Antimicrobial, antioxidant and anticancer activities of gold nanoparticles green synthesized using Mangifera indica seed aqueous extract

Abstract

In this study, gold nanoparticles (AuNPs) were synthesised using seed extract of mango (Mangifera indica) which is considered as waste and generally thrown away into the environment. The bioactive molecules in the seed act as reducing agent to synthesise AuNPs without using any external agent. The characterisation of green synthesised AuNPs was done using various spectroscopic techniques. Visual colour change from colourless to ruby red colour confirmed the formation of AuNPs which was further confirmed by maximum absorption peak at 550 nm by UV-spectra. Crystalline nature was confirmed by XRD technique while round, triangle and irregular shape and 19.45 nm size was confirmed by TEM and SAED analysis. FTIR analysis confirmed the presence of alcohol or phenol, carboxylic acid, ketones, amines, aromatic amines, aliphatic amines, alkyl halides and alkynes in M. indica seed which were responsible for the reduction of gold to AuNPs. The green synthesised AuNPs were evaluated for their antimicrobial, antioxidant and cytotoxic potential. They showed moderate antibacterial, cytotoxic and dose-dependent antioxidant activity. Seeds of M. indica instead of discarding can be successfully utilised for AuNPs synthesis which can be used as a natural source of antimicrobial, antioxidant and anticancer agent. HIGHLIGHTS Green synthesis of gold nanoparticles (AuNPs) from fruit (Mangifera indica) waste (seed). Characterisation using various spectroscopic techniques: UV-Vis spectroscopy, Zeta potential, FTIR, XRD and TEM analysis. Synthesized AuNPs were round, triangle and irregular in shape and 19.45 nm in size. Antimicrobial activity of AuNPs against 14 microorganisms. Antioxidant activity of AuNPs in terms of DPPH, SO and ABTS. Cytotoxic activity against HeLa, MCF-7 and fibroblast normal cell lines.

Facile green synthesis of silver nanoparticles using Mangifera indica seed aqueous extract and its antimicrobial, antioxidant and cytotoxic potential (3-in-1 system)

“…The zeta potential of AgNPs was 0.67 mV indicating moderate stability. Zeta potential of AgNPs synthesised using Lantana camara leaves was found to be À15.2 mV [25] while AuNPs synthesised from M. indica seed was found to be À1.98 mV [17].…”

“…The peak at 3432 cm À1 and 2926 cm À1 was assigned to O-H stretching of polyphenolic compounds or flavonoids and C-H stretching of proteins respectively [29,30]. M. indica seed powder showed the presence of phenols, flavonoids, tannins and coumarins while its polar solvent methanol extract and aqueous extract revealed the presence of phenols, flavonoids, tannins, cardiac glycosides, coumarins and anthocyanins [17]. The presence of these important secondary metabolites may play a significant role in the formation, reduction, capping and stabilisation of AgNPs.…”

“…Introduction bronchodilatory and laxative [15]. M. indica mediated synthesised AuNPs is reported by [16,17]; however, the efficacy of M. indica seeds to synthesise AgNPs is yet to be studied and perhaps this is the first report of using seed of M. indica for AgNP synthesis. Hence, the aim of the present work was utilisation of M. indica fruit waste (seed) for the synthesis of AgNPs, characterisation by various spectroscopic techniques and evaluation of their antimicrobial, antioxidant and cytotoxic potential (3-in-1 system).…”

Green Synthesis, Characterization and Cholinesterase Inhibitory Potential of Gold Nanoparticles

“…Rajasekharreddy et al [14] In the present study, the Au NPs were prepared by green synthesis, where the plant extracts of D. uncinatum and E. guineense were used as a reducing agent. Green synthesized Au NPs are reported for antibacterial [15,16], antioxidant [17], anticancer [18] etc. but to the best of our knowledge it is not used in Alzeimer disease.…”

Biogenic Metallic Nanoparticles from Seed Extracts: Characteristics, Properties, and Applications

“…Antibacterial activity against Escherichia coli, Staphylococcus aureus, Bacillus cereus (ATCC11778), Bacillus subtilis (ATCC6633), Staphylococcus aureus (ATCC29737), Escherichia coli (NCIM2931), Pseudomonas aeruginosa (ATCC9027), Klebsiella pneumonia (NCIM2719), and Salmonella typhimurium (ATCC23564); antifungal activity against Cryptococcus neoformans (ATCC34664), Candida albicans (ATCC2091), and Candida glabrata (NCIM3438); antioxidant activity; anticancer activity against human gastric cancer cell line (AGS), human cervical cancer cell line (HeLa cells), and breast cancer cell line (MCF-7); antiangiogenic properties [73,93] Apiaceae Carboxyl, alkoxy, alcohols, hydroxyl, and carboxylate…”