Antimicrobial and Anti-inflammatory Effects of a Novel Peptide From the Skin of Frog Microhyla pulchra

Tian, Maolin; Liu, Junfang; Chai, Jinwei; Wu, Jiena; Xu, Xueqing

doi:10.3389/fphar.2021.783108

Cited by 11 publications

(5 citation statements)

References 35 publications

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“…By comparing their sequences and physicochemical properties, Cath-MH is found to contain more positively charged residues than Brevinin-1GHd. Furtherly, Brevinin-1GHd showed more significant anti-inflammatory activity than Brevinin-2MP in the treatment of carrageenan-induced acute inflammation in animal models when comparing present results with data reported by Tian (Tian et al, 2021). Although Brevinin-2MP has one more cation (Arg) than Brevinin-1GHd, it also has two more negatively charged residuals (Asp + Glu).…”

Section: Discussionsupporting

confidence: 74%

“…Membrane binding assays were performed with Brevinin-1GHd as previously described with slight modification (Tian et al, 2021). Shortly, FITC-labeled Brevinin-1GHd was generated with FITC conjugation kit (Sangon Biotech, Shanghai, China) according to the manufacture's manual.…”

Section: Membrane Binding Assaymentioning

confidence: 99%

“…The in vivo anti-inflammatory effects of Brevinin-1GHd were evaluated with the carrageenan-induced mice as described previously (Tian et al, 2021). Shortly, at the beginning of the experiment, the paw volume up to the ankle was determined in all mice using a volume meter (Taimeng PV-2007500, China).…”

Section: Paw Edema Assaymentioning

confidence: 99%

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The first Brevinin-1 antimicrobial peptide with LPS-neutralizing and anti-inflammatory activities in vitro and in vivo

Tian

Wang²,

Liang³

et al. 2023

Front. Microbiol.

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Antimicrobial peptide is one important component of the first protective barrier of organisms. They not only have potent antimicrobial activity which can protect the body from the invading pathogens, but also participate in the immune regulation of the body. In this study, a Brevinin-1 peptide named by Brevinin-1GHd was identified from Hoplobatrachus rugulosus, and the similarity of mature peptide sequence among Brevinin-1GHd, Brevinin-1HL and Brevinin-1GHa supported the close species relationship between H. rugulosus, Hylarana latouchii and Hylarana guertheri. Moreover, the secondary structure of Brevinin-1GHd was found to possess α-helical characteristics and high thermal stability. In addition, Brevinin-1GHd could bind to LPS with a Kd value of 6.49 ± 5.40 mM and suppress the release of TNF-α, NO, IL-6 and IL-1β by inactivation of MAPK signaling pathway in RAW 264.7 cells induced by LPS. Furtherly, Brevinin-1GHd had a significant inhibitory effect on acute edema development in the right paw of mice injected by carrageenan. Thus, the significant LPS-neutralizing and anti-inflammatory activities of Brevinin-1GHd were demonstrated in this study, which made it become the first Brevinin-1 family peptide with anti-inflammatory activity reported so far, and the biological activity of Brevinin-1GHd made it promising to be a novel therapeutic drug for infectious inflammation.

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Section: Discussionsupporting

confidence: 74%

Section: Membrane Binding Assaymentioning

confidence: 99%

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The first Brevinin-1 antimicrobial peptide with LPS-neutralizing and anti-inflammatory activities in vitro and in vivo

Tian

Wang²,

Liang³

et al. 2023

Front. Microbiol.

Self Cite

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“…For instance, cathelicidin-PP and Nv-CATH are two cathelicidin-like AMPs isolated from Polypedates puerensis and Nanorana ventripunctata, respectively [74,75]. Brevinin-1FL and revinine-2MP belong to the revinine family of AMPs, which were identified from the skin of Fejervarya limnocharis and Microhyla pulchra [76,77]. Analogues of temporin-1Cea, temporin-1Ceb, and temporin-1Cec, namely LK2(6), L-K6, and 2K4L, were derived from the skin secretion of Rana chensinensis [78][79][80].…”

Section: Discussionmentioning

confidence: 99%

Chensinin-1b Alleviates DSS-Induced Inflammatory Bowel Disease by Inducing Macrophage Switching from the M1 to the M2 Phenotype

Sun,

Li,

Duan

et al. 2024

Biomedicines

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Inflammatory bowel disease (IBD) is a chronic relapsing inflammatory disorder with an increasing prevalence worldwide. Macrophage polarization is involved in the pathogenesis of IBD. Repolarization of macrophage has thus emerged as a novel therapeutic approach for managing IBD. Chensinin-1b, derived from the skin of Rana chensinensis, is a derivative of a native antimicrobial peptide (AMP). It shows anti-inflammatory effects in sepsis models and can potentially modulate macrophage polarization. The objective of this research was to study the role of chensinin-1b in macrophage polarization and dextran sulfate sodium (DSS)-induced colitis. RAW264.7 macrophages were polarized to the M1 phenotype using lipopolysaccharide (LPS) and simultaneously administered chensinin-1b at various concentrations. The ability of chenisnin-1b to reorient macrophage polarization was assessed by ELISA, qRT-PCR, and flow cytometry analysis. The addition of chensinin-1b significantly restrained the expression of M1-associated proinflammatory cytokines and surface markers, including TNF-α, IL-6, NO, and CD86, and exaggerated the expression of M2-associated anti-inflammatory cytokines and surface markers, including IL-10, TGF-β1, Arg-1, Fizz1, Chil3, and CD206. Mechanistically, via Western Blotting, we revealed that chensinin-1b induces macrophage polarization from the M1 to the M2 phenotype by inhibiting the phosphorylation of nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK). In mouse models of colitis, intraperitoneal administration of chensinin-1b alleviated symptoms induced by DSS, including weight loss, elevated disease activity index (DAI) scores, colon shortening, colonic tissue damage, and splenomegaly. Consistent with our in vitro data, chensinin-1b induced significant decreases in the expression of M1 phenotype biomarkers and increases in the expression of M2 phenotype biomarkers in the mouse colitis model. Furthermore, chensinin-1b treatment repressesed NF-κB phosphorylation in vivo. Overall, our data showed that chensinin-1b attenuates IBD by repolarizing macrophages from the M1 to the M2 phenotype, suggesting its potential as a therapeutic candidate for IBD.

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“…Humans have the capacity to produce a wide variety of AMPs that form the host’s first line of defense against infection and generally play an important role as effector factors of the innate immune system [ 5 , 6 , 7 , 8 , 42 , 43 ]. AMPs show strong activity (even in low concentrations in vitro) against many pathogenic microorganisms and have anti-inflammatory properties, making them important compounds (e.g., Granulysin-derived peptides, CEN1HC-Br, LL-37, MX-594AN) for the development of new therapeutic agents in infectious and inflammatory diseases [ 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 ]. However, it should be mentioned that the effects of AMPs on the immune system are extensive and complex, and in addition to their anti-inflammatory properties, many AMPs exhibit strong pro-inflammatory properties, reflecting the need to tightly control any immune response [ 54 , 55 ].…”

Section: Therapeutic Potential Of Ampsmentioning

confidence: 99%

S100 Proteins as Novel Therapeutic Targets in Psoriasis and Other Autoimmune Diseases

Kurpet

Chwatko

2022

Molecules

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Psoriasis is one of the most common inflammatory skin diseases affecting about 1–3% of the population. One of the characteristic abnormalities in psoriasis is the excessive production of antimicrobial peptides and proteins, which play an essential role in the pathogenesis of the disease. Antimicrobial peptides and proteins can be expressed differently in normal and diseased skin, reflecting their usefulness as diagnostic biomarkers. Moreover, due to their very important functions in innate immunity, members of host defense peptides and proteins are currently considered to be promising new therapeutic targets for many inflammatory diseases. Koebnerisin (S100A15) belongs to an S100 family of antimicrobial proteins, which constitute the multigenetic group of calcium-binding proteins involved in ion-dependent cellular functions and regulation of immune mechanisms. S100A15 was first discovered to be overexpressed in ‘koebnerized’ psoriatic skin, indicating its involvement in the disease phenotype and the same promising potential as a new therapeutic target. This review describes the involvement of antimicrobial peptides and proteins in inflammatory diseases’ development and therapy. The discussion focuses on S100 proteins, especially koebnerisin, which may be involved in the underlying mechanism of the Köebner phenomenon in psoriasis, as well as other immune-mediated inflammatory diseases described in the last decade.

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Antimicrobial and Anti-inflammatory Effects of a Novel Peptide From the Skin of Frog Microhyla pulchra

Cited by 11 publications

References 35 publications

The first Brevinin-1 antimicrobial peptide with LPS-neutralizing and anti-inflammatory activities in vitro and in vivo

The first Brevinin-1 antimicrobial peptide with LPS-neutralizing and anti-inflammatory activities in vitro and in vivo

Chensinin-1b Alleviates DSS-Induced Inflammatory Bowel Disease by Inducing Macrophage Switching from the M1 to the M2 Phenotype

S100 Proteins as Novel Therapeutic Targets in Psoriasis and Other Autoimmune Diseases

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