ANTIMICROBIAL AND ANTIOXIDANT COMPOUNDS FROM THE INFUSION AND METHANOLIC EXTRACT OF Baccharis incarum (WEDD.) PERKINS

Abstract

In the traditional medicine of the South American highlands, the infusion and decoction of aerial parts of "tola", Baccharis incarum (Wedd.) Perkins (Asteraceae) is orally taken as an antiseptic, antipyretic and digestive or externally applied to relieve pain and inflammation.A plant infusion and methanolic (MeOH) extract of the aerial parts were compared for main constituents by HPLC-MS as well as assessed for antimicrobial and free radical scavenging activities. Assay-guided fractionation of the polar extracts was carried out using the ABTS•+ autographic as well as methicillin-resistant Staphylococcus aureus and Enterococcus faecalis bioautographic test. Seven compounds were isolated from the polar extracts and identified as chlorogenic acid, 3',4',5,7-tetrahydroxyflavone; dicaffeoyl quinic acid; 3',4',5,7-tetrahydroxy-3,6-dimethoxyflavone; 3',4 ',5,7-tetrahydroxy-3,6,8-trimethoxy flavone, 4',5,7-trihydroxy-3',3,6,8-tetramethoxyflavone and 4',5-dihydroxy-3',3,6,7,8-pentamethoxyflavone. The main constituents from the methanol extract and infusion were caffeic acid derivatives. This is the first report on the constituents of B. incarum infusion and show clear differences with previous phytochemical studies on the same plant. All isolated compounds showed antioxidant activity with SC 50 values of 1 to 10 µg/ml. The isolated flavones were active against methicillin-resistant Staphylococcus aureus and Enterococcus faecalis with MICs in the range 100 and >200 µg/ml.

Comparative study of antioxidant and anti-inflammatory activities and genotoxicity of alcoholic and aqueous extracts of four Fabiana species that grow in mountainous area of Argentina

“…The antioxidant activity found in Fabiana species was similar to the obtained activity of other shrubs species that grow in mountainous area of Argentina such as Baccharis incarum (Zampini et al, 2009b), Chuquiraga atacamensis, Parastrephia lucida (Zampini et al, 2008) and Zuccagnia punctata (Morán Vieyra et al, 2009). These plants are characterized by a high physiological plasticity not only for their salt tolerance limits, but also for the UV-radiations.…”

Antioxidant/Antibacterial Activities of a Topical Phytopharmaceutical Formulation Containing a Standardized Extract of Baccharis incarum, an Extremophile Plant Species from Argentine Puna

“…Figures A, B show the chromatograms of the tincture and the profiles of receptor solution of formulation, respectively. Previous phytochemical studies on the aerial parts of the polar extract of B. incarum described the isolation of chlorogenic acid and flavones (Zampini et al ., ). Chlorogenic acid and 4′,5‐dihydroxy‐3′,3,6,7,8‐pentamethoxyflavone were also identified in the receptor solution of formulation.…”

“…The results were expressed as gallic acid equivalent (GAE) per mL and quercetin equivalent (QE) per mL, respectively. The HPLC fingerprint was also performed to standardize the extract (Zampini et al ., ).…”

“…Previous phytochemical studies of the water infusion and water‐soluble portion of the methanol extract of B. incarum described the isolation of chlorogenic acid and dicaffeoylquinic acids as well as flavones (Zampini et al ., ). Antiinflammatory, antioxidant, antixanthine oxidase activities and antibacterial activity on antibiotic resistant Gram‐positive and Gram‐negative bacteria were demonstrated in B. incarum ethanol extract using 80° ethanol (Zampini et al ., ; Zampini, ; Alberto et al ., ).…”

Potentiality of standardized extract and isolated flavonoids from Zuccagnia punctata for the treatment of respiratory infections by Streptococcus pneumoniae: In vitro and in vivo studies

“…Consequently, alternative therapies are necessary. Recently, there has been considerable interest in the use of plant extracts as multi-drug complex as an alternative method to control pathogenic microorganisms (Zampini et al, 2005(Zampini et al, , 2007(Zampini et al, , 2009aWagner and Ulrich-Merzenich, 2009), and many compounds of plant products have been shown to be specifically targeted against resistant pathogenic bacteria (Zampini et al, 2005(Zampini et al, , 2009b.…”