Antimicrobial, antibiofilm and cytotoxic activities of Hakea sericea Schrader extracts

Luís, Ângelo; Breitenfeld, Luiza; Ferreira, Susana; Duarte, Ana Paula; Domingues, Fernanda

doi:10.4103/0973-1296.127331

Cited by 19 publications

(3 citation statements)

References 17 publications

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“…After this time, the inhibition zones were checked, and the diameters were measured using a digital pachymeter. All experiments were carried out in triplicate [ 90 ].…”

Section: Methodsmentioning

confidence: 99%

Ayahuasca Beverages: Phytochemical Analysis and Biological Properties

et al. 2020

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Ayahuasca is a psychoactive beverage, originally consumed by indigenous Amazon tribes, of which consumption has been increasing worldwide. The aim of this study was to evaluate the phytochemical profile, as well as the antioxidant, anti-inflammatory and antimicrobial properties of decoctions of four individual plants, a commercial mixture and four mixtures of two individual plants used in the Ayahuasca preparation. For this purpose, a phytochemical characterization was performed, determining the content of flavonoids, total phenolic compounds, and analyzing the phenolic profile. Besides, 48 secondary metabolites were investigated by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q/TOF-MS) and their concentration estimated with real standards when present. The antioxidant activity was evaluated by both the β-carotene bleaching test and DPPH free radical scavenging assay, and the anti-inflammatory activity was determined by a protein denaturation method. Finally, the antimicrobial properties were evaluated using the disc diffusion assay, resazurin microtiter method, anti-quorum sensing and anti-biofilm activity assays. The obtained results showed that, in general, the samples have a high content of phenolic compounds and flavonoids with noticeable differences, reflecting on remarkable antioxidant and anti-inflammatory activities. Significant antimicrobial properties were also observed, with emphasis on the effect of B. caapi and P. harmala on planktonic and biofilm cells of A. baumannii, inhibiting both the biofilm formation and the production of violacein pigment.

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“…After this time, the inhibition zones were checked, and the diameters were measured using a digital pachymeter. All experiments were carried out in triplicate [ 90 ].…”

Section: Methodsmentioning

confidence: 99%

Ayahuasca Beverages: Phytochemical Analysis and Biological Properties

et al. 2020

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“…After incubation, all plates were checked for inhibition zones and the diameters were measured in millimeters. All experiments were carried out in triplicate [41].…”

Section: Methodsmentioning

confidence: 99%

Stumps of Eucalyptus globulus as a Source of Antioxidant and Antimicrobial Polyphenols

et al. 2014

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These past years have seen an enormous development of the area of natural antioxidants and antimicrobials. Eucalyptus globulus is widely cultivated in subtropical and Mediterranean regions in intensive short rotation coppice plantations. In the Portuguese context, E. globulus is the third species in terms of forest area. The stump is the basal part of the tree, including the near-the-ground stem portion and the woody roots that remain after stem felling. The purpose of this work was to study the phytochemical profile and to evaluate the antioxidant and antimicrobial properties of several crude stump wood and stump bark extracts of E. globulus, comparing it with similar extracts of E. globulus wood (industrial chips). The results showed the presence of high concentrations of total phenolic compounds (>200 mg GAE/g extract) and flavonoids (>10 mg QE/g extract) in E. globulus stump extracts. Generally the stump wood extracts stands out from the other ones, presenting the highest percentages of inhibition of linoleic acid oxidation. It was also possible to conclude that the extracts were more active against Gram-positive bacteria, presenting low MIC values. This study thus provides information supporting the economic valorization of E. globulus stump wood.

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“…Papa et al (2015) found that some bioactive compounds, which derived from marine cold-adapted bacteria, showed anti-biofilm activity on Staphylococci and P. aeruginosa. In another research (Luis et al, 2014), Hakea sericea Schrader extracts displayed an obvious effect by inhibiting the biofilm formation of S. aureus including MRSA. The study of artemisinin (qinghaosu) by 2015 Nobel Prize In this study, the antibacterial and anti-biofilm properties of the lipid extract of M. ootheca against P. aeruginosa were evaluated and compared with ciprofloxacin.…”

Section: Discussionmentioning

confidence: 92%

Antibacterial and anti-biofilm activity of the lipid extract from Mantidis ootheca on Pseudomonas aeruginosa

Wang

Zhang

Chanda

et al. 2018

J. Zhejiang Univ. Sci. B

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The aim of this study is to assess the antibacterial and anti-biofilm properties of the lipid extract from Mantidis ootheca against the gentamycin resistant Pseudomonas aeruginosa. The chemical composition of the lipid extract and its relative proportion were determined using the technique of gas chromatography coupled with mass spectrometry (GC-MS). Antibacterial susceptibility tests were performed using a disc diffusion assay and the minimum inhibition concentration (MIC) was determined by way of the agar dilution method. The anti-biofilm test was carried out with crystal violet staining and scanning electron microscopy (SEM). There were 16 compounds detected, and the most abundant components were sesquiterpenoids, monoterpenes, and trace aromatic compounds. The MIC for P. aeruginosa was 4 mg/ml and the eradication effect on preformed biofilms was established and compared with a ciprofloxacin control. The results of our study indicated that a lipid extract from M. ootheca could be used as a topical and antibacterial agent with anti-biofilm activity in the future.

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Antimicrobial, antibiofilm and cytotoxic activities of Hakea sericea Schrader extracts

Cited by 19 publications

References 17 publications

Ayahuasca Beverages: Phytochemical Analysis and Biological Properties

Ayahuasca Beverages: Phytochemical Analysis and Biological Properties

Stumps of Eucalyptus globulus as a Source of Antioxidant and Antimicrobial Polyphenols

Antibacterial and anti-biofilm activity of the lipid extract from Mantidis ootheca on Pseudomonas aeruginosa

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