Antimicrobial and Antibiofilm Molecules Produced by Marine Bacteria

Desriac, Florie; Rodrigues, Sophie; Doghri, Ibtissem; Sablé, Sophie; Lanneluc, Isabelle; Fleury, Yannick; Bazire, Alexis; Dufour, Alain

doi:10.1002/9783527801718.ch25

Cited by 3 publications

(4 citation statements)

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“…IIIA004 marine bacterium, active against various bacteria. As described in several studies, marine bacteria are a potential source of effective anti-biofilm compounds [ 23 ]. Some of them were shown to secrete anti-biofilm molecules active against a wide range of Gram-positive and Gram-negative bacteria including Staphylococcus aureus , Listeria monocytogenes, and Salmonella typhimurium [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the Pseudoalteromonas genus is of great interest to the scientific community because of its prolific metabolite-producing ability [ 25 , 68 ]. Compounds of interest include toxic proteins, polyanionic exopolymers, substituted phenolic and pyrrole-containing alkaloids, cyclic peptides and a range of bromine-substituted compounds with antimicrobial, anti-fouling, algicidal, and various pharmaceutically relevant activities [ 23 , 25 ]. However, among the anti-biofilm proteinaceous compounds synthesized by Pseudoalteromonas strains, only the 190-kDa autotoxic protein (AlpP) produced by P. tunicata D2 and the 23-kDa alginate lyase (AlyP1400) produced by Pseudoalteromonas sp.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, this important field of investigation requires the development of ecofriendly anti-biofilm molecules [ 12 , 13 , 16 , 17 ]. Various studies have demonstrated that marine microbes are promising potential sources of bioactive compounds, including antibiofilm molecules, that act by regulating biofilm architecture, by inhibiting the attachment of microorganisms and thus the settlement of invertebrate larvae and macro-algal spores or by mediating the release of cells from biofilms during the dispersal stage of the biofilm life cycle [ 15 , 18 , 19 , 20 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Anti-Biofilm Activity of a Low Weight Proteinaceous Molecule from the Marine Bacterium Pseudoalteromonas sp. IIIA004 against Marine Bacteria and Human Pathogen Biofilms

et al. 2020

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Pseudoalteromonas bacteria are known as potential bioactive metabolite producers. Because of the need to obtain natural molecules inhibiting the bacterial biofilms, we investigated the biofilm inhibitory activity of the marine bacterium Pseudoalteromonas sp. IIIA004 against the pioneer surface colonizer Roseovarius sp. VA014. The anti-biofilm activity from the culture supernatant of Pseudoalteromonas sp. IIIA004 (SNIIIA004) was characterized in microtiter plates (static conditions/polystyrene surface) and in flow cell chambers (dynamic conditions/glass surface). The Pseudoalteromonas exoproducts exhibited an inhibition of Roseovarius sp. VA014 biofilm formation as well as a strong biofilm dispersion, without affecting the bacterial growth. Microbial adhesion to solvent assays showed that SNIIIA004 did not change the broad hydrophilic and acid character of the Roseovarius strain surface. Bioassay-guided purification using solid-phase extraction and C18 reverse-phase-high-performance liquid chromatography (RP-HPLC) was performed from SNIIIA004 to isolate the proteinaceous active compound against the biofilm formation. This new anti-biofilm low weight molecule (< 3kDa), named P004, presented a wide spectrum of action on various bacterial biofilms, with 71% of sensitive strains including marine bacteria and human pathogens. Pseudoalteromonas sp. IIIA004 is a promising source of natural anti-biofilm compounds that combine several activities.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anti-Biofilm Activity of a Low Weight Proteinaceous Molecule from the Marine Bacterium Pseudoalteromonas sp. IIIA004 against Marine Bacteria and Human Pathogen Biofilms

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“…Previous reports indicated that secondary metabolites from micro-organisms may constitute one of the most promising sources of environmentally friendly active molecules, such as antifouling compounds, and that bacteria are the organisms of choice to obtain natural biodegradable products for antifouling coatings [34–36]. Indeed, marine bacteria have been shown to produce antibiofilm compounds against Gram-positive and Gram-negative bacteria in their culture supernatants [37, 38]. In the present study, bacterial antibiofilm compounds were looked for, from a collection of 86 marine bacteria, in order to prevent or disrupt the biofilms formed by Flavobacterium sp.…”

Section: Discussionmentioning

confidence: 99%

Antibiofilm activity in the culture supernatant of a marine Pseudomonas sp. bacterium

et al. 2020

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In the marine environment, most solid surfaces are covered by microbial biofilms, mainly composed of bacteria and diatoms. The negative effects of biofilms on materials and equipment are numerous and pose a major problem for industry and human activities. Since marine microorganisms are an important source of bioactive metabolites, it is possible that they synthesize natural ecofriendly molecules that inhibit the adhesion of organisms. In this work, the antibiofilm potential of marine bacteria was investigated using Flavobacterium sp. II2003 as a target. This strain is potentially a pioneer strain of bacteria that was previously selected from marine biofilms for its strong biofilm-forming ability. The culture supernatants of 86 marine heterotrophic bacteria were tested for their ability to inhibit Flavobacterium sp. II2003 biofilm formation and the Pseudomonas sp. IV2006 strain was identified as producing a strong antibiofilm activity. The Pseudomonas sp. IV2006 culture supernatant (SN IV2006) inhibited Flavobacterium sp. II2003 adhesion without killing the bacteria or inhibiting its growth. Moreover, SN IV2006 had no effect on the Flavobacterium sp. II2003 cell surface hydrophilic/hydrophobic and general Lewis acid-base characteristics, but modified the surface properties of glass, making it on the whole more hydrophilic and more alkaline and significantly reducing bacterial cell adhesion. The glass-coating molecules produced by Pseudomonas sp. IV2006 were found to probably be polysaccharides, whereas the antibiofilm molecules contained in SN IV2006 and acting during the 2 h adhesion step on glass and polystyrene surfaces would be proteinaceous. Finally, SN IV2006 exhibited a broad spectrum of antibiofilm activity on other marine bacteria such as Flavobacterium species that are pathogenic for fish, and human pathogens in both the medical environment, such as Staphylococcus aureus and Pseudomonas aeruginosa, and in the food industry, such as Yersinia enterocolitica. Thus, a wide range of applications could be envisaged for the SN IV2006 compounds, both in aquaculture and human health.

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Alterocin, an Antibiofilm Protein Secreted by Pseudoalteromonas sp. Strain 3J6

Jouault

Gobet

Simon

et al. 2020

Appl Environ Microbiol

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The aim was to identify and study the antibiofilm protein secreted by the marine bacterium Pseudoalteromonas sp. 3J6. The latter is active against marine and terrestrial bacteria, including Pseudomonas aeruginosa clinical strains forming different biofilm types. Several amino acid sequences were obtained from the partially purified antibiofilm protein, named alterocin. The Pseudoalteromonas sp. 3J6 genome was sequenced and a candidate alt gene was identified by comparing the genome-encoded proteins to the sequences from purified alterocin. Expressing the alt gene in another non-active Pseudoalteromonas sp. strain, 3J3, demonstrated that it is responsible for the antibiofilm activity. Alterocin is a 139-residue protein including a predicted 20-residue signal sequence, which would be cleaved off upon export by the general secretion system. No sequence homology was found between alterocin and proteins of known functions. The alt gene is not part of an operon and adjacent genes do not seem related to alterocin production, immunity or regulation, suggesting that these functions are not fulfilled by devoted proteins. During liquid growth, the alt mRNA level peaked during the stationary phase. A single promoter was experimentally identified and several inverted repeats could be binding sites for regulators. alt genes were found in about 30% of the Pseudoalteromonas genomes, and in only few instances of other marine bacteria of the Hahella and Paraglaciecola genera. Comparative genomics yielded the hypotheses that alt gene losses occurred within the Pseudoalteromonas genus. Overall, alterocin is a novel kind of antibiofilm protein of ecological and biotechnological interest. Importance Biofilms are microbial communities that develop on solid surfaces or interfaces, and are detrimental in a number of fields including for example food industry, aquaculture, and medicine. In the latter, antibiotics are insufficient to clear biofilm infections, leading to chronic infections such as in the case of infection by Pseudomonas aeruginosa of the lungs of cystic fibrosis patients. Antibiofilm molecules are thus urgently needed to be used in conjunction with conventional antibiotics, as well as in other fields of application, especially if they are environmentally friendly molecules. Here, we describe alterocin, a novel antibiofilm protein secreted by a marine bacterium belonging to the Pseudoalteromonas genus, and its gene. Alterocin homologs were found in about 30% of Pseudoalteromonas strains, indicating that this new family of antibiofilm proteins likely plays an important albeit non-essential function in the biology of these bacteria. This work opens up the possibility of a variety of applications.

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Antimicrobial and Antibiofilm Molecules Produced by Marine Bacteria

Cited by 3 publications

References 130 publications

Anti-Biofilm Activity of a Low Weight Proteinaceous Molecule from the Marine Bacterium Pseudoalteromonas sp. IIIA004 against Marine Bacteria and Human Pathogen Biofilms

Anti-Biofilm Activity of a Low Weight Proteinaceous Molecule from the Marine Bacterium Pseudoalteromonas sp. IIIA004 against Marine Bacteria and Human Pathogen Biofilms

Antibiofilm activity in the culture supernatant of a marine Pseudomonas sp. bacterium

Alterocin, an Antibiofilm Protein Secreted by Pseudoalteromonas sp. Strain 3J6

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