Antimicrobial and anti-inflammatory potential of Angelica dahurica and Rheum officinale extract accelerates wound healing in Staphylococcus aureus-infected wounds

Abstract

Wound infection is a serious clinical problem, and the most common infection-causing bacteria are Staphylococcus aureus and Pseudomonas aeruginosa. Angelica dahurica and Rheum officinale extract (ARE) was reported to accelerate excisional wound healing in rats. In this study, we investigated the therapeutic effects of ARE on bacterial-infected wounds. Thirty Sprague-Dawley rats were divided into three groups: normal saline (NS), ARE, and biomycin ointment (BO). Full-thickness dorsal excisions in all the rats were infected with 108 colony-forming units of S. aureus; the treatments were applied once daily for 7 days. Results showed that the residual wound area in ARE group was smaller than those in NS and BO groups. TBCs on wound sites gradually decreased in ARE and BO groups. The body temperature and plasma inflammatory cytokines (TNF-α, IL-6) levels increased after bacterial infection at 24 h in all groups. After treatment, BT and inflammatory cytokines levels decreased in ARE group. Histological observations showed ARE group exhibited earlier scab formation, denser dermal granulation tissue, thicker epidermis, and more angiogenesis markers than the other groups. In conclusion, ARE accelerated wound healing in S. aureus-infected wounds. We proposed ARE exhibited potential antimicrobial and anti-inflammatory effects and stimulated angiogenesis, thus improving healing in infected wounds.

Angelica Dahurica Regulated the Polarization of Macrophages and Accelerated Wound Healing in Diabetes: A Network Pharmacology Study and In Vivo Experimental Validation

“…Following A. dahurica treatment, the secretion of inflammatory factors, such as IL-1β, TNF-α, and IL-6 also reduced significantly, which is of great significance to diminish wound inflammatory cell infiltration. This finding is consistent with previous studies that indicated A. dahurica decreased inflammatory cytokines levels, such as TNF-α, IL-6, and accelerated wound closure in rats ( Yang et al, 2020 ). Furthermore, a recent study reported that A. dahurica decreased the mRNA expression of IL-6, IL-4, and TNF-α in the tissues of mice skin.…”

A Water-Soluble Antibiotic in Rhubarb Stalk Shows an Unusual Pattern of Multiple Zones of Inhibition and Preferentially Kills Slow-Growing Bacteria

“…Most published research on rhubarb has been conducted using organic extract of the roots. Organic extract of Rheum officinale was shown to have strong activity against S. aureus [ 33 ]. Emodin, an anthraquinone in the organic extract, can block the SARS coronavirus spike protein and angiotensin-converting enzyme 2 interaction [ 34 ].…”

Facile and Rapid Isolation of Oxypeucedanin Hydrate and Byakangelicin from Angelica dahurica by Using [Bmim]Tf2N Ionic Liquid

“…[ 1 , 2 , 3 , 4 ]. Several previous studies have also reported that the roots of A. dahurica exhibit various pharmacological functions, including antibacterial, anti-asthmatic, hypotensive, anti-inflammatory, antioxidation, anti-cancer, and anti-Alzheimer effects [ 5 , 6 , 7 , 8 , 9 , 10 , 11 ]. Pharmacological functions of A. dahurica are generally attributed to coumarins (oxypeucedanin, bergapten, imperatorin, cnidilin, isoimperatorin, xanthotoxol, oxypeucedanin hydrate, byakangelicin, etc.…”