Antimicrobial and antioxidant activities of different solvent extracts from fermented and green honeybush (Cyclopia intermedia) plant material

Dube, Phumuzile; Meyer, Samantha; Marnewick, Jeanine L.

doi:10.1016/j.sajb.2016.10.010

Cited by 38 publications

(33 citation statements)

References 45 publications

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“…Lower extract concentrations (0.1-1 ng/ml) were also more effective. Overall, our data are in line with existing literature reporting an antioxidant effect of honeybush [ 19 – 22 ]. However, the effects of honeybush extracts on neuronal cells and specifically on mitochondrial function have been investigated here for the first time.…”

Section: Discussionsupporting

confidence: 92%

“…Increased consumption and popularity of honeybush came along with increasing research interest in order to reveal new bioactivities and to examine its potential use as a nutraceutical and functional food [ 16 , 18 ]. Quite predictably due to their phenolic composition, honeybush extracts have been shown to possess antioxidant activities which are of great importance and interest in the research of oxidative stress-related diseases [ 19 – 22 ]. Considering on the one hand the evidence of its antioxidant capacity and on the other hand the need for mitochondria-targeting antioxidant substances for use in the prevention of oxidative damage or the amelioration of increased oxidative stress levels, we hypothesized that honeybush could possess some beneficial mitochondria-enhancing properties.…”

Section: Introductionmentioning

confidence: 99%

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Honeybush Extracts (Cyclopia spp.) Rescue Mitochondrial Functions and Bioenergetics against Oxidative Injury

Agapouda

Butterweck

Hamburger

et al. 2020

Oxidative Medicine and Cellular Longevity

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Mitochondrial dysfunction plays a major role not only in the pathogenesis of many oxidative stress or age-related diseases such as neurodegenerative as well as mental disorders but also in normal aging. There is evidence that oxidative stress and mitochondrial dysfunction are the most upstream and common events in the pathomechanisms of neurodegeneration. Cyclopia species are endemic South African plants and some have a long tradition of use as herbal tea, known as honeybush tea. Extracts of the tea are gaining more scientific attention due to their phenolic composition. In the present study, we tested not only the in vitro mitochondria-enhancing properties of honeybush extracts under physiological conditions but also their ameliorative properties under oxidative stress situations. Hot water and ethanolic extracts of C. subternata, C. genistoides, and C. longifolia were investigated. Pretreatment of human neuroblastoma SH-SY5Y cells with honeybush extracts, at a concentration range of 0.1-1 ng/ml, had a beneficial effect on bioenergetics as it increased ATP production, respiration, and mitochondrial membrane potential (MMP) after 24 hours under physiological conditions. The aqueous extracts of C. subternata and C. genistoides, in particular, showed a protective effect by rescuing the bioenergetic and mitochondrial deficits under oxidative stress conditions (400 μM H2O2 for 3 hours). These findings indicate that honeybush extracts could constitute candidates for the prevention of oxidative stress with an impact on aging processes and age-related neurodegenerative disorders potentially leading to the development of a condition-specific nutraceutical.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Honeybush Extracts (Cyclopia spp.) Rescue Mitochondrial Functions and Bioenergetics against Oxidative Injury

Agapouda

Butterweck

Hamburger

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…Compared with other plant species, the contents of total flavonoid and total polyphenol in the C. paliurus leaves were much higher than those of aqueous extracts from the leaves of indigo plant and Tossa jute, but similar to that of ginkgo leaves (Sati et al, 2013; Heo et al, 2014; Yakoub et al, 2018). However, the total polyphenol content in C. paliurus leaves was lower than those of aqueous extracts from Cyclopia intermedia , ugni molinae and Nepeta species leaves (Dube, Meyer & Marnewick, 2017; López de Dicastillo et al, 2017; Dienaitė et al, 2018). Moreover, water-soluble polysaccharide content in the C. paliurus leaves was far beyond that of a previous study (Liu et al, 2018b), which may be due to the difference in the extraction method used.…”

Section: Discussionmentioning

confidence: 73%

Phytochemical content and antioxidant activity in aqueous extracts of Cyclocarya paliurus leaves collected from different populations

Zhou

Lin

Fang

et al. 2019

PeerJ

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Cyclocarya paliurus (Batal) Iljinskaja is a multiple function tree species, but its main utilization is for the harvesting of its leaves as materials for tea production and recently as ingredients for the food industry. In this study aqueous extracts of C. paliurus leaves collected from 21 natural populations were evaluated for their phytochemical content and antioxidant activity. The content of water-soluble polysaccharide, total flavonoid and total polyphenol varied from 66.05 to 153.32 mg/g, 9.01 to 19.65 mg/g and 20.80 to 52.69 mg/g, respectively. Quercetin-3-O-glucuronide, aemferol-3-O-glucuronide and 3-O-caffeoylquinic acid were the major phenolic components in aqueous extracts of C. paliurus leaves. Both redundancy analysis and Pearson's correlation analysis indicated that water-soluble polysaccharide, total polyphenol, total flavonoid, quercetin-3-O-glucuronide, 3-O-caffeoylquinic acid and 4-O-caffeoylquinic acid were significantly correlated with antioxidant activity, but total polyphenol showed the greatest contribution to antioxidant capacity. The antioxidant activity of the 21 populations was classified into six distinct groups based on the squared Euclidean distance. These results would provide a theoretical basis for obtaining the greatest yield of targeted antioxidant phytochemicals of C. paliurus leaves for tea and food ingredient production.

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“…On the other hand, the decrease in sperm performance observed at 120 h of semen storage (CTR group) could be related to the proliferation of some contaminant bacterial genera, which appear to thrive in extended boar semen [31], given their resistance to the most commonly used antibiotics [32,33]. It has recently been reported that honeybush aqueous extract exerts antimicrobial activities [3] against some of the microorganisms (e.g., Staphylococcus spp., Streptococcus spp., and Candida spp.) commonly found in boar semen, which decrease sperm quality, thus affecting, for instance, the litter size [34,35].…”

Section: Discussionmentioning

confidence: 99%

“…Nowadays, special attention is paid to the use of honeybush as a phytotherapeutic agent, and about half of the related literature has been published in the last five years. Thus, for instance, the antioxidant, antimicrobial, and antimutagenic activities of honeybush have been tested in different cell types, with promising results [3,4]. However, the effects of honeybush on the reproductive function are still unknown, both in vivo and in vitro.…”

Section: Introductionmentioning

confidence: 99%

Pharmacological Activity of Honeybush (Cyclopia intermedia) in Boar Spermatozoa during Semen Storage and under Oxidative Stress

Ros-Santaella

Kadlec

Pintus

2020

Animals

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In recent decades, an increasing number of ethnopharmacological studies have been dedicated to medicinal plants from South African fynbos. Among these plants, honeybush (Cyclopia spp.) has become a popular tea, mainly due to its healthy properties and caffeine-free status. The antioxidant, antimutagenic, and antimicrobial properties of this plant have been reported in several cell types, but its effects on reproductive function are still unknown. Here, we assessed the effects of honeybush (Cyclopia intermedia) on boar sperm parameters under induced oxidative stress (Fe2+/ascorbate) and during five days of semen storage at 17 °C without oxidative stress. In both experiments, four concentrations (200, 50, 12.5, and 3.125 µg/mL) of fermented honeybush were tested. Our results show that honeybush enhances sperm parameters, and no toxic effects were observed at any of the tested extract concentrations. Interestingly, honeybush (12.5 µg/mL) improved the sperm motility and kinetic parameters, preserved the plasma membrane integrity, and reduced the lipid peroxidation in the samples exposed to Fe2+/ascorbate (p < 0.05). In the stored samples, positive effects of honeybush on sperm parameters (motility, kinetics, acrosome, and mitochondria) were observed from 48 h until 120 h of semen storage (p < 0.05). Our results clearly show the protective effects of honeybush on sperm samples, thus promoting its use as a natural source of antioxidants for boar semen.

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Antimicrobial and antioxidant activities of different solvent extracts from fermented and green honeybush (Cyclopia intermedia) plant material

Cited by 38 publications

References 45 publications

Honeybush Extracts (Cyclopia spp.) Rescue Mitochondrial Functions and Bioenergetics against Oxidative Injury

Honeybush Extracts (Cyclopia spp.) Rescue Mitochondrial Functions and Bioenergetics against Oxidative Injury

Phytochemical content and antioxidant activity in aqueous extracts of Cyclocarya paliurus leaves collected from different populations

Pharmacological Activity of Honeybush (Cyclopia intermedia) in Boar Spermatozoa during Semen Storage and under Oxidative Stress

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