Antimicrobial, Antifungal & Cytotoxic Activities Screening of Stem Bark Fractions from Terminalia chebula

Abstract

The fractionated crude extracts and two isolated pure compounds TC-1(Phytol) and TC-2(Stigmasterol) from stem bark of Terminalia chebula were screened for their antibacterial and antifungal activities and cytotoxicity against brine shrimp nauplii. Petroleum ether, ethyl acetate (EtOAc) and methanol (MeOH) extracts and the compounds isolated from EtOAC fractions were studied for their antimicrobial activities. Cytotoxic activities were conducted only with crude extract. Both EtOAc and petroleum ether extract showed promising and moderate antimicrobial activities against most of the gram positive and gram negative bacteria whereas the MeOH extract did not show any antimicrobial activities. The isolated pure compounds TC-1 & TC-2 exhibited activities against most of the bacterial strains. The cytotoxicity towards brine shrimp nauplii of the crude extract was studied. The LC 50 values of plant crude extract is found to be 5.623413µg/mL.

A comprehensive review on the diverse pharmacological perspectives of Terminalia chebula Retz

“…Apigenin, a natural flavone from the stem of T. chebula found to be anti-fungal against T. mentagrophytes induced dermatophytosis in mice [ 171 ]. Phytol and stigmasterol isolated from the stem bark of T. chebula showed inhibitory effect on Candida arrizae , Aspergillus niger , C. albicans and Saccharomyces cerevisiae [ 172 ].…”