Antimicrobial, antifungal effect and safety verification using BCOP assay of extracts fromCoptis chinensis

Abstract

Coptis chinensis is used in oriental medicine for soothing, anti-inflammation, antimicrobial and antipyretic properties, and its main ingredient berberine is known to have strong antibacterial activity. In this study, we investigated the antimicrobial effect of hot water extract of Coptis chinensis (CW) on skin related microorganism and the airborne microbe, the antifungal effects of fungi, which are frequently detected in residential environments. CW showed antibacterial effect against Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis, against the airborne microbe, which was collected in four different places. At the concentration of 100 mg/mL, the antimicrobial activity continued for 42 days, showed heat stability without change in the antimicrobial activity even after heat treatment. The MIC and MBC of CW against S. aureus was 0.03, 0.05 mg/mL, against S. epidermidis was 0.50, 0.75 mg/mL and against P. acne was 0.10, 0.15 mg/mL. As a result of measuring the MIC of four kinds of fungi with high detection frequency in the surrounding environment, Gliocladium virens was 65 mg/mL by determined as MIC which can inhibit one hundred percent of mycelial growth. The concentration 90 mg/mL was determined as MIC against Aureobasidium pullulans and 100 mg/mL against Penicilium pinophilum and Chaetomium globosum. CW was considered a safe extract that showed no irritation even in the ocular mucous membrane irritation evaluation test, a patch test. Therefore, these results suggest that Coptis chinensis has antimicrobial, antifungal and safety on human body and can be applied to the development of materials for cosmetic and residential environment industries.

Antioxidant and Anti-inflammatory Responses of Pycnogenol, Hibiscus Flower Extract, and Niacin in Skin Epithelial Cells

“…As NF-κB transcription factors influence the induction of IL-1, IL-6, and IL-8, the inhibitory effects of NF-κB influence anti-inflammatory function [25]. In particular, a substance having an anti-inflammatory action [3] has a soothing effect on the skin. This has also been shown in studies of compounds with anti-inflammatory effects in acne and atopic dermatitis [26].…”

“…As this inflammation also affects the skin, the development of ingredients that suppress inflammatory cytokine production is also crucial for having a beautiful and healthy skin. Anti-inflammatory action is an important factor for resolving skin problems, such as acne and atopic dermatitis, and it also plays a major role in soothing sensitive skin [3].…”