Antimicrobial and Antioxidant Properties of Natural Postbiotics Derived from Five Lactic Acid Bacteria

Abstract

Background: The application of natural antimicrobial and antioxidant agents in food and pharmaceutical products has recently become a trend due to the high demand for them from consumers. Postbiotics are bioactive compounds that are produced when the healthy bacteria in the gut ferment fiber. Objectives: This study aimed to compare the antibacterial and antioxidant properties of postbiotics from 5 different lactic acid bacteria (LAB) including Lactiplantibacillus fermentum, Lactiplantibacillus plantarum, Lactiplantibacillus rhamnosus, Lactobacillus casei, and Lactobacillus acidophilus. Methods: Two different methods were adopted to obtain postbiotics (M1 and M2). M1 was the simple method in which the centrifugation was employed while in M2 method, ethyl acetate was used to obtain postbiotics. Agar disc diffusion, minimum inhibition concentration, and minimum bactericidal concentration were used to assess the antimicrobial activity of postbiotics. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) test was performed in order for investigating the antioxidant property. Results: The best results were recorded for L. casei compared to other LABs. Highest values of the agar disc diffusion method were obtained for L. casei. The inhibition zones for Salmonella enterica, Listeria monocytogenes, and Staphylococcus aureus, for examples, were 22 mm, 20 mm, and 19 mm, respectively. The postbiotic of L. casei also exhibited the most potent antioxidant activity among other probiotic bacteria. The data showed that M2 was a more effective method than the other method for acquiring postbiotics. Conclusions: It was recommended LABs postbiotics should be applied as antioxidant, antimicrobial, and preservatives in food and pharmaceutical industries due to their desired effects and natural characteristics.

Properties of postbiotics produced by probiotics: antimicrobial, antioxidant activities and production of vitamins, organic acids

“…Probiotic bacteria produce antimicrobial compounds such as organic acids and bacteriocins [26]. Contrary to our results, Noori et al, [13] found that L. acidophilus and L. plantarum were unable to eliminate the pathogenic bacteria; however, L. casei and L. rhamnosus postbiotics exhibited excellent antimicrobial activity. Magnusson et al, [27] reported that the antimicrobial activity L. plantarum depends on bacteriocins, organic acids and other inhibitory substances.…”

“…Moradi et al [12] mentioned that postbiotics are more resistant to food processing and they can remain active for longer periods. Noori et al [13] found that massive amounts of postbiotics were applied in the food industry to produce functional foods. In general, postbiotics have shown all the bene cial effects of probiotics [14,15].…”

Evaluation of Antioxidant and Antibacterial Effects of Lyophilized Cell-Free Probiotic Supernatants of Three Lactobacillus spp. and Their Cytocompatibility Against Periodontal Ligament Stem Cells

“…The role of different periodontopathogens, such as A. actinomycetemcomitans , in chronic and progressive periodontitis has been proven in previous studies ( 16 , 17 ). Due to the protective role of probiotics ( 18 , 19 ), the current study aimed to mark a novel investigation by comparatively examining both the antioxidant activity and the antimicrobial effect of mentioned LCFSs (i.e., L. acidophilus , L. casei , and L. paracasei ) against the oral bacteria (i.e., A. actinomycetemcomitans and S. mutans ) and the effects on the cell proliferation and migration of PDLSCs.…”

Microbiota-Derived Natural Products Targeting Cancer Stem Cells: Inside the Gut Pharma Factory

“…In this connection, cell-free supernatant (CFS), a solution that contains metabolites produced as a result of microbial growth, represents an invaluable metabolite-rich source. For instance, the antioxidant, antimicrobial, and anticancer properties of CFS have been demonstrated [142][143][144]. In 2016, An and Ha showed that the expression of particular CSC markers, CD44, CD133, CD166, and ALDH1, can be inhibited by Lactobacillus plantarum (LP) supernatant.…”