Antimicrobial activity-specific to Gram-negative bacteria and immune modulation-mediated NF-κB and Sp1 of a medaka β-defensin

Zhao, Jianmin; Zhou, Li; Jin, Junyan; Zhao, Zhe; Lan, Jing; Zhang, Yibin; Zhang, Qi-Ya; Gui, Jian‐Fang

doi:10.1016/j.dci.2008.11.006

Cited by 70 publications

(64 citation statements)

References 52 publications

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“…The Gig2-His-Tag fusion protein was then produced and purified by affinity chromatography on NiSO 4 -Sepharose. The purified protein was applied to immunize white rabbit to raise a polyclonal anti-Gig2 antiserum according to a previous report (Zhao et al, 2009). Prokaryotic expression and purification of crucian carp IFN (AY452069) was conducted using the same method as Gig2.…”

Section: Prokaryotic Expression Polyclonal Anti-gig2 Rabbit Antiserumentioning

confidence: 99%

Expression regulation and functional characterization of a novel interferon inducible gene Gig2 and its promoter

Jiang

Zhang

et al. 2009

Molecular Immunology

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Section: Prokaryotic Expression Polyclonal Anti-gig2 Rabbit Antiserumentioning

confidence: 99%

Expression regulation and functional characterization of a novel interferon inducible gene Gig2 and its promoter

Jiang

Zhang

et al. 2009

Molecular Immunology

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“…Defensins from teleost fishes have been identified by molecular methodologies rather than purification of the native peptides in several fish species [112,[177][178][179][180][181]. Zhou et al [179] used EST and complete genome data to identify defensins from zebrafish (Dario rerio) and pufferfish (Takifugu rubripes) that resemble the β-defensins of birds and mammals.…”

Section: Antimicrobial Peptidesmentioning

confidence: 99%

An Overview of the Immunological Defenses in Fish Skin

2012

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The vertebrate immune system is comprised of numerous distinct and interdependent components. Every component has its own inherent protective value, and the final combination of them is likely to be related to an animal's immunological history and evolutionary development. Vertebrate immune system consists of both systemic and mucosal immune compartments, but it is the mucosal immune system which protects the body from the first encounter of pathogens. According to anatomical location, the mucosa-associated lymphoid tissue, in teleost fish is subdivided into gut-, skin-, and gill-associated lymphoid tissue and most available studies focus on gut. The purpose of this paper is to summarise the current knowledge of the immunological defences present in skin mucosa as a very important part of the fish immune system, serving as an anatomical and physiological barrier against external hazards. Interest in defence mechanism of fish arises from a need to develop health management tools to support a growing finfish aquaculture industry, while at the same time addressing questions concerning origins and evolution of immunity in vertebrates. Increased knowledge of fish mucosal immune system will facilitate the development of novel vaccination strategies in fish.

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“…In human and mouse, more than 30 while in birds 14 b-defensins have been identified [13][14][15][16]. There are few fish species including Danio rerio (zfDB1-3) [1], Tetraodon nigroviridis (tnDB 1 and 2) [1], Takifugu rubripes (fuDB) [1], Oncorhynchus mykiss (omDB [1][2][3][4] [17,18], medaka (mkDB) [19], Epinephelus coioides (ogDB) [20], Paralichthys olivaceus of (DBI 1-5) [21] where single and multiple isoforms of b-defensin have been reported.…”

Section: Introductionmentioning

confidence: 98%

Molecular Characterization and In Silico Analysis of Defensin From Tor putitora (Hamilton)

Chaturvedi

Dhanik

Pande

2015

Probiotics & Antimicro. Prot.

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Antimicrobial peptides (AMPs) are vital constituents of innate immune system. In fish, one of the most important AMP is β-defensin that has a potential to activate the adaptive immune system. β-defensin is a cysteine-arginine-rich cationic AMP with broad spectrum activity against microbes. In this study, we identified cloned and characterized β-defensin 1 from Tor putitora, a cold water fish species also known as mahseer. An open reading frame of β-defensin 1 was amplified, cloned and sequenced which encodes a peptide of 67 amino acid residues. The pro-peptide includes a signal peptide comprising 24 amino acids as predicted by Signal P along with a mature peptide of 43 amino acid residues. Tor putitora β-defensin 1 (TP-βdf1) has a molecular weight of 4.6 kDa with a pI of 8.35. Six cysteine residues are present in the mature peptide which is a characteristic feature of defensins. All six cysteine residues are involved in the formation of three intra-molecular disulfide bonds. Three-dimensional modeling of mature peptide of TP-βdf1 was carried out using Modeller 9.10, and validated TP-βdf1 model revealed three β-sheets. The cysteine residues form three disulfide bonds in the pattern of Cys(1)-Cys(5), Cys(2)-Cys(4), Cys(3)-Cys(6) stabilizing the β-sheet. Structural analysis revealed three β-strands and an α-helix at the N terminus. Phylogenetic analysis revealed that the β-defensin 1 of Tor putitora was close to Megalobrama amblycephala which possibly suggests that TP-βdf1 peptide sequence is quite similar to β-defensin peptide sequences of carps and minnows.

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Antimicrobial activity-specific to Gram-negative bacteria and immune modulation-mediated NF-κB and Sp1 of a medaka β-defensin

Cited by 70 publications

References 52 publications

Expression regulation and functional characterization of a novel interferon inducible gene Gig2 and its promoter

Expression regulation and functional characterization of a novel interferon inducible gene Gig2 and its promoter

An Overview of the Immunological Defenses in Fish Skin

Molecular Characterization and In Silico Analysis of Defensin From Tor putitora (Hamilton)

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