Antimicrobial and Antiadhesion Activity of Biosurfactants from Rhodotorula glutinis Grown on n-dodecane

Csutak, Ortansa; Sârbu, Ionela; Rusu, Elena; Vassu, Tatiana; Corbu, Viorica Maria

doi:10.37358/rc.20.5.8117

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…In order to avoid evaporation of the volatile carbon source, the flask was covered with parafilm and then incubated at 28 °C and 150 rpm for 72 h. At the end of the incubation period, the culture was centrifuged for 10 min at 10,000 rpm and 4 °C, and the recovered supernatant was filtered using sterile Nalgene filter units with 0.2 µm size pores. The cell-free broth obtained after filtering the supernatant was further used as a crude biosurfactant [ 29 , 34 ].…”

Section: Methodsmentioning

confidence: 99%

Bioremediation of Wastewater Using Yeast Strains: An Assessment of Contaminant Removal Efficiency

Nicula

Lungulescu

Rimbu

et al. 2023

IJERPH

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The main goal of wastewater treatment is to significantly reduce organic compounds, micronutrients (nitrogen and phosphorus) and heavy metals and other contaminants (pathogens, pharmaceuticals and industrial chemicals). In this work, the efficiency of removing different contaminants (COD, NO3−, NO2−, NH4+, PO43−, SO42−, Pb2+, Cd2+) from synthetic wastewater was tested using five different yeast strains: Kluyveromyces marxianus CMGBP16 (P1), Saccharomyces cerevisiae S228C (P2), Saccharomyces cerevisiae CM6B70 (P3), Saccharomyces cerevisiae CMGB234 (P4) and Pichia anomala CMGB88 (P5). The results showed a removal efficiency of up to 70% of COD, 97% of nitrate, 80% of nitrite, 93% of phosphate and 70% of sulfate ions for synthetic wastewater contaminated with Pb2+ (43 mg/L) and Cd2+ ions (39 mg/L). In contrast, the results showed an increase in ammonium ions, especially in the presence of Pb2+ ions. The yeast strains showed a high capacity to reduce Pb2+ (up to 96%) and Cd2+ (up to 40%) ions compared to the initial concentrations. In presence of a crude biosurfactant, the removal efficiency increased up to 99% for Pb2+ and 56% for Cd2+ simultaneously with an increase in yeast biomass of up to 11 times. The results, which were obtained in the absence of aeration and in neutral pH conditions, proved a high potential for practical applications in the biotreatment of the wastewater and the recovery of Pb and Cd ions, with a high benefit–cost ratio.

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Section: Methodsmentioning

confidence: 99%

Bioremediation of Wastewater Using Yeast Strains: An Assessment of Contaminant Removal Efficiency

Nicula

Lungulescu

Rimbu

et al. 2023

IJERPH

View full text Add to dashboard Cite

show abstract

“…The biosurfactant was obtained using a protocol described previously by Nicula et al [31]. The cell-free broth obtained after filtering the supernatant was further used as a crude biosurfactant [32,33].…”

Section: Biosurfactant Effect Onmentioning

confidence: 99%

Yarrowia lipolytica CMGB32 Biosurfactants Produced Using n-Hexadecane: Developing Strategies for Environmental Remediation

Csutak,

Nicula,

Lungulescu

et al. 2024

Applied Sciences

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The yeast Yarrowia lipolytica degrades petroleum compounds, including alkanes, via the monoterminal oxidation pathway, the hydrophobic carbon substrate assimilation is mediated by biosurfactants, and extracellular amphiphilic molecules are produced by the yeast cell. This study focuses on the ability of the strain Y. lipolytica CMGB32 to degrade n-hexadecane by producing biosurfactants with high potential for bioremediation. The hydrocarbon-degrading potential of the yeast strain was observed via a 2,6-dichlorophenolindophenol (DCPIP) test in Bushnell–Hass medium with 1% n-hexadecane, and cell hydrophobicity was expressed as microbial adhesion to hydrocarbons (MATH). Biosurfactant production on yeast peptone (YP) with 1% n-hexadecane was estimated after 72 h using the emulsification index (E24%) against toluene. Crude biosurfactant (cell-free broth) stability tests were performed at different temperatures (4 °C, 70 °C) and NaCl concentrations (2–10%). The effects of a biosurfactant on synthetic wastewater remediation comprised the growth curves (OD measurements) of natural heavy metal degrader Rhodotorula mucilaginosa, determination of nutrients (spectrophotometrically), physico-chemical parameters, and removal capacity of lead and cadmium ions (via inductively coupled plasma mass spectrometry—ICP-MS). The antimicrobial and anti-adherence activities of 20 mg/mL and 40 mg/mL of the biosurfactant against pathogenic Candida krusei strains involved growth observations and the crystal violet microtiter method. The DCPIP decolorization occurred after six days, corresponding to the maximum growth phase of the Y. lipolytica culture. After 72 h, the cells presented high hydrophobicity (82.61% MATH) and stable biosurfactant production (E24% 47%). The crude biosurfactant (5%) increased the growth of R. mucilaginosa strains cultivated on synthetic wastewater cultures contaminated with Pb2+ and Cd2+, increased the conductivity and COD (86%) of the samples, and determined Pb2+ (66%) and Cd2+ (42%) ions reduction. The concentrated biosurfactant inhibited C. krusei growth (70%) and biofilm adherence. In conclusion, Y. lipolytica CMGB32 shows important potential for development of biosurfactant-based technologies for the remediation of heavy-metal- and emerging pathogen-contaminated wastewaters.

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Recent research advances on polysaccharide-, peptide-, and protein-based hemostatic materials: A review

Shao,

Wu,

Xiao

et al. 2024

International Journal of Biological Macromolecules

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Antimicrobial and Antiadhesion Activity of Biosurfactants from Rhodotorula glutinis Grown on n-dodecane

Cited by 4 publications

References 16 publications

Bioremediation of Wastewater Using Yeast Strains: An Assessment of Contaminant Removal Efficiency

Bioremediation of Wastewater Using Yeast Strains: An Assessment of Contaminant Removal Efficiency

Yarrowia lipolytica CMGB32 Biosurfactants Produced Using n-Hexadecane: Developing Strategies for Environmental Remediation

Recent research advances on polysaccharide-, peptide-, and protein-based hemostatic materials: A review

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