for growth by observing turbidity. The minimum bactericidal concentration (MBC) of the plant extract on the tested bacterial species was carried out according to (Ajaiyeoba et al, 2003;Dulger and Aki, 2009). Briefly, 1ml from the MIC tubes showing no growth was subcultured on to MHA plate and incubated at 37°C for 24 hrs.…”

Section: Determination Of Minimum Inhibitory Concentration (Mic) and mentioning

confidence: 99%

In Vitro Cytotoxicity and Antibacterial Evaluation of Aqueous, Methanolic and Ethanolic Extracts of Anastatica hierochuntica against Pathogenic Bacteria

Sobeai¹

2016

Int. J. Curr. Res. Biosci. Plant Biol.

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A b s t r a c t A r t i c l e I n f oThe cytotoxic effect of Anastatica hierochuntica was evaluated in vitro using Vero cell lines, characterized by identification of cellular alterations microscopically and cellular viability colorimetrically. The mean CC 50 value of methanol, ethanol, and aqueous extract were lower than 1000 µg/ml. The antibacterial activity of ethanol, methanol and aqueous extracts of A. hierochuntica was evaluated using agar well diffusion method against 10 bacterial pathogens. All the extracts showed substantial antibacterial activity against the selected pathogens. The methanolic extract showed highest inhibition zone followed by the aqueous and ethanolic extract. Among the bacterial pathogens, L. monocytogenes was more resistant towards and ethanol extract. Although, other tested bacteria were susceptible towards aqueous ethanol and methanol extract. However, methanol extract was effective against the tested pathogens, when compared with the other extracts. The minimum inhibitory concentrations (MIC) ranged from 15-50 mg/ml to inhibit the growth of the tested bacteria. The results show that the plant contains bioactive components which can inhibit the bacterial pathogens which can be further purified and used against the multi-drug resistant bacteria.

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“…The MIC is determined for the highly active plant that showed significant antibacterial activity against the test bacteria according to the methods of Nakamura et al (1999) and Dulgar and Aki (2009) with some modifications. Serial dilution of each of the extracts were prepared using the 5% DMSO (dimethyl sulphoxide) to produce the final concentrations of 100, 50, 25,12.5, 6.25 and 3.125%.…”

Section: Determination Of Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

Antibacterial activity of some selected plants traditionally used as medicine in Manipur

Ishwori¹,

Talukdar²,

Singh³

et al. 2014

Afr. J. Biotechnol.

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Methanolic leaf extracts of the plant species Elsholtzia blanda Benth., Elsholtzia communis (Collett & Hemsl) Diels., Polygonum posumbu Buchanam-Hamilton ex D. Don and Zanthoxylum acanthopodium DC. using methanol as a solvent were tested against 10 human pathogenic bacteria for potential antibacterial activity. The study revealed that all extracts show varied degree of antibacterial activity against the tested bacterial pathogens. The antibacterial activity was determined using agar well diffusion method. Methanolic extract of the leaf Zanthoxylum acanthopodium DC. showed antibacterial activity against five bacterial strains from among the ten bacteria tested followed by Polygonum posumbu, Elsholtzia communis and Elsholtzia blanda. Clostridium sporogenes was found to be susceptible to all the plants tested. Minimum inhibitory concentration of the plants against the tested organism ranged between 3.125-12.5 mg/ml. Hence these plants can be used to discover bioactive natural products that may serve as leads in the development of the new pharmaceuticals.

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Antimicrobial Activity of the Leaves of Endemic Stachys pseudopinardii in Turkey

Cited by 17 publications

References 13 publications

Evaluation of the Antibacterial Activity of 14 Medicinal Plants in Côte d’Ivoire

Evaluation of the Antibacterial Activity of 14 Medicinal Plants in Côte d’Ivoire

In Vitro Cytotoxicity and Antibacterial Evaluation of Aqueous, Methanolic and Ethanolic Extracts of Anastatica hierochuntica against Pathogenic Bacteria

Antibacterial activity of some selected plants traditionally used as medicine in Manipur

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