Antimicrobial and acetylcholinesterase inhibitory activities of Buddleja salviifolia (L.) Lam. leaf extracts and isolated compounds

Pendota, S.C.; Aderogba, M.A.; Ndhlala, A.R.; Staden, J. Van

doi:10.1016/j.jep.2013.04.047

Cited by 52 publications

(33 citation statements)

References 30 publications

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“…Phytochemical analysis of Buddleja extracts identified polyphenols, flavonoids and phenylethanoid as major components [45, 46]. Buddleja showed antimicrobial activity against Bacillus subtilis, S. aureus, E. coli and K. pneumoniae supporting the traditional use of the plant in the treatment of eye infections [28]. In vivo studies in animal models showed that eye drops containing Buddleja can be used safely to treat dry eye [27, 47].…”

Section: Discussionmentioning

confidence: 99%

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Prevention of Bacterial Biofilm Formation on Soft Contact Lenses Using Natural Compounds

El-Ganiny

Shaker

Aboelazm

et al. 2017

J Ophthal Inflamm Infect

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PurposeIn eye care field, contact lenses (CL) have a great impact on improving vision, but their use can be limited by ocular infection. CL- associated infections can be reduced by good attention to CL storage case practice. CL-care solutions should be able to control microbial growth on CL.The aim of the study was to evaluate and compare the efficacy of CL-care solutions (found in Egyptian market) with some natural compounds in removal and inhibition of bacterial biofilm formed on soft CL.Clinical isolates were recovered from patients having conjunctivitis from Benha University Hospital and identified microbiologically. Quantification of biofilm was done using microtiter plate assay. Three multipurpose CL-care solutions were examined for their ability to remove and inhibit biofilm. Also four natural extracts having antibacterial activity and are safe on eye were tested for their anti-biofilm activity.ResultsThe major bacterial isolates from eye infections were Pseudomonas aeruginosa (36%) and Staphylococcus spp. (37.8%). Only 33.3% of isolates showed ability to produce weak to moderate biofilm. The tested multi-purpose CL-care solutions showed moderate ability to remove preformed biofilm. Among the tested natural compounds, Calendula officinalis and Buddleja salviifolia extracts showed an excellent efficacy in inhibition of biofilm and also removal of preformed biofilm.ConclusionThis study demonstrated that isolates from infected eye and CL-cases showed weak to moderate biofilm formation. Calendula officinalis and Buddleja salviifolia extracts showed excellent effect on inhibition and removal of biofilm, these extracts could be added into CL-care solutions which could markedly reduce eye-infections during CL-wear.

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Section: Discussionmentioning

confidence: 99%

“…Buddleja salviifolia leaves were used for treatment of eye infections by tribes in South Africa. The extract of Buddleja officinalis leaves was used for partial treatment for surface diseases of eyes, it also exhibited a broad spectrum antibacterial activity [27, 28]. …”

Section: Introductionmentioning

confidence: 99%

Prevention of Bacterial Biofilm Formation on Soft Contact Lenses Using Natural Compounds

El-Ganiny

Shaker

Aboelazm

et al. 2017

J Ophthal Inflamm Infect

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“…Anti-inflammatory activity of some Buddleja species (Jensen, 2000;Pendota et al, 2013, Pendota et al, 2014 has been previously described; this biological activity was tested in preclinical murine models of acute inflammation induced by Carrageenan and skin wounds induced by surgical injury. The results showed that the aqueous and methanolic (MeOH) extracts obtained from whole wild plants (Wp) of B. globosa (Backhouse et al, 2008), B. crispa (Bukhari et al, 2016), and B. polystachia (Al-Ati et al, 2015) showed an important inhibitory effect.…”

Section: Introductionmentioning

confidence: 99%

In vivo ANTI-INFLAMMATORY ACTIVITY AND ACUTE TOXICITY OF METHANOLIC EXTRACTS FROM WILD PLANT LEAVES AND CELL SUSPENSION CULTURES OF Buddleja cordata Kunth (Buddlejaceae)

et al. 2017

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Buddleja cordata is a species used by Mexican folk medicine for treating illnesses related to inflammation such as skin wounds and arthritis. It bio-synthesizes metabolites such as verbascoside that contributes to its ethno-therapeutic properties as antiinflammatory remedy. HPLC analysis showed that the methanolic extract from cell suspension cultures (Bc-Cc) and wild plant leaves (Bc-Wp) contained verbascoside, but concentration was higher in Bc-Cc (87.48 mg/g) than in wild plant (47.34 mg/g).In the acute toxicity model, none of the extracts generated any lethality or adverse effects. In acute inflammation model induced with TPA, Bc-Cc extract showed a greater edema inhibition at 2 mg/ear (61.72%), as well for carrageenan model at 200 mg/kg (48.87%). Bc-Wp showed lesser anti-inflammatory effect in both acute inflammation models than Bc-Cc. For Adjuvant-induced arthritis both extracts at 250 mg/kg generated a moderate inhibition over edema (≈ 33%) at day 28, and they were statistically no different to phenilbutazone. The culture in suspension of B. cordata obtained by biotechnological process contains greater amount of verbascosido and showed better anti-inflammatory activity; thus, representing a source for obtaining this type of secondary metabolite of pharmacological interest. Keywords: cell suspension culture, Buddleja cordata, verbascoside, anti-inflammatory activity, median lethal dose. ResumenBuddleja cordata es una planta medicinal usada en México para el tratamiento de enfermedades relacionadas con heridas en la piel y artritis. Esta especie bio-sintetiza metabolitos secundarios como el verbascósido, el cual contribuye a sus propiedades etno-medicinales. El análisis por HPLC mostró que el extracto metanólico del cultivo en suspensión celular (Bc-Cc) y de las hojas de la planta silvestre (Bc-Wp) contienen verbascósido, siendo su concentración mayor en el cultivo Bc-Cc (87.48 mg/g) que en la planta silvestre (47.34 mg/g). Ninguno de los extractos generó letalidad o efectos adversos en el ensayo de toxicidad aguda. En el modelo de inflamación tópica inducido con TPA, Bc-Cc mostró mayor actividad antiinflamatoria; a 2 mg/oreja generó 61.72% de inhibición y en el modelo de carragenina a 200 mg/kg generó 48.87%, mientras que Bc-Wp mostró menor actividad anti-inflamatoria en ambos modelos. En el modelo de artritis inducida con Adyuvante de Freud completo, ambos extractos a 250 mg/kg mostraron moderada actividad anti-inflamatoria, con inhibición de aprox. 33% al día 28. El cultivo en suspensión celular de B. cordata obtenido por proceso biotecnológico contiene mayor cantidad de verbascosido y mostró mejor actividad anti-inflamatoria; por lo que representa una fuente para la obtención de este tipo de metabolito secundario de interés

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“…Phytoalexins from medicinal plants are naturally defensive compound for all kinds of living organism and helpful in treatment, prevention and control of multidrug resistance strains of bacteria and fungi extracted from medicinal plants [5]. In present research, the R. succadanea crude extracts were explored for antibacterial potential against different strains of bacteria.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity of Rhus succedanea Var. Himalaica

Khan

Ibrar

Barkatullah

et al. 2017

PAB

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The antibacterial activity of leaf and root ethanolic extracts as well as crude alkaloids and flavonoids from both the parts of Rhus succedanea were carried against bacterial strains viz. Staphylococcus aureus, Clavibacter michiganensis, Proteus vulgaris and Xanthomonas spp. The antibacterial activity was carried out on nutrient agar medium using disk diffusion method. The flavonoid fraction of root showed significant antibacterial activity against Staphylococcus aureus, Clavibacter michiganensis, Proteus vulgaris and Xanthomonas spp with 24, 25, 15 and 23 mm zone of inhibition respectively; followed by leaf flavonoid fractions with 22, 18, 19 and 20 mm zone of inhibition at 1000µg/ml as compared to reference drug (streptomycin) having 27, 26, 26 and 26 mm at concentration of 0.5 µg/ml respectively. While ethanolic extract and other fractions showed dose dependent activities in order of highest root alkaloids < leaf alkaloids < leaf ethanolic extract and lowest by root ethanolic extract. MIC value confirmed that both alkaloids and flavonoids fraction were more potent than ethanolic extracts of root and leaves. The present study showed that R. succedanea have good significant antimicrobial potential against both gram positive and gram negative bacterial strains.

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Antimicrobial and acetylcholinesterase inhibitory activities of Buddleja salviifolia (L.) Lam. leaf extracts and isolated compounds

Cited by 52 publications

References 30 publications

Prevention of Bacterial Biofilm Formation on Soft Contact Lenses Using Natural Compounds

Prevention of Bacterial Biofilm Formation on Soft Contact Lenses Using Natural Compounds

In vivo ANTI-INFLAMMATORY ACTIVITY AND ACUTE TOXICITY OF METHANOLIC EXTRACTS FROM WILD PLANT LEAVES AND CELL SUSPENSION CULTURES OF Buddleja cordata Kunth (Buddlejaceae)

Antibacterial activity of Rhus succedanea Var. Himalaica

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