Antimicrobial and anti-biofilm activity of thymoquinone against Shigella flexneri

Fan, Qiuxia; Yuan, Yahong; Jia, Hang; Zhang, Xuejun; Wang, Zhouli; Hu, Zhongqiu; Gao, Zhenpeng; Yue, Tianli

doi:10.1007/s00253-021-11295-x

Cited by 17 publications

(11 citation statements)

References 31 publications

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“…4 Shigellosis, the cause of bacillary dysentery caused by S. flexneri, is the second leading cause of diarrheal deaths (after rotavirus) in children under 5 years old. 5 The consumption of antibiotics has saved the lives of a large number of patients with bacterial infections. However, it is dangerous that the global consumption of antibiotics and per capita consumption is growing rapidly.…”

Section: Introductionmentioning

confidence: 99%

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Monitoring Longitudinal Trends and Assessment of the Health Risk of Shigella flexneri Antimicrobial Resistance

Fang

Huang

et al. 2023

Environ. Sci. Technol.

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Shigella flexneri infection is the main cause of diarrhea in humans worldwide. The emergence of antimicrobial resistance (AMR) of S. flexneri is a growing public health threat worldwide, while large-scale studies monitoring the longitudinal AMR trends of isolates remain scarce. Here, the AMR gene (ARG) profiles of 717 S. flexneri isolates from 1920 to 2020 worldwide were determined. The results showed that the average number of ARGs in isolates has increased significantly, from 19.2 ± 2.4 before 1970 to 29.6 ± 5.3 after 2010. In addition, mobile genetic elements were important contributors to ARGs in S. flexneri isolates. The results of the structural equation model showed that the human development index drove the consumption of antibiotics and indirectly promoted the antibiotic resistance. Finally, a machine learning algorithm was used to predict the antibiotic resistance risk of global terrestrial S. flexneri isolates and successfully map the antibiotic resistance threats in global land habitats with over 80% accuracy. Collectively, this study monitored the longitudinal AMR trends, quantitatively surveilled the health risk of S. flexneri AMR, and provided a theoretical basis for mitigating the threat of antibiotic resistance.

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Section: Introductionmentioning

confidence: 99%

“…Shigellosis, the cause of bacillary dysentery caused by S. flexneri, is the second leading cause of diarrheal deaths (after rotavirus) in children under 5 years old …”

Section: Introductionmentioning

confidence: 99%

Monitoring Longitudinal Trends and Assessment of the Health Risk of Shigella flexneri Antimicrobial Resistance

Fang

Huang

et al. 2023

Environ. Sci. Technol.

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“…Thymoquinone, the main phenolic content of BCO, exhibits many biological activities, including anticancer (Samarghandian et al, 2019), antioxidant, phenolic (Yildiz et al, 2020), antimicrobial (Fan et al, 2021), and anti‐inflammatory activities (Taka et al, 2015). The thymoquinone content of cold‐pressed BCO was 299.12 ± 0.70 (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Structural characterization and oxidative stability of black cumin oil oleogels prepared with natural waxes

Orhan

Eroğlu

2022

Food Processing Preservation

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In this study, black cumin oil (BCO) oleogel was produced using natural waxes (beeswax: BW, sunflower wax: SFW, and carnauba wax: CW) to offer a healthy alternative to shortening. Oiling-off was observed only in the BW oleogel (3.88 ± 0.02%).The CW oleogel had the lowest E a (354.82 kJ/mol), indicating greater resistance to thermal degradation. The highest G′ and G″ were observed in the CW oleogel, while the lowest was observed in the BW oleogel. All oleogel samples displayed weak gel properties (tan δ > 0.1), but the lowest tan δ value belonged to the CW oleogel (0.12).After 4 weeks of storage, the peroxide values of the oleogels were lower than those of bulk oil (p < .05). Finally, the oxidation results showed that the BCO structuring by the waxes effectively protected it from oxidation over eight weeks of storage. Novelty impact statement:The most important innovation of the present study was to investigate the effect of BCO oleogelation on the stability of thymoquinone, the main phenolic compound of BCO. The BCO oleogels had higher thymoquinone content during the first half of the storage period compared with bulk BCO, showing that the oleogel network provides a protective effect against oxidation and thermal degradation.

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“…The ability of biofilm formation of A. baumannii depends on its outer membrane protein A (ompA) and pili, which mediate the attachment of the biofilm to the surface [9]. TQ has earlier been shown to inhibit the formation of the biofilm by Listeria monocytogenes, Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumoniae, Shigella flexneri, Candida albicans, and Candida glabrata [21][22][23][24][25]. In agreement with the earlier reports, the results of the present study demonstrated that TQ amazingly inhibited biofilm formation and moreover, it also substantially eradicated the preformed biofilm by A. baumannii.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial, Immunomodulatory and Anti-Inflammatory Potential of Liposomal Thymoquinone: Implications in the Treatment of Bacterial Pneumonia in Immunocompromised Mice

et al. 2021

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Acinetobacter baumannii has recently been increasing as an aggressive pathogen in immunocompromised persons. In the present study, we determined the in vitro antibacterial and anti-biofilm activity of thymoquinone (TQ) against A. baumannii. A liposomal formulation of TQ (Lip-TQ) was prepared and its therapeutic potential was investigated in the treatment of A. baumannii infection in immunocompromised mice. Leukopenia was induced in mice by injecting cyclophosphamide (CYP) at a dose of 200 mg/kg and the leukopenic mice were infected with 1 × 106 CFUs of A. baumannii. The effectiveness of free TQ or Lip-TQ against A. baumannii infection was assessed by analyzing the survival rate and bacterial burden. Moreover, the efficacy of Lip-TQ was also studied by examining the systemic inflammatory markers and the histological changes in the lung tissues. The results showed that the mice in the group treated with Lip-TQ at a dose of 10 mg/kg exhibited a 60% survival rate on day 40 post-infection, whereas all the mice treated with free TQ at the same dose died within this duration. Likewise, the lowest bacterial burden was found in the lung tissue of mice treated with Lip-TQ (10 mg/kg). Besides, Lip-TQ treatment remarkably alleviated the infection-associated inflammation, oxidative stress, and histological changes in the lung tissues. Based on the findings of the present study, we recommend considering Lip-TQ as a valuable therapeutic formulation in the treatment of A. baumannii-associated pneumonia in immunocompromised subjects.

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Antimicrobial and anti-biofilm activity of thymoquinone against Shigella flexneri

Cited by 17 publications

References 31 publications

Monitoring Longitudinal Trends and Assessment of the Health Risk of Shigella flexneri Antimicrobial Resistance

Monitoring Longitudinal Trends and Assessment of the Health Risk of Shigella flexneri Antimicrobial Resistance

Structural characterization and oxidative stability of black cumin oil oleogels prepared with natural waxes

Antimicrobial, Immunomodulatory and Anti-Inflammatory Potential of Liposomal Thymoquinone: Implications in the Treatment of Bacterial Pneumonia in Immunocompromised Mice

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