Antimicrobial and Antiviral Activities of Durable Cotton Fabrics Treated with Nanocomposite Based on Zinc Oxide Nanoparticles, Acyclovir, Nanochitosan, and Clove Oil

Ali

Reyad

et al. 2022

JoF

Self Cite

Mucormycosis is considered one of the most dangerous invasive fungal diseases. In this study, a facile, green and eco-friendly method was used to biosynthesize silver nanoparticles (AgNPs) using Pseudomonas indica S. Azhar, to combat fungi causing mucormycosis. The biosynthesis of AgNPs was validated by a progressive shift in the color of P. indica filtrate from colorless to brown, as well as the identification of a distinctive absorption peak at 420 nm using UV-vis spectroscopy. Fourier-transform infrared spectroscopy (FTIR) results indicated the existence of bioactive chemicals that are responsible for AgNP production. AgNPs with particle sizes ranging from 2.4 to 53.5 nm were discovered using transmission electron microscopy (TEM). Pattern peaks corresponding to the 111, 200, 220, 311, and 222 planes, which corresponded to face-centered cubic forms of metallic silver, were also discovered using X-ray diffraction (XRD). Moreover, antifungal activity measurements of biosynthesized AgNPs against Rhizopus Microsporus, Mucor racemosus, and Syncephalastrum racemosum were carried out. Results of antifungal activity analysis revealed that the biosynthesized AgNPs exhibited outstanding antifungal activity against all tested fungi at a concentration of 400 µg/mL, where minimum inhibitory concentrations (MIC) were 50, 50, and 100 µg/mL toward R. microsporus, S. racemosum, and M. racemosus respectively. In addition, the biosynthesized AgNPs revealed antioxidant activity, where IC50 was 31 µg/mL when compared to ascorbic acid (0.79 µg/mL). Furthermore, the biosynthesized AgNPs showed no cytotoxicity on the Vero normal cell line. In conclusion, the biosynthesized AgNPs in this study can be used as effective antifungals with safe use, particularly for fungi causing mucormycosis.

Section: Introductionmentioning

confidence: 99%

Pseudomonas indica-Mediated Silver Nanoparticles: Antifungal and Antioxidant Biogenic Tool for Suppressing Mucormycosis Fungi

Ali

Reyad

et al. 2022

JoF

Self Cite

“…Therefore, it is necessary to design and develop new compounds that overcome these limitations. Recently, nanoparticles (NPs) have been successfully used to deliver therapeutic agents [ 1 , 2 , 3 , 4 ] in the diagnosis of chronic diseases, to reduce bacterial infections, and in the food and clothing industries as antimicrobial agents [ 5 , 6 , 7 , 8 , 9 ]. Because of their antimicrobial properties and unique mode of action, specialized NPs provide an attractive alternative to conventional antibiotics in the development of a new generation of antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional Silver Nanoparticles Based on Chitosan: Antibacterial, Antibiofilm, Antifungal, Antioxidant, and Wound-Healing Activities

Shehabeldine

Ali

et al. 2022

JoF

Self Cite

The purpose of this study is to create chitosan-stabilized silver nanoparticles (Chi/Ag-NPs) and determine whether they were cytotoxic and also to determine their characteristic antibacterial, antibiofilm, and wound healing activities. Recently, the development of an efficient and environmentally friendly method for synthesizing metal nanoparticles based on polysaccharides has attracted a lot of interest in the field of nanotechnology. Colloidal Chi/Ag-NPs are prepared by chemical reduction of silver ions in the presence of Chi, giving Chi/Ag-NPs. Physiochemical properties are determined by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), and scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDX) analyses. TEM pictures indicate that the generated Chi/Ag-NPs are nearly spherical in shape with a thin chitosan covering around the Ag core and had sizes in the range of 9–65 nm. In vitro antibacterial activity was evaluated against Staphylococcus aureus and Pseudomonas aeruginosa by a resazurin-mediated microtiter plate assay. The highest activity was observed with the lowest concentration of Chi/Ag-NPs, which was 12.5 µg/mL for both bacterial strains. Additionally, Chi/Ag-NPs showed promising antifungal features against Candida albicans, Aspergillus fumigatus, Aspergillus terreus, and Aspergillus niger, where inhibition zones were 22, 29, 20, and 17 mm, respectively. Likewise, Chi/Ag-NPs revealed potential antioxidant activity is 92, 90, and 75% at concentrations of 4000, 2000, and 1000 µg/mL, where the IC50 of Chi/Ag-NPs was 261 µg/mL. Wound healing results illustrated that fibroblasts advanced toward the opening to close the scratch wound by roughly 50.5% after a 24-h exposure to Chi/Ag-NPs, greatly accelerating the wound healing process. In conclusion, a nanocomposite based on AgNPs and chitosan was successfully prepared and exhibited antibacterial, antibiofilm, antifungal, antioxidant, and wound healing activities that can be used in the medical field.

“…[1,2] Nanotechnology is an interdisciplinary field that encompasses a wide range of scientific and technological disciplines, including biomedical, pharmaceutical, environmental, agricultural, advanced structures, chemical sciences, physics, electronics, cotton fabrics, and information technology. [3][4][5][6][7][8][9][10][11][12][13] An emerging research field based on investigating the special characteristics such as size, shape, and distribution of NPs using nanotechnology procedures has been developed recently. [14][15][16][17][18][19][20][21][22][23] Inorganic nanoparticles, whose geometries exhibit fundamentally innovative and improved mechanical, chemical, and biological properties and functioning due to their nanoscale size, have sparked a lot of attention during the last few decades.…”

Section: Introductionmentioning

confidence: 99%

Green and ecofriendly biosynthesis of selenium nanoparticles using Urtica dioica (stinging nettle) leaf extract: Antimicrobial and anticancer activity

Hashem

2021

Biotechnology Journal

Self Cite

111

Background/Goal/Aim: Plant extract is affordable and does not require any particular conditions; rapid production of nanoparticles using plants offers more advantages than other approaches. Selenium nanoparticles (SeNPs) have received much attention in the last decade due to SeNPs diverse and different applications. Herein, this study aimed to biosynthesize SeNPs using aqueous extract of Urtica dioica leaf through green and ecofriendly method. Moreover to fully characterize SeNPs using different techniques, and to evaluate it for antimicrobial activity as well as anticancer activity.Main Methods and Major Results: SeNPs were biosynthesis using aqueous leaf extract of U. dioica (stinging nettle). The biosynthesized SeNPs were characterized using UV-visible spectroscopy (UV-Vis), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive electron spectroscopy (EDX), transmission electron microscopy (TEM), and thermalgravimetric analysis (TGA). Antimicrobial and anticancer activities of biosynthesized SeNPs were assessed. Results illustrated that SeNPs exhibited promising antibacterial activity against Gram-positive and Gram-negative bacteria, as well as unicellular and multi-cellular fungi. Moreover, minimal-inhibitory concentration (MIC) of